Embodiments of the present disclosure provide bisbiotin ligands and related conjugates and methods. The bisbiotin ligands, combined with streptavidin, can be used in the separation, labelling, targeting, and immobilization of biomolecules.

Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

The present disclosure provides an achromatic colorimetric nanosensor, by using a mixture of nanoparticles with complementary colors. The color changes from an achromatic color to a chromatic color enables more clear color-transition and, thus, allows immediate analysis of the presence of a target analyte. Further, the present disclosure provides achromatic colorimetric nanosensor for detection of multiple analytes using plural nanoparticles via color changes from an achromatic to multiple chromatic colors.

The present application provides compositions for multiplexed imaging using labeled nucleic acid imaging agents.

The present invention in various aspects and embodiments involves pharmaceutical compositions of peptides derived from the 5 fibril of type IV collagen, and uses thereof for medical treatment. The peptides target 51 and V3 integrins, and inhibit signaling through multiple receptors, and find use for inhibiting vascular permeability, angiogenesis, lymphangiogenesis.

This invention is a functionalized diamond crystal with high dispersibility in high ionic strength solution and/or with specific targeting ability, which comprise a diamond crystal and a fatty acid layer. The fatty acid layer works a surfactant and provides a specific targeting feature for the diamond crystal. The feature of the surfactant makes the diamond crystal being easily dispersed in biological surrounding (e.g., phosphate saline buffer) and the feature of specific targeting ability provides the diamond crystal with specific recognizing specific targets. This invention allows researchers to use diamond crystal as a marker for specific labelling.

The present invention provides a fluorescent silica-based nanoparticle that allows for precise detection, characterization, monitoring and treatment of a disease such as cancer. The nanoparticle has a range of diameters including between about 0.1 nm and about 100 nm, between about 0.5 nm and about 50 nm, between about 1 nm and about 25 nm, between about 1 nm and about 15 nm, or between about 1 nm and about 8 nm. The nanoparticle has a fluorescent compound positioned within the nanoparticle, and has greater brightness and fluorescent quantum yield than the free fluorescent compound. The nanoparticle also exhibits high biostability and biocompatibility. To facilitate efficient urinary excretion of the nanoparticle, it may be coated with an organic polymer, such as poly(ethylene glycol) (PEG). The small size of the nanoparticle, the silica base and the organic polymer coating minimizes the toxicity of the nanoparticle when administered in vivo. In order to target a specific cell type, the nanoparticle may further be conjugated to a ligand, which is capable of binding to a cellular component associated with the specific cell type, such as a tumor marker. In one embodiment, a therapeutic agent may be attached to the nanoparticle. To permit the nanoparticle to be detectable by not only optical fluorescence imaging, but also other imaging techniques, such as positron emission tomography (PET), single photon emission computed tomography (SPECT), computerized tomography (CT), bioluminescence imaging, and magnetic resonance imaging (MRI), radionuclides/radiometals or paramagnetic ions may be conjugated to the nanoparticle.

Improved temperature measurement and correction is provided for magnetoresistive sensor arrays. A linear coefficient of resistance vs. temperature is determined from one or more reference sensors in the array by measuring resistance of the reference sensors at known temperatures, which enables resistance measurements to be used to determine unknown temperatures in all sensors of the array. Double modulation leads to MR sensor outputs having center tones which can be used to correct temperature dependence in side tone MR signals. This correction can be according to a linear fit or a polynomial fit. Applications include biological assays requiring accurate temperature data, such as accurate determination of DNA melting curves.

The present invention relates to a method of capturing, enriching, purifying, detecting or measuring a cell in a sample at a sub-nanogram level comprising providing a nanocomposition, contacting the sample with the nanocomposition to form a mixture solution and allowing the binding of the cell with the nanocomposition, applying a magnetic field to the mixture, and evaluating the presence of or absence of the cell. The nanocomposition is capable of capturing or enriching an analyte at a sub-nanogram level, and comprise a nanostructure operably linked to an analyte-capturing member.

Compositions, methods, devices, and systems are described for performing single-step, homogenous, localized surface plasmon resonance (LSPR)-based plasmonic assays having exceptional assay sensitivity and extremely low limits of detection (LODs). Ag/Au core/shell nanoparticles are described, which may be used with LSPR sensors to develop single-step, homogeneous, LSPR-based assays.