There is provided a method and materials pertaining to assays, for example immunoassays, for biomarkers in body fluids e.g. blood. Diagnostic or screening methods for infections, and methods of differentiating between infectious and non-infectious conditions in mammals, particularly equines, for monitoring response to anti-infective/antibiotic therapy are provided. A test fluid collection system adapted to permit dilution and analysis of the collected test fluid and an assay and device for monitoring exertional rhabdomyolysis in equines is also provided.

Provided herein are cell-free systems comprising two-component systems including a histidine kinase and a response regulator. In embodiments, the systems further include synthetic membranes. Also provided are methods for using the systems to detect analytes and synthesize target molecules.

A creatine kinase isoenzyme latex-enhanced immunoturbidimetric assay kit, comprising a first reagent and a second reagent. The first reagent comprises a buffer solution, an electrolyte, polyethylene glycol, a surfactant, a preservative, a blocking agent, and a protective agent. The second reagent comprises a buffer solution, polystyrene latex particles coated with a creatine kinase isoenzyme antibody, the creatine kinase isoenzyme antibody on the latex particles, a protective agent, a stabilizer, and a preservative.

The present invention provides a method for determining a mortality risk and/or probability of healthy lifespan of a cat; said method comprising determining the level of one or more biomarker(s) in one or more samples obtained from the cat, wherein the one or more biomarker(s) is selected from white blood cell count, haemoglobin, serum urea nitrogen, serum AST, serum chloride, serum total bilirubin, serum globulin, red blood cell count, serum sodium, serum cholesterol, serum potassium, serum alkaline phosphatase, and/or serum GGT.

The present invention relates to a method for determining a mortality risk and/or probability of a healthy lifespan of a dog; said method comprising determining the level of one or more biomarker(s) in one or more samples obtained from the dog, wherein the one or more biomarker(s) is selected from white blood cell count, serum albumin, serum alkaline phosphatase, serum creatine kinase, haemoglobin, haematocrit, mean corpuscular haemoglobin, serum glucose, mean red cell volume, serum globulin, serum calcium, platelet count, and/or red blood cell count.

According to various aspects, the present invention provides a method of delivering a plurality of sample solutions to a sensor surface (102) for analysis. It can additionally, or alternatively, provide systems and methods for enabling improved investigation of analyte behaviours. The sensor (102) may, for example, be used to determine kinetic binding properties of analytes (such as proteins) to surface bound ligands. In one aspect, a method comprises: a) introducing (902) a first sample solution (502) into a first channel (506), the first channel (506) configured to deliver sample solution to the sensor surface (102); b) introducing (904) a second sample solution (504) into the first channel (506); c) flowing (906) the first sample solution and the second sample solution through the first channel (506) and over the sensor surface (102); and d) detecting (912) the presence or absence of binding of analyte from at least one of the sample solutions (502, 504) at the sensor surface (102), wherein no running buffer solution is passed over the sensor surface (102) throughout steps a)-d).