Methods and systems for automated slide handling for imaging applications are described herein. In certain aspects, an automated slide handler may be operatively coupled to a slide hotel and/or one or more imaging systems described herein. The automated slide handler may be a robotic arm with up to 6 degrees of freedom. Automated slide handling may include sample preparation, such as sectioning and staining. Suitable imaging systems include a fluorescence microscope or an imaging mass cytometer. Methods and systems disclosed herein enable high throughput profiling of tissue sections.

Disclosed are a photonic crystal microscope and a method of measuring cellular forces. The photonic crystal substrate includes a photonic crystal substrate, a stage, a probe light source, and an imaging assembly, the photonic crystal substrate being disposed above the stage, the probe light source and the imaging assembly being sequentially disposed at a side of the stage opposite the photonic crystal substrate, the photonic crystal substrate being configured to culture a to-be-measured cell, the photonic crystal substrate being deformable when the to-be-measured cell grows on the photonic crystal substrate; the probe light source is configured to emit probe light to the photonic crystal substrate; the photonic crystal substrate is configured to reflect the probe light to the imaging assembly; the imaging assembly is configured to receive the light reflected from the photonic crystal substrate to perform imaging.

Methods and systems are provided for improving resolution, acquisition speed, and/or illumination dose for microscopy systems. In some embodiments, a microscopy system having multiple objective setups may include illumination generators to provide selectively-blanked illumination line scans, objective lenses to introduce the selectively-blanked illumination line scans to a sample and to collect fluorescence emissions from the sample, and detectors to receive the fluorescence emissions from the objective lenses. The microscopy system may also include one or more processors in operative communication with the detectors, which may combine the fluorescence emissions to generate a composite image.

The technology disclosed present systems and methods to produce an enhanced resolution image from images of a target using structured illumination microscopy (SIM). The method includes transforming at least three images of the target captured by a sensor in a spatial domain into a Fourier domain to produce at least three frequency domain matrices that each include first blocks of complex coefficients and redundant second blocks of complex coefficients that are conjugates to the first blocks. The method includes reducing computing resources required to produce the enhanced resolution image by using first blocks of complex coefficients to produce at least three phase-separated half-matrices in the Fourier domain. The method includes performing one or more intermediate transformation on the phase-separated half-matrices to produce realigned shifted half-matrices. The method includes calculating complex coefficients of second blocks in the Fourier domain to produce full matrices from half-matrices.

The present disclosure generally relates to a molecular construct for multiphoton fluorescence microscopy imaging. The molecular construct has a first, non-fluorescent configuration (2PAP-C) and a second, fluorescent configuration (2PAP-CL), and comprises a two-photon absorbing probe (2PAP) linked to a photochromic molecule that can be reversibly changed from a first colored isomeric form (C) to a second colorless isomeric form (CL). The first colored form (C) can be isomerized to the second colorless isomeric form (CL) upon absorption of two photons by the two-photon absorbing probe (2PAP). The present disclosure also relates to a method for analyzing a target structure in a multiphoton microscope utilizing the molecular construct. Furthermore, the present disclosure relates to an antibody tagged with the molecular construct, and to the use of the molecular construct for imaging a target structure.

The present invention relates to a method of reading out information from a data carrier and to a data carrier utilizing the concept of structured-illumination microscopy or saturated structured-illumination microscopy.

A fluorescence observation apparatus (1) includes an irradiation unit (10) that applies a plurality of kinds of excitation light (L11, L21) of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample (5) that is labeled with a composite phosphor containing two or more kinds of fluorescent molecules (A, B) at a predetermined composition ratio, a detection unit (20) that detects fluorescence (L12, L22) generated at each of the plurality of positions by application of the irradiation unit (10), and a calculation unit (33) that determines a distribution of pieces of the composite phosphor on the basis of a fluorescence signal (S1, S2) that is obtained from a detection result of the detection unit (20) and that shows a fluorescence intensity corresponding to a position in the biological sample (5) of each piece of the fluorescence (L12, L22).

A method and a device are useful for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid (DNA) using Crithidia luciliae cells by fluorescence microscopy and by digital image processing.

According to the present disclosure, an optical signal emitted from a single molecule is received to measure a central location of the single molecule while changing a phase of a structured illumination having a periodic pattern to measure a phase of a pattern in which a fluorescence intensity is periodically changed in accordance with a distance between the pattern and the single molecule while displacing the periodic pattern by a specific interval to measure the central location of the single molecule, thereby improving an accuracy of the central location of the single molecule with low photons and as a result, the resolution of the image may be enhanced.

A method is provided comprising: positioning a slide that includes a concave region that is formed in a top surface of the slide and that can contain an object, such that a focal point of an objective lens of a fluorescence lifetime imaging microscopy (FLIM) device is within an area of the concave region between a bottom surface of the concave region and the top surface of the slide; and using the FLIM device to capture a sequence of wide field images of a portion of the object within a field of view of the objective lens.