A miniature electrode apparatus is disclosed for trapping charged particles, the apparatus including, along a longitudinal direction: a first end cap electrode; a central electrode having an aperture; and a second end cap electrode. The aperture is elongated in the lateral plane and extends through the central electrode along the longitudinal direction and the central electrode surrounds the aperture in a lateral plane perpendicular to the longitudinal direction to define a transverse cavity for trapping charged particles.

A portable, stand-alone microfluidic interrogation device including a microprocessor and a touch-screen display. The touch-screen display can receive one or more user input to select a particular particle interrogation procedure, and subsequently show interrogation results. A microfluidic path extending through the interrogation device includes alignment structure that defines an interrogation zone in which particles carried in a fluid are urged toward single-file travel. Operable alignment structure may define sheath-, or non-sheath fluid flow. Desirably, a portion of the alignment structure is removable from the device in a tool-free procedure. The device may operate under the Coulter principle, and/or detect Stokes' shift phenomena, and/or other optically-based signal(s).

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionization source, a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. A first vacuum pump is arranged and adapted to pump the first vacuum chamber, wherein the first vacuum pump is arranged and adapted to maintain the first vacuum chamber at a pressure <10 mbar. A first RF ion guide is located within the first vacuum chamber. An ion detector is located in the third vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is 400 mm. The mass spectrometer further comprises a split flow turbomolecular vacuum pump comprising an intermediate or interstage port connected to the second vacuum chamber and a high vacuum (HV) port connected to the third vacuum chamber. The first vacuum pump is also arranged and adapted to act as a backing vacuum pump to the split flow turbomolecular vacuum pump. The first vacuum pump has a maximum pumping speed 10 m.sup.3/hr (2.78 L/s).

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionisation source 701, a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. A first vacuum pump 707 is arranged and adapted to pump the first vacuum chamber, wherein the first vacuum pump 707 is arranged and adapted to maintain the first vacuum chamber at a pressure <10 mbar. A first RF ion guide 702 is located within the first vacuum chamber. An ion detector 705 is located in the third vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector 705 is 400 mm. The mass spectrometer further comprises a split flow turbomolecular vacuum pump 706 comprising an intermediate or interstage port connected to the second vacuum chamber and a high vacuum (HV) port connected to the third vacuum chamber. The first vacuum pump 707 is also arranged and adapted to act as a backing vacuum pump to the split flow turbomolecular vacuum pump 706. The first vacuum pump has a maximum pumping speed 10 m.sup.3/hr (2.78 L/s).

A mass spectrometer system can include an ion source, a vacuum chamber; a mass analyzer within the vacuum chamber, a transfer tube between the ion source and the vacuum chamber, a transfer tube heater, and a vacuum pump. The mass spectrometer system can be configured to reduce the pump speed of the vacuum pump in response to receiving a transfer tube swap instruction; lower the temperature of the transfer tube to below a first threshold; operating the vacuum pump at the reduced pump speed while the transfer tube is replaced with a second transfer tube; heating the second transfer tube to a temperature above a pump down temperature; and increasing the pump speed of the vacuum pump after the temperature of the second transfer tube exceeds a second threshold.

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionization source, a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. A first vacuum pump is arranged and adapted to pump the first vacuum chamber, wherein the first vacuum pump is arranged and adapted to maintain the first vacuum chamber at a pressure <10 mbar. A first RF ion guide is located within the first vacuum chamber and an ion detector is located in the third vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is 400 mm. The mass spectrometer further comprises a tandem quadrupole mass analyzer, a 3D ion trap mass analyzer, a 2D or linear ion trap mass analyzer, a Time of Flight mass analyzer, a quadrupole-Time of Flight mass analyzer or an electrostatic mass analyzer arranged in the third vacuum chamber. A split flow turbomolecular vacuum pump comprising an intermediate or interstage port is connected to the second vacuum chamber and a high vacuum (HV) port is connected to the third vacuum chamber. The first vacuum pump is also arranged and adapted to act as a backing vacuum pump to the split flow turbomolecular vacuum pump and the first vacuum pump has a maximum pumping speed 10 m.sup.3/hr (2.78 L/s).

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionisation source, a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. An ion detector is located in the third vacuum chamber. A first RF ion guide is located within the first vacuum chamber and a second RF ion guide is located within the second vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is 400 mm. The product of the pressure P.sub.1 in the vicinity of the first RF ion guide and the length L.sub.1 of the first RF ion guide is in the range 10-100 mbar-cm and the product of the pressure P.sub.2 in the vicinity of the second RF ion guide and the length L.sub.2 of the second RF ion guide is in the range 0.05-0.3 mbar-cm.

An ion transfer device that includes first and second ion guides, one or more RF voltage sources, and one or more DC voltage sources that produce first and second potential gradients in the first and second ion guides such that the potential gradients push the ions to move toward the outlet of each ion guide such that the one or more DC voltages produce a potential barrier, the potential barrier preventing the ions in the first ion guide from entering the second ion guide, the ions accumulate behind the potential barrier and form an ion packet, and the potential barrier is reduced after a predetermined time period to move the ion packet from the first ion guide to the second ion guide.

An impedance-matched coaxial conductor for a vacuum environment, comprising an electrically conducting inner conductor, an electrically conducting outer hollow conductor configured to surround the inner conductor substantially along its entire length, whereby the outer hollow conductor is separated from the inner conductor, at least an electrically isolating element positioned between the inner conductor and the outer hollow conductor in order to maintain the separation between them, a space between the inner conductor and the outer hollow conductor being vacuum pumpable. An electrically conducting contacting element for a vacuum environment, which is configured to establish an electrical contact between a first conductor and a second conductor, comprising a body made from an electrically conducting material; at least a through hole in the body, configured to accept inside the hole the first conductor in form of an elongated electrical conductor; at least a first threaded hole in the body, oriented substantially perpendicular to the through hole, and extending from an outside surface of the body to the through hole, the threaded hole being configured to accept a screw; and at least a second threaded hole in the body. A time-of-flight mass analyzer comprising a plurality of functional parts selected from at least the following list: an ion source, an extraction region, a drift region, a reflectron, and a detector; a single vacuum flange configured to connect on a vacuum chamber; a plurality of platforms; at least one pillar for each of the plurality of platforms, configured for fixing and distancing the corresponding platform either to the single vacuum flange or to a neighboring platform from the plurality of platforms; each of the plurality of platforms being configured to gather a subset of the plurality of functional parts to obtain a subassembly; and the subassemblies and the single vacuum flange being arranged to form a longish elongated assembly in which each of the platforms defines a mechanical reference in the longish elongated assembly.

The invention generally relates to sample analysis systems and methods of use thereof. In certain aspects, the invention provides a system for analyzing a sample that includes an ion generator configured to generate ions from a sample. The system additionally includes an ion separator configured to separate at or above atmospheric pressure the ions received from the ion generator without use of laminar flowing gas, and a detector that receives and detects the separated ions.