The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.

The invention relates to a method of determining glycan moiety on a recombinant glycoprotein using condensation nucleation light scattering detection.

The present invention relates to methods for the detection and quantification of apolipoproteins and isoforms thereof in a sample, as well as to predictive methods of the probability of neurodegenerative or cardiovascular disease development based on apolipoprotein levels as determined by the detection methods of the invention.

Disclosed are methods for identifying one or more amino acid molecules and nucleic acid molecules encoding such amino acid molecules of at least two proteomes that are conserved, unique or express at higher or lower levels in at least one of the proteomes. Expression libraries are used that produce the proteome, and in one embodiment, may produce the proteome from at least one cDNA expression library in one to five reactions. Anti-proteome antibodies are prepared that selectively bind to one of the proteomes and binding with at least one second proteome compared.

The present teachings relate to methods, systems, and kits for analyzing a sample containing a glycopeptide of interest that can subject the glycopeptide of interest to a plurality of parallel deglycosylation reactions that differentially cleave the glycan, with the various products resulting from the deglycosylation reactions being differentially labeled (e.g., with isobaric and/or isomeric labeling reagents) to thereby produce labeled glycopeptides or labeled fragments of the glycopeptides. The products of the various deglycosylation reactions can then be mixed together and subject to LC-MS/MS using a single injection of the mixture. In accordance with various aspects, by associating the resulting mass spectral data with a particular deglycosylation reaction (e.g., based on the presence in the MS/MS data of product reporter ions associated with the particular differential labels), the methods and systems described herein can aid in the identification of the glycan structure

Compositions and methods for enriching glycocompounds are disclosed which can comprise a glycocompound bound to a boronic acid compound which can be conjugated to a dendrimer.

Provided is a means by which a signal-to-noise ratio (S/N ratio) can be improved at the time of detection of lipid bilayer membrane particles (lipid vesicles) in which the amount of a surface antigen is small since the size of the particles is small and/or the amount of the particles existing in a sample is also small.

The present application provides, as a means for solving the above-described problem, a detection technique that relates to a method for detecting lipid bilayer membrane particles or fragments thereof having predetermined molecules existing on surfaces in a biological sample collected from a subject, the method including: adding a dye staining a lipid bilayer membrane to the biological sample; adding a substance specifically binding to the predetermined molecules to the biological sample; trapping the substance to separate the lipid bilayer membrane particles or fragments thereof having predetermined molecules existing on surfaces; and detecting the separated lipid bilayer membrane particles or fragments thereof on the basis of emission of the dye.

Provided herein are methods and systems for proteome analysis that are at least partially automated and/or performed robotically. In some aspects, the methods and systems described herein can rapidly and efficiently provide protein identification of each of the proteins from a proteome, or a complement of proteins, obtained from extremely small amounts of biological samples. The identified proteins can be imaged quantitatively over a spatial region. Automation and robotics facilitates the throughput of the methods and systems, which enables protein imaging and/or rapid proteome analysis.

Provided is a method capable of reducing non-specific adsorption onto a solid phase carrier, and capable of selectively and efficiently separating a vesicle having a lipid bilayer membrane. A method of separating a vesicle having a lipid bilayer membrane includes: a complex forming step of forming a complex of a vesicle having a lipid bilayer membrane and a solid phase carrier to which a ligand which recognizes a surface antigen present on a surface of the vesicle is bound, by bringing a biological sample containing the vesicle into contact with the solid phase carrier; and a washing step of washing the complex, in which at least any one of the complex forming step and the washing step is performed under an environment having a salt concentration of from 0.15 M to 2 M.

The present invention relates to methods for screening a polypeptide for desired activity against a target molecule In particular, the present invention relates to methods for screening a polypeptide for desired activity against a target molecule by expressing the polypeptide in a bacterial cell and permeabilizing the cell.