A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).

A variable focal length (VFL) lens system is utilized to determine surface Z-height measurements of imaged surfaces. A controller of the system is configured to control a VFL lens (e.g., a tunable acoustic gradient index of refraction lens) to periodically modulate its optical power and thereby periodically modulate a focus position at a first operating frequency, wherein the periodically modulated VFL lens optical power defines a first periodic modulation phase. A phase timing signal is synchronized with a periodic signal in the controller that has the first operating frequency and that has a second periodic modulation phase that has a phase offset relative to the first periodic modulation phase. A phase offset compensating portion is configured to perform a phase offset compensating process that provides Z-height measurements, wherein at least one of Z-height errors or Z-height variations that are related to a phase offset contribution are at least partially eliminated.

Provided is a medical observation apparatus including: a columnar microscope unit configured to image a minute part of an object to be observed with magnification and thereby output an imaging signal; and a support unit including a first joint unit holding the microscope unit in a rotationally movable manner around a first axis parallel to a height direction of the microscope unit, a first arm unit holding the first joint unit and extending in a direction different from the height direction of the microscope unit, a second joint unit holding the first arm unit in a rotationally movable manner around a second axis orthogonal to the first axis, and a second arm unit holding the second joint unit. In a plane passing through the first and second axes, a cross section of the microscope unit, the first and second joint units, and the first and second arm units is included in a circle that has a center at a focus position of the microscope unit and passes through an end point of the first joint unit that is at the maximum distance from the focus position. Thus, when imaging an object to be observed and displaying the image, the user's visual field for observing the displayed image can be sufficiently ensured.

The present invention relates to an imaging device for observing the development of a living cell or a set of living cells such as embryos, comprising a lighting system, means for relative displacement and an imaging system equipped with a wide-field camera which is adapted to allow the identification, the imaging and the observation of one or more living cells or sets of living cells to be observed. The invention also relates to a method for observing the development of embryos by means of such a device.

An arrangement for use in illuminating a sample in SPIM microscopy includes an illumination objective configured to receive and focus a light strip or a quasi-light strip. The quasi-light strip is made up of a light bundle continuously moved back and forth in a light-strip plane. A deflection apparatus is configured to deflect the light strip or the quasi-light strip, after the light strip or the quasi-light strip has passed through the illumination objective, in such a way that the light strip or the quasi-light strip propagates at an angle different from zero degrees with respect to an optical axis of the illumination objective. The illumination objective and the deflection apparatus are arranged movably relative to one another.

An optical system includes a scanning unit, a first lens-element group including at least a first lens element, and a focusing unit which is designed to focus beams onto a focus, wherein the focusing unit includes a second lens-element group including at least a second lens element and an imaging lens. The imaging lens further includes a pupil plane and a wavefront manipulator. The wavefront manipulator is arranged in the pupil plane of the imaging lens or in a plane that is conjugate to the pupil plane, or the scanning unit of the optical system is arranged in a plane that is conjugate to the pupil plane and the wavefront manipulator is arranged upstream of the scanning unit in the light direction. The focus of the second lens-element group lies in the pupil plane of the imaging lens in all focal positions of the focusing unit.

A microscope including an illumination objective with a first optical axis, embodied to produce a light sheet, and a detection objective with a second optical axis, embodied to detect light coming from the specimen plane. The illumination objective and the detection objective are aligned relative to one another and the specimen plane so that the first and second optical axes intersect in the specimen plane and include a substantially right angle therebetween. The optical axes each include an angle which differs from zero with a reference axis directed orthogonal to the specimen plane. An overview illumination apparatus for wide-field illumination of the specimen plane, includes an illumination optical unit with a third optical axis. The characterizing feature is that the detection objective is provided to detect both light from the light sheet and light from the illumination optical unit. A method is also provided for operating a light sheet microscope.

An image generation apparatus includes a plurality of irradiators, and a control circuit. The control circuit performs an operation including generating an in-focus image of an object in each of a plurality of predetermined focal planes, extracting a contour of at least one or more cross sections of the object represented in the plurality of in-focus images, generating at least one or more circumferences based on the contour of the at least one or more cross sections, generating a sphere image in the form of a three-dimensional image of at least one or more spheres, each sphere having one of the circumferences, generating a synthetic image by processing the sphere image such that a cross section appears, and displaying the resultant synthetic image on a display.

The invention relates to a method for determining height information of a sample, and to a scanning microscope. The method comprises the following steps: generating an illumination spot; illuminating the sample with the illumination spot; capturing an image of a reflection of the illumination spot at the sample; evaluating the lateral distribution of the image; determining the height information from the lateral distribution; wherein the illumination spot has a three dimensional illumination pattern and/or the image in a detection beam path has a three dimensional detection pattern. The scanning microscope is characterized in that an illumination device (07) and/or a detector device comprise(s) a means for generating a three dimensional pattern with a change in the lateral intensity distribution that is asymmetrical along the optical axis, and an evaluation device is configured to determine height information from the detector signal.

Example methods and systems to obtain images associated with target cells distributed in a cytology specimen have been disclosed. One example method includes obtaining a first image associated with a first region of the cytology specimen through a first object lens, determining a first subregion of the first region, obtaining a second set of one or more images associated with the first subregion through a second object lens, identifying a third image from the first image, and in response to a ratio of a second number of the target cells in the second set of one or more images to the first number of the target cells in the third image being in a predetermined range, obtaining images associated with the target cells based on the second set of images.