A nano composite material aiming at an acidic sealing zone in osteoclasts and a preparation method thereof are provided. The nano composite material aiming at the acidic sealing zone in the osteoclasts includes a nano material, bone-targeting molecules, and a compound able to react with the acidic sealing zone in the osteoclasts, wherein: after being modified by the bone-targeting molecules, the nano material is loaded with the compound able to react with the acidic sealing zone in the osteoclasts. Through accurate mature osteoclast targeting and chemically regulated biocascade effects, the osteoclasts are inhibited, which provides a new idea and a new tool for drug therapy of abnormal osteoclast activation.

Disclosed is a reaction device, comprising: a reactor body (100) and a supply device (200), wherein the reactor body (100) has a first end (106) and a second end (107) and is used for accommodating a reaction liquid, with a first injection port (101) being provided between the first end (106) and the second end (107), and a discharge port (109) being provided at the second end (107); and the supply device (200) is in communication with the first injection port (101) to inject a continuous phase, wherein the continuous phase directionally flows in the reactor body (100) to form or maintain a parameter gradient in the reactor body (100). By means of injecting the continuous phase into the first injection port (101) on the reactor body (100), the solution presents a certain parameter gradient on two sides of the first injection port (101) in the reactor body (100).

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

The present invention provides a method for producing lipid particles that have a desired particle diameter, the method comprising: (A) a step for preparing, in a first mixing region, a primary dilution by mixing a first solution containing a lipid and an alcohol with a second solution containing water; (B) a step for feeding the primary dilution from the first mixing region to a second mixing region through a liquid feed pipe within a prescribed time; and (C) a step for preparing a secondary dilution by mixing the primary dilution with a third solution containing water in the second mixing region, wherein the steps (A)-(C) are continuously carried out, and the particle diameter of the lipid particles is controlled by adjusting at least one condition selected from the group consisting of the concentration of the alcohol and the concentration of the lipid in the primary dilution, the prescribed time, and the temperature during mixing.

The present invention relates to a method for producing polymersomes comprising a finalisation step using a dual centrifuge (DC) or a dual asymmetric centrifuge (DAC), polymersomes obtainable by said method and their use as a medicament.

A filter holder for liposome extrusion includes a housing having an inlet configured to receive a material to be extruded and an outlet, and a filter support member disposed within the housing between the inlet and the outlet. The filter support member includes an upstream side having a filter support surface configured to support a membrane filter assembly, a downstream side opposite the upstream side, and a plurality of passages extending through the filter support member from the filter support surface to the downstream side. The filter holder also includes an outlet cavity in fluid communication with the outlet, and the filter holder is configured such that the material to be extruded flows through the membrane filter assembly and into the outlet cavity via the plurality of passages before being discharged through the outlet.

The present disclosure provides methods for preparing vesicles. In some embodiments, the methods involve providing a molten mixture of vesicle forming lipids and then adding the molten mixture to an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. In other embodiments, the methods involve providing a lyophilized lipid product and rehydrating the lyophilized lipid product with an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. The lyophilized lipid product is prepared by melting vesicle-forming lipids to produce a molten lipid mixture and then lyophilizing the molten lipid mixture. The present disclosure also provides antigen-containing vesicle formulations prepared using these methods. The present disclosure also provides kits that include a lyophilized lipid product in a first container and an aqueous solution comprising an antigen in a second container.

A multivesicular liposome composition includes a plurality of multivesicular liposomes. Each multivesicular liposome includes a carrier liposome particle including carrier phospholipid bilayer and having an average diameter less than 1 micron; and at least one sub-chamber liposome nanoparticle including a sub-chamber liposome nanoparticle bilayer and having an average diameter less than about 50 nm. The carrier liposome particle encapsulates the at least one sub-chamber liposome nanoparticle. Characteristically, tail groups of the carrier liposome phospholipids are larger than tail groups phospholipids of the at least one sub-chamber liposome nanoparticle.

A method for localizing delivery of an agent to a target site in a subject is provided. The method allows accumulation and/or release of the agent at the target site in the subject through the use of an energy source.

A preparation method of a colloidal system of stabilisation and controlled release of royal jelly ingredients, wherein the system is characterized by the extraction of the components of the royal jelly and the simultaneous encapsulation in combinatorial liposome -cyclodextrin carriers for achieving controlled release, taking advantage of the special features of both carriers, namely: the ability of cyclodextrins to encapsulate polyphenols and their enhanced skin penetration ability as well as the ability of liposomes in encapsulating large amounts of components of various degrees of polarity, for local component transport and for controlled release rate.