Disclosed is an in vitro method for detecting, in a biological sample, the presence of a blood coagulation enzyme inhibitor selected, independently, from factor Xa and factor IIa, the method including the step of making one or more competitive kinetics measurement(s) by carrying out a competitive enzymatic assay and implementing one or more types of classification or regression decision models obtained by training automated supervised learning models. The method may include the identification in the biological sample of an inhibitor in question, or even its characterization, or even its quantification. Also disclosed is a data processing system or device including implementation for at least part of the method, a computer program or storage medium which can be read by a computer or appropriate data, and a suitable kit.

The present invention provides methods for detecting a complex with low affinity, under conditions in which the binding equilibrium of the complex is substantially maintained, and methods for measuring the concentration and/or amount of the complex. The invention also provides methods for evaluating the kinetics of a complex and methods for deciding on a therapeutic method that uses a pharmaceutical agent, based on the concentration and/or amount of the complex determined by the above-mentioned measurement method.

Disclosed is an in vitro method for detecting, in a biological sample, the presence of a blood coagulation enzyme inhibitor selected, independently, from factor Xa and factor IIa, the method including the step of making one or more competitive kinetics measurement(s) by carrying out a competitive enzymatic assay and implementing one or more types of classification or regression decision models obtained by training automated supervised learning models. The method may include the identification in the biological sample of an inhibitor in question, or even its characterization, or even its quantification. Also disclosed is a data processing system or device including implementation for at least part of the method, a computer program or storage medium which can be read by a computer or appropriate data, and a suitable kit.

Disclosed herein are devices for measuring, at one or more time points, one or more properties or changes in properties of a fluid sample. The devices may comprise a chamber defining an internal volume of the device suitable for receiving and retaining the fluid sample; a plurality of layers, the plurality comprising at least a bottom layer below the chamber and at least a substrate layer above the chamber, wherein: the substrate layer is linked to at least one suspended beam at each end of its length; the suspended beam is located above the chamber, the suspended beam having a face capable of physical contact with the fluid sample; and the suspended beam is configured to oscillate upon application of an actuating signal to at least one electrically conductive path, which runs across the suspended beam. Related methods and uses are also disclosed.