The present invention provides an apparatus for detecting photoluminescent light emitted from a sample, said apparatus (200; 300) comprising at least one light source (210; 310, 318), which is configured to emit light of a first and a second wavelength towards a sample comprising photoluminescent particles, wherein said first wavelength is an excitation wavelength for inducing photo-luminescent light from said photoluminescent particles, and wherein said second wavelength is longer than said first wavelength and for gathering background noise information from said sample. The apparatus further comprises a photo-detector (206) for detecting light incident on the photo-detector (206); and an interference filter (204; 304) arranged on the photo-detector (206), wherein the interference filter (204; 304) is configured to selectively collect and transmit light towards the photo-detector (206) based on an angle of incidence of the light towards the interference filter (204; 304), wherein the interference filter (204; 304) is configured to selectively transmit supercritical angle light from the sample towards the photo-detector (206) and suppress undercritical angle light from the sample.

Systems and methods include a fluorescence measurement apparatus. A single-wavelength light source generates an excitation light source. A sample holder holds a sample and includes a surface transparent to the excitation light source. Mounts attached to the single-wavelength light source(s) or the sample holder change an incident angle of the excitation light source on the surface. Optical components positioned in a path of a fluorescence emission emitted from the surface guide the fluorescence emission to a detector that obtains spectra from at least first and second angles-of-incidence. A device records spectra obtained by the detector from the first and second angles-of-incidence, normalizes and analyzes intensities of the spectra, subtracts a first spectrum corresponding to the first angle-of-incidence from a second spectrum corresponding to the second angle-of-incidence to obtain a difference, identifying a sample type of the sample based on an API gravity mapped to the difference.

A multimodality system includes first and second modalities, a catheter, and a processor. The catheter collects fluorescent light from a plurality of locations of a sample which has been irradiated with excitation light of the second modality; a detector detects intensity of the fluorescent light received from the plurality of locations as a function of an angle α formed between the normal to the sample surface and the optical axis of the excitation light. A processor calculates the angle α at each of the plurality of locations based on radiation of the first modality incident on the sample, and corrects the intensity of the detected fluorescent light using a calibration factor g(α). The calibration factor g(α) is a function of the angle α calculated at two or more of the plurality of locations. The angle α is composed of a transversal angle α.sub.t and an axial angle α.sub.a.

This disclosure relates generally to optical trains for imaging systems. More particularly, this disclosure relates to imaging systems configured to limit optical aberrations. Furthermore, this disclosure relates to methods of limiting optical aberrations in imaging systems configured for imaging a sample, though more specifically, for detecting individual detection moieties within a plurality of detection moieties.

A detection device includes a light emitting element, an accommodation frame, a light detector, and a movable light splitter. The light emitting element provides an excitation beam. The accommodation frame accommodates an object under test, and a portion of the excitation beam whose dominant light emitting wavelength falls within a first waveband range forms a fluorescent beam after passing through the object under test. The light detector receives a portion of the fluorescent beam whose dominant light emitting wavelength falls within a second waveband range. The movable light splitter forms a plurality of sub-beams from an incident beam. The sub-beams have respectively different dominant light emitting wavelengths and exits at different emitting angles. The incident beam is at least one of the excitation beam and the fluorescent beam.

A microscopic observation apparatus that irradiates an observation target with excitation light to observe fluorescence generated from the observation target, the microscopic observation apparatus includes a light source that irradiates the observation target with excitation light; a first optical system that light-controls a plurality of light rays including fluorescence generated from the observation target by radiating the excitation light and part of the excitation light; a filter that reduces an intensity of light in a wavelength band of the excitation light among the plurality of light rays light-controlled by the first optical system; and a plurality of photoelectric conversion elements that converts a plurality of light rays that has passed through the filter into electricity.

A photonic system and a method for analysing target molecules that emit radiation upon being exposed to excitation radiation includes a radiation source configured to provide excitation radiation with a predetermined polarization state, and a photonics chip that includes an analysis region for exposing target molecules to radiation from the radiation source, so that target molecules with a dipole component along the direction of polarization of the excitation radiation emit radiation with an angular profile. A waveguide structure captures radiation emitted from the analysis region by target molecules. The waveguide structure is configured to extract the emitted radiation in different positions in accordance with the angular profile of the emitted radiation for sampling the angular profile.

An apparatus for detecting fluorescent light emitted from a sample comprises: a light source, which is configured to emit excitation light of an excitation wavelength towards a sample comprising fluorophores such that fluorescent light is induced; a photo-detector for detecting light incident on the photo-detector; and an interference filter arranged on the photo-detector, wherein the interference filter is configured to selectively collect and transmit light towards the photo-detector based on an angle of incidence of the light towards the interference filter, wherein the interference filter is configured to selectively transmit supercritical angle fluorescence from the sample towards the photo-detector and suppress undercritical angle fluorescence from the sample.

The present application discloses a portable ultraviolet excited fluorescence intensity detector for carrying out quantitative fluorescence detection for a disposable hygiene product's raw materials and a method of using the same, comprising a housing, an ultraviolet emitting light path, a first receiving light path and a second receiving light path

An assay device is disclosed comprising a housing and a test portion, electronic circuitry and an optical assembly each a least partially located in the housing. The test portion comprises one or more test zones adapted to receive an analyte and a fluorescent label associated with the analyte, the fluorescent label being excitable by excitation light and adapted to emit emission light upon excitation by excitation light. The electronic circuitry comprises one or more light sources and one or more light detectors. The optical assembly comprises one or more excitation light guides adapted to guide excitation light from the one or more light sources to the one or more test zones, and/or one or more emission light guides adapted to guide emission light from the one or more test zone to the one or more light detectors.