Methods for identifying one or more Pol inhibitors and methods of use thereof are disclosed.

Systems and methods for nucleic acid sequencing are provided. Polynucleotide strands to be sequenced are immobilized to a surface, clonally amplified and primed for polynucleotide synthesis. Nucleotides modified with cleavable electroactive labels at the 3-OH of the sugar ring are provided and are incorporated into the growing strand during synthesis. The labels of nucleotides that are successfully incorporated into the growing polynucleotide strand are cleaved via application of a stimulus after incorporation and are detected by an electronic nanosensor. The systems and methods described herein combine the high accuracy of SbS methods combined with the scalability and speed of semiconductor-based electrical detection mechanisms.

A method of selective DNA amplification of a DNA mixture comprising a first population of DNA and a second population of DNA, wherein: a) a catalytically dead Staphylococcus aureus Cas9 complex (dSaCas9) comprising a dSaCas9 protein or derivative thereof complexed with one or more guide RNAs having selective binding affinity for DNA sequences preferentially present in the first population of DNA compared to the second population of DNA is contacted with the DNA mixture under a first reaction condition, said first reaction condition being suitable for binding of the dSaCas9 complex to DNA sequences for which it has a binding affinity, and then b) a strand-displacing DNA polymerase is contacted with the DNA mixture under a second reaction condition, said second reaction condition being suitable for amplification being suitable for amplification activity of the strand-displacing DNA polymerase; and related kits and uses.

In various embodiments alpha helical peptide-based bridges for molecular biosensing on-chip are disclosed. The Peptide-based bridges serve as common bridges for great diversity of biosensing applications and targets including nucleic acids, proteins, antigens, antibodies, small molecules. The primary sensor element is preferably a molecular wire such as an alpha-helical peptide integrated into a current monitoring circuit. The engineered peptide may contain a central conjugation site for attachment of various probe molecules including nucleic acids, proteins, antigens, antibodies. The probe-containing bridge empowers the sensor to detect interactions with specific target molecules.

The present disclosure relates, in some embodiments to compositions comprising a variant Cas12a and one-pot methods of using a variant Cas12a with amplification to detect a sequence of interest.

The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.