The present invention provides formulation/composition comprising phytonutrients with natural chlorotoxins for targeting cancer, infection, inflammation and pain without any side effects and a method for synthesizing the same. The raw materials are cleaned and dried and to prepare coarse powder of 40 mesh size, extracted with solvent (comprising water:alcohol in a ratio of 40:60) in a ratio of 4:1 with overnight soaking. Prior to cold extraction, the mixture is macerated for 4 hours. The mixture is refluxed for 2 hours at 80 C. The addition of ethanol, maceration and refluxing steps are repeated three times and above solvent is added, if required. The residue is checked for complete extraction after every refluxing step. The extract/residue is filtered and concentrated under vacuum. The extract/residue is vacuum tray dried at 70-80 C. for 12 hours. The extract/residue is scraped and dried lumps of the extract/residue are milled. The extract/residue is sieved and packed.

The present invention provides formulation/composition comprising phytonutrients with natural chlorotoxins for targeting cancer, infection, inflammation and pain without any side effects and a method for synthesizing the same. The raw materials are cleaned and dried and to prepare coarse powder of 40 mesh size, extracted with solvent (comprising water:alcohol in a ratio of 40:60) in a ratio of 4:1 with overnight soaking. Prior to cold extraction, the mixture is macerated for 4 hours. The mixture is refluxed for 2 hours at 80 C. The addition of ethanol, maceration and refluxing steps are repeated three times and above solvent is added, if required. The residue is checked for complete extraction after every refluxing step. The extract/residue is filtered and concentrated under vacuum. The extract/residue is vacuum tray dried at 70-80 C. for 12 hours. The extract/residue is scraped and dried lumps of the extract/residue are milled. The extract/residue is sieved and packed.

The present invention provides formulation/composition comprising phytonutrients with natural chlorotoxins for targeting cancer, infection, inflammation and pain without any side effects and a method for synthesizing the same. The raw materials are cleaned and dried and to prepare coarse powder of 40 mesh size, extracted with solvent (comprising water:alcohol in a ratio of 40:60) in a ratio of 4:1 with overnight soaking. Prior to cold extraction, the mixture is macerated for 4 hours. The mixture is refluxed for 2 hours at 80 C. The addition of ethanol, maceration and refluxing steps are repeated three times and above solvent is added, if required. The residue is checked for complete extraction after every refluxing step. The extract/residue is filtered and concentrated under vacuum. The extract/residue is vacuum tray dried at 70-80 C. for 12 hours. The extract/residue is scraped and dried lumps of the extract/residue are milled. The extract/residue is sieved and packed.

The invention refers to a process for the production of a continuous cell line of simile embryo stem cells, such as Amblyomma sculptum (Acari: Ixodidae), known as line IBU/ASE-16 (access number with CNCM: 1-5000) and its uses. More specifically, the invention refers to a process for the production of the line IBU/ASE-16 and their use for obtaining extracts for the production of vaccines and candidate recombinant proteins for biopharmaceuticals and acaricides, production of diagnostic kits for the detection of antigens and/or antibodies for animal and human use, obtaining clones for use in genotyping and use as a substrate for the isolation and culture of pathogens.

The invention refers to a process for the production of a continuous cell line of simile embryo stem cells, such as Amblyomma sculptum (Acari: Ixodidae), known as line IBU/ASE-16 (access number with CNCM: 1-5000) and its uses. More specifically, the invention refers to a process for the production of the line IBU/ASE-16 and their use for obtaining extracts for the production of vaccines and candidate recombinant proteins for biopharmaceuticals and acaricides, production of diagnostic kits for the detection of antigens and/or antibodies for animal and human use, obtaining clones for use in genotyping and use as a substrate for the isolation and culture of pathogens.

Disclosed is a topical drug for treating breast cancer and preparation method thereof. The topical drug comprises the following ingredients in parts by weight: 5-10 parts of Astragalus, 3-5 parts of Oldenlandia diffusa, 3-5 parts of dandelion, 5-10 parts of Lycoris radiate, 5-8 parts of Carapax trionycis, 3-5 parts of Pinellia pedatisecta, 2-3 parts of Acorus calamus, 3-5 parts of Ferula sinkiangensis, 3-5 parts of doubleteeth pubescent angelica root, 4-6 parts of Cryptotaenia japonica Hassk, 3-5 parts of Whitmania pigra Whitman, 5-8 parts of Wedelia chinensis, 6-8 parts of Echinacea purpurea, 4-6 parts of Folium Eriobotryae, 3-5 parts of Sedum sarmentosum, 5-10 parts of Angelicae sinensis, 2-5 parts of asparagus, 2-3 parts of scorpio, 5-8 parts of Rubia cordifolia L., 2-3 parts of alfalfa extract, 3-5 parts of extract of Jew's ear from mulberry trees, 5-7 parts of myrrh, 3-6 parts of nutgrass galingale rhizome and 3-5 parts of pericarpium citri reticulatae viride.

Disclosed is a topical drug for treating breast cancer and preparation method thereof. The topical drug comprises the following ingredients in parts by weight: 5-10 parts of Astragalus, 3-5 parts of Oldenlandia diffusa, 3-5 parts of dandelion, 5-10 parts of Lycoris radiate, 5-8 parts of Carapax trionycis, 3-5 parts of Pinellia pedatisecta, 2-3 parts of Acorus calamus, 3-5 parts of Ferula sinkiangensis, 3-5 parts of doubleteeth pubescent angelica root, 4-6 parts of Cryptotaenia japonica Hassk, 3-5 parts of Whitmania pigra Whitman, 5-8 parts of Wedelia chinensis, 6-8 parts of Echinacea purpurea, 4-6 parts of Folium Eriobotryae, 3-5 parts of Sedum sarmentosum, 5-10 parts of Angelicae sinensis, 2-5 parts of asparagus, 2-3 parts of scorpio, 5-8 parts of Rubia cordifolia L., 2-3 parts of alfalfa extract, 3-5 parts of extract of Jew's ear from mulberry trees, 5-7 parts of myrrh, 3-6 parts of nutgrass galingale rhizome and 3-5 parts of pericarpium citri reticulatae viride.

Provided herein are compositions and methods for reducing bacterial contamination during cell culture. Such compositions and methods utilize engineered peptides or recombinant cells capable of secreting such peptides into culture medium. Also provided are methods of using the engineered peptides for inhibiting bacterial growth during culturing of cells.

Provided herein are compositions and methods for reducing bacterial contamination during cell culture. Such compositions and methods utilize engineered peptides or recombinant cells capable of secreting such peptides into culture medium. Also provided are methods of using the engineered peptides for inhibiting bacterial growth during culturing of cells.

A composition for treating vitiligo and a method of making the same, said composition comprising: a blue scorpion venom; and an alpha lipoic acid. Preferably, the blue scorpion venom is diluted, polarized, and nanosized. Preferably the alpha lipoic acid is polarized and nanosized. The composition may also comprise various vitamins and minerals.