The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.

A sampler and a method of parameterization by calibration of digital circuits and non-invasive determination of the concentration of several biomarkers simultaneously and in real time. The method makes use of equipment which, from a set of luminous signaturesspectrumprovided by a spectrophotometer (E5) (E6), applies a digital filter that breaks down the spectrum into sub-spectra that shows the digital signatures of relevant markers and, through a digital decoder, the concentration of a set of several biomarkers is obtained simultaneously and in real time.

A composition, as well as methods using the composition, for detection or quantification of a molecule at a singlet state (e.g., singlet oxygen). The composition includes one or more nanoparticles, and the nanoparticle has an energy donor, an energy acceptor associated with the energy donor, and an energy transfer mechanism between the energy donor and the energy acceptor.

Provided is a nanoprobe based on a self-replicating biological material. The nanoprobe includes a binding agent existing in a first region of the biological material and capable of binding specifically to a target analyte, and nanoparticles existing in a second region of the biological material and providing an optical detection signal. The use of the nanoprobe enables quantitative analysis and multiplexed analysis of a target. In addition, the nanoprobe is easy to fabricate on a large scale.

Disclosed herein are a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms, and the like, and a multi-functional bio-material conjugate manufactured by means of the same. The method for manufacturing a multi-functional bio-material conjugate includes: (a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. The method for manufacturing a multi-functional bio-material conjugate according to the present invention may prevent precipitation of the nanoparticles, easily immobilize the bio-material, and manufacture a bio-material conjugate having multiple functions, by using two kinds of the particles. In addition, the multi-functional bio-material conjugate manufactured by the present method may be used to detect microorganisms at up to a concentration of 10.sup.1 cfu.

A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonance frequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.

Techniques and systems are disclosed for detecting biomolecular interactions based on the motion of nanomotors. In one aspect, a method of detecting biomolecular interactions based on a motion of a nanomachine includes functionalizing a nanomachine with a capture probe adapted to interact with biological targets; and detecting a presence of the biological targets in an environment based on a motion of the nanomachine.

Some embodiments relate to nanoparticle probes for the detection of disease states in a patient or for tissue engineering. In some embodiments, the nanoparticle probe comprises one or more slip bonds that bind to a cell surface structure. In some embodiments, the binding of the nanoparticle probe is selective. In some embodiments, the nanoparticle probe binds to cells having a certain maximum glycocalyx thickness.

A system for measuring presence and/or amount of a target antibody in a sample comprising a nanoparticle adapted to quench fluorescence emission, an Fc binding protein immobilized or absorbed on the nanoparticle, and a fluorescing antibody adapted for binding to the Fc binding protein and having a lower binding affinity to the Fc binding protein than the target antibody. According to further embodiments of the present invention, methods of measuring presence and/or amount of a target antibody in a sample are also provided. The methods comprise mixing the sample with the components of the system and measuring the change in fluorescent intensity as compared to the total fluorescent intensity before the mixing. The lower affinity of the fluorescing antibody to the Fc binding protein as compared to the target antibody allows for the fluorescing antibody to be replaced by the target antibody and stay unbound in the solution, causing a change in the fluorescence quenching, thereby enabling an estimate on the amount of the target antibody in the sample.

The present invention relates to a nanoparticle heterodimer in which Raman-active molecules are located at a binding portion of the nanoparticle heterodimer, and more particularly, to a core-shell nanoparticle heterodimer comprising: a gold or silver core having a surface to which oligonucleotides are bonded; and a gold or silver shell covering the core. In addition, the present invention relates to the core-shell nanoparticle dimer, to a method for preparing same, and to the use thereof.