A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

The present invention relates to a method for determining the biofilm-forming capacity of microorganisms. The present invention also relates to a method for classifying microorganisms according to the biofilm-forming capacity thereof. In particular, the present invention is useful in the fields of analysis, biological and enzymological research, pharmaceuticals, diagnostics and/or medicine. The present invention is also useful in the clinical, environmental and food-processing fields.

The present invention provides specific peptide biomarkers and sets of peptide biomarkers for use in methods of detecting or identifying bacterial biomarkers in a sample, wherein said bacterial biomarkers can be used to detect Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli, and/or Moraxella catarrhalis in a sample. Kits and diagnostic methods are also provided.

Compounds and complexes that can be useful as enterobactin probes not necessary are disclosed herein. Methods of detecting bacteria and/or methods of determining susceptibility of bacteria to an antibiotic using such compounds and complexes are also disclosed herein.

Methods of detecting and monitoring the progression of liver disease, including time to death, and methods of treating liver disease, in a mammal are provided.

A method for manufacturing a structure for microbe detection comprises the steps of: reacting nitrilotriacetic acid (NTA) and an acid anhydride to prepare a first compound; chelation of metal ions to the first compound to prepare a second compound; binding the second compound and a microbe detector to prepare a third compound; and mixing an exfoliated transition metal-dichalcogenide (TMD) compound and the third compound to prepare a structure for microbe detection, in which the metal ions of the third compound are bound with the transition metal-dichalcogenide compound.

The present invention relates to a membrane based assay method, device and kit for rapid detection and/or quantification of endotoxins in aqueous solutions and test samples. The kit as per the present invention comprises lipopolysaccharide (LPS) affinity ligand conjugated with gold nanoparticles (GNPs); a membrane device comprising an endotoxin affinity membrane positioned parallelly to one or more layer(s) of a hydrophilic material, which are optionally secured in an enclosure; and optionally comprising an indicator chart for quantification of endotoxins in the sample. The method comprises placing the sample suspected of endotoxin contamination on a surface of a membrane comprised in a membrane device; placing once or more a suspension of LPS-affinity ligand conjugated with GNPs over the same area as the sample placed and detecting the presence of endotoxin if the colour signal appears and based on its intensity quantifying the endotoxin levels.

A device for determining presence or absence of infection due to an infectious agent is described. The device comprises a sample receiving zone configured to receive a liquid sample from a subject suspected of having an infection due to an infectious agent, the sample receiving zone positioned to distribute the sample along a first fluid flow path to a first label zone and along a second fluid flow path to a second label zone. Test lines in each fluid flow path capture a mobile detectable species as an indicator of presence or absence of the infectious agent. The device also comprises a reference line positioned in the one of the fluid flow paths. The bidirectional fluid flow paths emanate from a common sample zone and provide an efficient approach to detection and differentiate two species as indicators of the same infectious agent or as indicators of two different infectious agents.

Disclosed herein methods and kits for early detection of huanglongbing (HLB) in a subject at risk for contracting HLB.

Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.