The present invention provides isolated proteins isolatable from a Pasteurella spp. such as P. multocida. Also provided by the present invention are compositions that include one or more of the proteins, and methods for making and methods for using the proteins.

In this application is described a mutant Acinobacter baumannii strain having a deletion or mutation in its genome. The deletion or mutation interrupts the proper translation of the Hcp protein.

Provided herein are methods and compositions for disrupting biofilms in vitro and in vivo. Also disclosed are antibodies comprising a specified heavy chain (HC) immunoglobulin variable domain sequence and/or a specified light chain (LC) immunoglobulin variable domain sequence.

The invention is directed towards an Aerodynamic Automated Biological Assay Device (AABAD) comprising an aerodynamic substrate having a microfluidic cassette and an electronic module, and a system and a method of deploying the same to detect biological agents and hazards suspended in an atmosphere. The AABAD may be in the form/shape of a maple seed/fruit to induce autorotation. A plurality of AABADs are dispersed into the atmosphere from an aircraft or drone. The AABADs rotate via centrifugal forces without motor or active propulsion system while descending to the ground, wherein during the descent, the AABADs microfluidic cassettes collect and process the air samples via a centrifugal force formed from the autorotation generated by the airborne carrier, and to analyze and transmits the results to a remote location.

A diagnostic kit for a confirmatory assay using the indirect ELISA method that measures the levels of anti-Native Hapten antibodies produced during a real infection, thereby preventing large financial losses to livestock farm, by discerning false positives that present anti-LPS antibodies due to doss-reactions with enterobacteria and post-vaccinal antibodies for the diagnosis of bovine brucellosis in blood serum and individual milk (per animal) and bulk milk (tank), characterised by using the crude Native Hapten antigen, extracted from B. melitensis 16M strain, with no purification treatment and an effective adherence capacity, which is used to antigenize plates at a known concentration (1 g per well), where using as reference positive and negative controls subjected to the lyophilisation (freeze drying) method to ensure their preservation, thereby avoiding the contamination and degradation of the antibodies present and ensuring the stability of the optical densities in said controls for a correct results interpretation of an indirect ELISA, are taken as reference. The lyophilisation method for controls that may be used in other diagnostic methods is also presented.

The present invention discloses an enzyme-linked immunosorbent assay (ELISA) kit for Clostridium novyi type B that adopts a suspension of Clostridium novyi type B as a coating antigen. The suspension of Clostridium novyi type B is prepared by the following method: Clostridium novyi type B is inoculated into a medium for enrichment cultivation; the resulting Clostridium novyi type B culture is centrifuged to collect bacteria; and the bacteria are washed and resuspended with carbonate buffer to obtain a suspension of Clostridium novyi type B. The kit of the present invention, which adopts intact Clostridium novyi bacteria as a coating antigen, can detect whether there are anti-Clostridium novyi antibodies in a sheep serum, with high sensitivity and specificity, and thus determine whether the sheep develops black disease. The present invention has detection results of strong specificity and excellent repeatability.

The present invention relates to compositions, methods, and kits for predicting a subject's response to a CAR T cell therapy, by analyzing the intestinal microbiome of the subject. The present disclosure also provides a method of detecting patients at risk for a poor response to CAR T cell therapy by measuring the level of the presently disclosed bacteria or bacterial genes in the microflora or microbiome of a patient receiving or considered for CAR T cell therapy. The present disclosure further provides therapeutic compositions and methods for treating a subject having a cancer, by improving the subject's response to a CAR T cell therapy.

The present invention provides isolated polypeptides isolatable from a Fusobacterium spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.

A method of using microfluidic chips to significantly accelerate the time to identify and quantify microbes in a biological sample and test them for antibiotic resistance, particularly for urinary tract infections. A first microfluidic chip uses antibody or similar probes to identify and quantify any microbes present. The same or a similar chip uses antibody or similar probes to identify microbes with DNA or RNA known to indicate antibiotic resistance. Another microfluidic chip tests for antibiotic susceptibility of any microbes by growing them in very small wells in the presence of antibiotics, reducing the time required for such testing by as much as 95%. Another microfluidic chip runs traditional urinalysis or similar tests.

Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. A Molecular Net may comprise a pseudorandom nature. Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. Porosity, binding affinity, size exclusion abilities, filtration abilities, concentration abilities and signal amplification abilities of a Molecular Net may be varied and depend on the nature of components used in its fabrication. Uses of a Molecular Net may include analyte capture, analyte enrichment, analyte purification, analyte detection, analyte measurement and analyte delivery. Molecular Nets may be used in liquid phase or on solid phases such as nanomaterials, modified metal surfaces, nanospheres, microspheres, microtiter plates, slides, pipettes, cassettes, cartridges, discs, probes, lateral flow devices, microfluidics devices, microfluidics devices, optical fibers and others.