The present invention relates to a nanocomposition capable of capturing or enriching an analyte at a sub-nanogram level and methods thereof. The nanocomposition can comprise a nanostructure operably linked to an analyte-capturing member.

Provided are a fluorescent particle and a method for manufacturing the same. The fluorescent particle may include a gold nanoparticle; a silica shell covering the gold nanoparticle; and lanthanide group complex particles dispersed in the silica shell. Each of the lanthanide group complex particles may include a lanthanide group ion; a ligand bonded to the lanthanide group ion and including phosphorus; and a ligand bonded to the lanthanide group ion and having a beta diketone functional group. The fluorescent particle is observable with the naked eye and may emit light when ultraviolet light is irradiated. The fluorescent particle may be used for detecting and analyzing biomaterial samples.

This invention provides a groundbreaking approach to PLNPs and their preparation. In particular, the synthetic methodology disclosed herein fundamentally differs from the traditional solid-state annealing reactions that require extreme and harsh reaction conditions. In one unique aspect of the invention, a simple, one-step mesoporous template method utilizing mesoporous silica nanoparticles (MSNs) is disclosed that affords in vivo rechargeable NIR-emitting mesoporous PLNPs with uniform size and morphology. In another unique aspect of the invention, the novel synthetic approach is based on aqueous-phase chemical reactions conducted in mild conditions, resulting in uniform and homogeneous PLNPs with desired size control (e.g., sub-10 nm).

The present disclosure provides compositions and methods useful for molecular and cellular separation, detection and quantification. The compositions provided herein comprise a nanostructure having magnetic property operably linked to an analyte-binding member.

A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.

Among other things, the present invention provides assay methods for detecting or quantifying one or more analytes in a sample, which involve the use of dissociable nanoparticles that comprise one or more signaling agents (e.g., the nanoparticles conceal or partially conceal the signaling agents).

The invention relates to a method for analysing an interaction between a first molecule and a second molecule bonded to a particle, including the following steps: contacting the first molecule with the second molecule bonded to the particle under conditions enabling the interaction thereof; applying a predetermined liquid flow to the particle bonded to the second molecule; observing a movement of the particle bonded to the second molecule caused by the applied flow; analysing the interaction according to the movement observed and the applied flow, the particle having a greater hydrodynamic resistance than the first and/or second molecule, and a mass Pclet number of greater than 1. The invention also relates to a device for analysing an interaction between a first molecule and at least one second molecule, as well as to the use of the method or of the device in screening a candidate molecule for developing a drug.

A method and a system for detection of presence or absence of analytes in fluids, the method comprising the steps of: a) contacting a fluid sample and a plurality of nanoparticles, the nanoparticles being functionalized with a selective ligand, the contact of the fluid sample with the nanoparticles being under conditions such that, the nanoparticles aggregate selectively on surface of their target analyte, forming a nanoparticle-analyte complex, the aggregation promoting the concentration of the nanoparticles on the surface of the target analyte; b) subjecting a fluid mixture of the fluid sample and the plurality of nanoparticles to SERS; c) measuring at least a SERS signal associated with the fluid mixture; d) spectrally analysing the at least one SERS signal of step c); e) recognizing at least one defined SERS spectrum of the nanoparticle-analyte complex, through a SERS signal enhanced by the at least one narrow inter-nanoparticle gap.

Gold nanoparticles functionalized with thiolated, bidentate Schiff base ligands. The Schiff base ligands form a ligand monolayer surrounding and binding to the surface of a gold nanoparticle core through AuS linkages. The functionalized gold nanoparticle composites have a spherical shape, an average diameter of 7-15 nm and a narrow particle size distribution. Methods of assessing these functionalized gold nanoparticle composites as fluorescent probes in Fe(III) chemosensing applications, methods of preparing the functionalized gold nanoparticle composites and methods of detecting Fe(III) ions with the same are also provided.

Provided herein is a method of effectively quantifying a target material by performing both colorimetry and fluorescence analysis on the same sample, based on metal nanoparticles and an aptamer.