Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

Nanoparticle-based nanosensors comprising supramolecular recognition sequences, protease consensus sequences, post-translationally modifiable sequences, or sterically hindered benzylether bonds for specific interaction with a biological marker, and methods for rapid diagnosis of lung conditions using specified panels of target biomarkers.

A method for forming dendritic mesoporous nanoparticles comprising preparing a mixture containing one or more polymer precursors, a silica precursor, and a compound that reacts with silica and reacts with the polymer or oligomer formed from the one or more polymer precursors, and stirring the mixture whereby nanoparticles are formed, and subsequently treating the nanoparticles to form dendritic mesoporous silica nanoparticles or dendritic mesoporous carbon nanoparticles. The silica precursor may comprise tetraethyl orthosilicate (TEOS), the one or more polymer precursors may comprise 3-aminophenol and formaldehyde and the compound may be ethylene diamine (EDA). There is a window of amount of EDA present that will result in asymmetric particles being formed. If a greater amount of EDA is present, symmetrical particles will be formed.

A fluorescence immunochromatographic detection card and a preparation method therefor and usage thereof is disclosed. The fluorescence immunochromatographic detection card comprises a treatment liquid A, a treatment liquid B, and a detection card. The treatment liquid A contains an antibody 15C4 that is coupled with a fluorescent microsphere. The treatment liquid B contains an antibody 13G12 that is coupled with biotin. The detection card comprises a detection line area and a quality control line area, and a streptavidin detection T line is fixed in the detection area, and an antibody quality control C line is immobilized in the quality control line area. The preparation method comprises: (1) formulating the treatment liquid A; (2) formulating the treatment liquid B; and (3) drawing the line on the detection card. The fluorescence immunochromatographic detection card has characteristics such as high sensitivity, high specificity, and high stability, and can be applied to the rapid detection of disease markers.

Disclosed are functionalizable ligands, nanoparticles, preferably nanocrystals, complexed with ligands and their use for bio-imaging. A nano material includes a nanoparticle and at least one copolymer ligand. A ligand which is a copolymer of general formula (I): HP[(A)x-co-(B)y]n-L-R.

Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can include an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.

Disclosed is a diagnostic kit for quickly diagnosing a target material with high sensitivity using nanoparticles that absorb infrared light and emit infrared light, in which the nanoparticles are maintained in particle size and have enhanced emission intensity.

A fiber fluidic system may be used to produce particles (e.g., NPs and/or microparticles). The fiber fluidic system may include a cylinder with a plurality of elongated fibers oriented along a length of the cylinder. The cylinder may have a first opening at or near a first end of the cylinder and a second opening downstream of the first opening. A constrained phase fluid may be provided through the first opening and a free phase fluid may be provided through the second opening to produce particles (e.g., NPs and/or microparticles) through a second end of the cylinder. The fiber fluidic system may be used to continuously produce the particles at high throughput.

Disclosed is a method of pulsed laser ablation production of gold-platinum Au.sub.xPt.sub.1-x alloy nanoparticles in a colloidal solution. The colloidal solution of Au.sub.xPt.sub.1-x alloy nanoparticles is suitable for many biological applications including lateral flow immunoassays and other bio-detections based on optical scattering. The nanoparticles form by fragmentation of the bulk material without evaporation, minimizing oxidation of the nanoparticles. The nanoparticles conjugate with bio-molecules such as protein, antibodies, peptides, RNA oligomers, DNA oligomers, other oligomers, or polymers effectively by passive adsorption. Advantageously the Au.sub.xPt.sub.1-x alloy nanoparticles have a wide optical extinction spectrum in the visible region, appearing nearly black in both colloidal and dried form. The nanoparticles can be used for labeling bio-molecules and provide a high visual contrast in visual-based bioassays. A combination of the near black color of the Au.sub.xPt.sub.1-x alloy nanoparticles with the red color of pure Au nanoparticles makes multiplexing bio-detection assays possible.

Superparamagnetic nanoparticle-based analytical method comprising providing a sample having analytes in a sample matrix, providing a point of care chip having analytical regions, each of which is a stationary phase having at least one or more sections, labeling each of the analytes with a superparamagnetic nanoparticle and immobilizing the labeled analytes in the stationary phase, providing an analytical device having a means for exciting the superparamagnetic nanoparticles in vitro and a means for sensing, receiving, and transmitting response of the excited superparamagnetic nanoparticles, placing the chip in the analytical device and exciting the superparamagnetic nanoparticles in vitro, sensing, receiving, and transmitting the response of the superparamagnetic nanoparticles, and analyzing the response and determining characteristic of the analytes, wherein the response of the superparamagnetic nanoparticles comprises harmonics. The present invention also provides the hybrid point of care chip and analyzer to be used in the analytical method.