The invention relates to methods of treating baldness, treating alopecia, promoting hair growth, and/or promoting hair follicle development and/or activation or stimulation on an area of the skin of a subject (for example, a human) by subjecting said area of the skin to integumental perturbation. Integumental perturbation can be used in combination with other treatments for promoting hair growth. The invention provides devices for integumental perturbation for promoting hair growth, and provides pharmaceutical compositions for use in combination with integumental perturbation for promoting hair growth.

A formulation for the topical treatment of the skin comprises an extract of Centella asiatica, a source of phytosterols with anti-oxidant and anty-inflammatory activity comprising Urtica dioica and hyaluronic acid or a salt thereof.

A formulation for the topical treatment of the skin comprises an extract of Centella asiatica, a source of phytosterols with anti-oxidant and anty-inflammatory activity comprising Urtica dioica and hyaluronic acid or a salt thereof.

A freeze-dried formulation and a preparation method and an application thereof are provided. Triterpenoid saponin has a mass percentage of 0.004-95% in the freeze-dried formulation, a binding agent has a mass percentage of 0.01-99% in the freeze-dried formulation; the freeze-dried formulation is freeze-dried by a primary prepared solution containing the triterpenoid saponin and the binding agent; when the triterpenoid saponin has a mass percentage of 0.001%-50% in the primary prepared solution, the binding agent has a mass percentage of 0.01%-50% in the primary prepared solution, and a mass of the triterpenoid saponin increases with an increase of a mass of the binding agent; and when the triterpenoid saponin has a mass percentage of 50%-95% in the primary prepared solution, the binding agent has a mass percentage of 0.01%-20% in the primary prepared solution, and the mass of triterpenoid saponin decreases with the increase of the mass of the binding agent.

A composition for preventing hair loss and, more specifically, to a compound having a structure in which finasteride and a peptide are linked through a covalent bond and a pharmaceutical composition or a cosmetic composition for preventing hair loss or promoting hair growth comprising the same. The compound having a structure in which finasteride and a peptide are linked through a covalent bond is excellent in physiological activities such as hair loss improvement, hair growth promotion, cell growth promotion, etc., is excellent in stability in water and skin permeation, and thus can be effectively used as a composition for preventing hair loss and promoting hair growth.

A composition for preventing hair loss and, more specifically, to a compound having a structure in which finasteride and a peptide are linked through a covalent bond and a pharmaceutical composition or a cosmetic composition for preventing hair loss or promoting hair growth comprising the same. The compound having a structure in which finasteride and a peptide are linked through a covalent bond is excellent in physiological activities such as hair loss improvement, hair growth promotion, cell growth promotion, etc., is excellent in stability in water and skin permeation, and thus can be effectively used as a composition for preventing hair loss and promoting hair growth.

A method of culturing natural killer (NK) cells according to an embodiment of the present disclosure can be used in immunotherapy. An additive composition for an NK cell culture medium according to an embodiment of the present disclosure may inhibit the activity of CD4+ T cells, thereby effectively helping NK cell proliferation and activation.

The present invention provides a water-in-oil emulsion composition having a high water occlusive of a coating film and having good skin protective feeling and glossiness. The water-in-oil emulsion composition is provided containing the following components (A), (B), (C), (D) and (E): (A) 1% by mass or more and 15% by mass or less of a lipophilic surfactant having an HLB of 2-6; (B) 0.15% by mass or more and 8% by mass or less of a dextrin fatty acid ester having 8-22 carbon atoms in the fatty acid moiety; (C) 1% by mass or more and 20% by mass or less of one or more oily components selected from a dimer acid ester, a fatty acid cholesterol ester having 16-22 carbon atoms, a fatty acid phytosterol ester having 16-22 carbon atoms, and an N-acyl amino acid ester; (D) a hydrocarbon oil that is liquid at 25° C.; and (E) water.

According to some embodiments, a method of promoting hair growth or hair stimulation in a subject comprises applying vacuum or suction using a handpiece assembly along a targeted portion of the subject's skin surface where hair growth or hair stimulation is desired and providing at least one treatment material to said targeted portion of the subject's skin surface, wherein the application of vacuum or suction helps promote hair growth or stimulate hair.

The present invention provides a topical formulation for use in a method of treating or preventing a sebaceous gland disorder selected from acne, seborrhea, rosacea, perioral dermatitis, corticosteroid-induced acneiform lesions and oily skin and/or for use in a method of inhibiting growth of riopionibacterium acnes, said method comprising topically administrating a formulation comprising (i) cannabidiol and (ii) triterpenes selected from asiaticoside, madecassoside, asiatic acid, madecassic acid and combinations thereof.

The invention further relates to a topical formulation comprising: cannabidiol; triterpenes selected from asiaticoside, madecassoside, asiatic acid, madecassic acid and combinations thereof; flavonolignans selected silibinin, isosilibinin, silicristin, silidianin and combinations thereof, the concentrations of these flavonolignans being calculated as silibinin.