Provided herein are improved methods for preparing phospholipid formulations including phospholipid UCA formulations.

A coupling agent composition is provided and includes a plurality of non-toxic components in an amount equal to or greater than 97 weight percent (wt %). The coupling agent composition includes a carrier of water, at least one water soluble polyol selected from the group consisting of propanediol, pentylene glycol, and butylene glycol, a thickening agent having a naturally derived soluble polymer additive selected from a group consisting of xanthan gum, carrageenan, guar, locust bean, sodium hyaluronate, sodium alginate, acacia, tara gum, tamarind seed, succinoglycan, scleroglucan, and mannan, and a balance of incidental impurities.

Microresonator structures including a top polymer film layer, a bottom polymer film layer, and a smart hydrogel structure sandwiched between the polymer film layers. An ultrasound resonator cavity having a resonance frequency is defined between the top and bottom polymer layers, and the smart hydrogel structure is configured to provide a change in height to the ultrasound resonator cavity due to volumetric expansion or contraction of the smart hydrogel structure, in response to interaction of the smart hydrogel structure with one or more predefined analytes in an in vivo or other environment. Related methods of use for determining the presence or concentration of a given target analyte, as well as methods of fabricating such microresonator structures are also described.

Provided herein are improved methods for preparing phospholipid formulations including phospholipid UCA formulations.

Provided herein are improved methods for preparing phospholipid formulations including phospholipid UCA formulations.

Provided herein are improved methods for preparing phospholipid formulations including phospholipid UCA formulations.

A method and system are used to enhance a marker material to include a plurality of air bubbles. The method of manufacturing a marker includes enhancing a marker material to include a plurality of air bubbles using at least a first EFD and a second EFD. The method may include cycling repeatedly through a transfer process between a first container and a second container. A system for enhancing a marker material includes a transfer apparatus configured to receive a marker material and a selected amount of air. The system comprises a first EFD coupled to a first end of the transfer apparatus and a second EFD coupled to a second end of the transfer apparatus.

Disclosed is a suspension of gas-filled microbubbles in a physiologically acceptable liquid carrier comprising a lipid mixture of a first lipid having transition temperature of about 41° C. such as DPPC or DPPG, a second lipid having transition temperature of about 55° C. such as DSPC or DSPG, and a PEGylated DSPE such as DSPE-PEG2000, DSPE-PEG3000, or DSPE-PEG5000, and methods of preparation thereof.

Systems and methods for accurately measuring changes in biomarker sensitive hydrogel volume and shape due to exposure to various biomarkers include a system for identifying one or more dimensional changes in a biomarker sensitive hydrogel positioned within an in vivo environment. The system includes a biomarker sensitive hydrogel positioned within an in vivo environment and configured to dimensionally change in response to interaction with predefined biomarkers. The system additionally includes an ultrasound transducer for locating and identifying one or more characteristics of the biomarker sensitive hydrogel and a computer system in electrical communication with the ultrasound transducer. The computer system is configured to receive characteristics of the biomarker sensitive hydrogel from the ultrasound transducer and determine dimensional changes of the biomarker sensitive hydrogel based on the received characteristics.

There is described herein a nanoparticle comprising an outer shell comprising a porphyrin salt, an expanded porphyrin salt or an analog of porphyrin salt, around an inner oil core.