Methods and systems for synthesizing multicompartment capsules are disclosed, as well as multicompartment polymer capsules formed in accordance with disclosed techniques. At least one plurality of polymer capsules are formed via a capsule-forming process. A feed solution and a reservoir solution are provided, each comprising a biopolymer. The feed solution biopolymer and the reservoir solution biopolymer have opposite charges. Droplets of the feed solution are introduced into the reservoir solution, thereby forming via electrostatic complexation a plurality of polymer capsules. At least a portion of the resulting polymer capsules are then encapsulated in a larger polymer capsule via a similar process, wherein the feed solution utilized for the encapsulation process also comprises the formed smaller capsules.

Disclosed herein is a method for generating capsules with a hydrogel matrix. The method includes the steps: providing in a first chamber a dispersed aqueous phase, the dispersed aqueous phase including water and a hydrogel matrix-forming agent, in which the hydrogel matrix-forming agent is configured to form a hydrogel matrix upon exposure to a gelation inducer; providing in a second chamber a continuous oil phase, the continuous oil phase including oil and at least one first surfactant. The first and second chambers are fluidic connected by one or more channels, preferably by micro-channels. The method further includes: guiding the dispersed aqueous phase from the first chamber through the one or more channels into the second chamber to form an emulsion or dispersion of the dispersed aqueous phase in the continuous oil phase and exposing the hydrogel matrix-forming agent to a gelation inducer to form capsules with a hydrogel matrix.

Various embodiments of the present invention are directed to the field of treating and preventing osteoporosis, with particular embodiments directed to a method of ameliorating, treating, or preventing osteoporosis in a human subject employing tomatidine, xylitol, rapamycin, etc., as well as modifying an individual's microbiome to reduce the likelihood of osteoporosis.

The present disclosure relates to compositions and methods for treating autism spectrum disorder (ASD) by restoring an ASD patient's gut microbiota. These methods can be used with ASD patient with or without ongoing gastrointestinal symptoms. Provided here is a method for ASD treatment in a subject in need thereof comprising or consisting essentially of administering a therapeutic composition comprising a fecal microbe or a fecal microbiota preparation to the subject. Also provided here is a method comprises administering an antibiotic to a human subject; subjecting the human subject to a bowel cleanse; and administering purified fecal microbiota to the human subject. Further provided are evaluation and quantitative characterization of patient symptom improvements upon treatment described here.

Disclosed herein are methods and compositions for the emulsification of solid supports in deformable gel beads. The methods and compositions provided herein may be used in microfluidic systems and devices. In some aspects of the disclosure, deformable gel beads containing solid supports may be paired with single cell entities. The methods and compositions provided herein may be suitable for single cell analysis, including, but not limited to, labeling single cells or components thereof for downstream analysis.

Disclosed are methods and compositions of microbead carriers for delivery of cells and other biologically active substances to diseased or damaged tissue in a subject in need thereof.

The present disclosure describes a method of encapsulating cells in a crosslinked alginate microbead using a microfluidic encapsulation device.

According to the present invention, there are provided a membrane for immunoisolation, including: a porous membrane that contains a polymer, in which the porous membrane includes a layered compact portion where a pore diameter is the smallest within the membrane, and a pore diameter continuously increases in a thickness direction from the compact portion toward at least one surface of the porous membrane; a chamber for transplantation for enclosing a biological constituent therein, including the above-described membrane for immunoisolation on at least a part of a surface forming an inside and an outside of the chamber for transplantation; and a device for transplantation, including the above-described chamber for transplantation enclosing the biological constituent therein. In the membrane for immunoisolation of the present invention which can be manufactured at low costs, a deterioration in substance permeability is unlikely to occur.

The present invention relates to the use of scaffolds to enhance the viability of cells implanted in the integumentary system such that the cell may release an agent. The scaffold is capable of protecting the cell, as well as allowing for adequate nutrient delivery at the implant site through vascularisation in and around the scaffold.

The current invention relates to the field of a novel probiotic-curcuminoids formulation, which exhibits increased curcuminoids bioavailability, and viable probiotic microbial population in the presence of curcuminoids. The probiotic-curcuminoids formulation is made by encapsulation in ragi and galactomannan-rich fenugreek dietary fiber matrix. Compositions and methods for making this formulation are also disclosed herein.