A method for preparing a multifunctional hydrogel by yeast fermentation is provided. According to the present disclosure, graphene oxide is reduced by polydopamine to obtain a reduced graphene oxide solution first, and then a certain concentration of a gelatin-PCA-glucose mixed solution and an activated yeast liquid are prepared. The three solutions are uniformly mixed and stirred by a one pot reaction method, poured into a mold, and subjected to fermentation in a 30° C. water bath for a certain period of time, so as to obtain a Gel-PrGO-PCA-yeast multifunctional hydrogel material. The method is simple, convenient, rapid, and efficient. The obtained hydrogel has good air permeability, superior mechanical properties, electrical conductivity, and biocompatibility, and can be applied to different skin locations to achieve electrocardiograph and electromyographic detection.

A biodegradable and biocompatible three dimensional construct comprising a combination of a nano silicate (e.g., laponite) and two different polymers, the two polymers each individually providing at least one covalently linked polymer chain and at least one ionically linked polymer chain, the polymeric chains forming a dual strengthening intertwined polymeric system. The constructs demonstrate improved mechanical and strength properties, while the bioinks provide a material having superior printability characteristics suitable for printing a three dimensional biodegradable construct having an aspect ratio of greater than 2.0. The bioink may also comprise cells or combinations of cells. Methods of using the constructs and bioinks for wound healing preparations and tissue regeneration are also provided.

The present disclosure relates to a method for preparing an absorbable haemostatic composition for the body and the haemostatic composition prepared thereby, and the present disclosure is for providing a method for preparing an absorbable haemostatic composition for the body and the haemostatic composition prepared thereby, wherein the haemostatic composition can be used directly on the wound site when bleeding occurs in the surgical area such as surgical operation, trauma, etc. so that hemostasis can be effectively performed, the haemostatic composition can perform wound sealing, tissue repairing promotion, wound surface tissue protection, infection prevention, etc., the haemostatic composition is contained in haemostatic products such as gauze (cotton yarn), sponge, etc. to accelerate the hemostasis speed of the bleeding site and enable rapid hemostasis to be able to shorten the hemostasis time at the same time.

The present invention addresses the problem of providing a powder that has excellent adhesive strength to biological tissue when applied to a wound-covering material or the like. The present invention also addresses the problem of providing a wound-covering material and an adhesion prevention material. The powder contains particles containing a crosslinked gelatin derivative, wherein the gelatin derivative has a structure represented by formula (1): GltnNH-L-CHR.sup.1R.sup.2 (in the formula, Gltn represents a residue of gelatin, L represents a single bond or a divalent linking group, R.sup.1 represents a hydrocarbon group having 1-20 carbon atoms, and R.sup.2 represents a hydrogen atom or a hydrocarbon group having 1-20 carbon atoms), the particles have an average sphericity of 1.45 or less, and the standard deviation of the sphericity of the particles is 0.25 or less.

A preparation method of calcium peroxide-mediated in situ crosslinkable hydrogel as a sustained oxygen-generating matrix, includes: a) reacting a natural or a synthetic polymer with Traut's reagent (TR) in a solvent, and synthesizing a polymer derivative having thiol group in backbone of the polymer derivative; and b) mixing and reacting a solution of the polymer derivative having thiol group with calcium peroxide (CaO.sub.2), and thereby forming a hydrogel, wherein in the step b), disulfide bonds (—S—S) are induced between backbones of the polymer derivative having thiol group attached by decomposition of calcium peroxide (CaO.sub.2), and thereby in situ crosslinking is formed.

According to an illustrative embodiment a method to promote healing of a wound is provided comprising contacting the wound with a biologically active composition comprising a lipoic acid derivative and gelatin. In another embodiment a wound dressing is provided comprising a scaffold coated with a biologically active composition comprising a lipoic acid derivative. In a further embodiment, a system is provided for treating a tissue site of a patient, the system comprising a reduced-pressure source to supply reduced pressure, a manifold to distribute reduced pressure to a tissue site and a scaffold coated with a biologically active composition comprising a lipoic acid derivative. Methods for producing such a system and scaffold are also disclosed.

Disclosed is a dry composition comprising one or more polyols, which upon addition of an aqueous medium forms a substantially homogenous paste suitable for use in haemostasis procedures. The paste reconstitutes spontaneously upon addition of the liquid; hence no mechanical mixing is required for said paste to form. The composition may further comprise an extrusion enhancer, such as albumin. Also disclosed are methods of preparing said dry composition, a paste obtained from said dry composition and uses of said dry composition or paste for medical and surgical purposes.

One aspect of the invention provides a method of sequentially inducing macrophage conversion in a wound. The method includes: (a) administering IL-4 to induce conversion of a first population of wound macrophages in the wound to M2A macrophages; and then (b) administering IL-10, dexamethasone, or a dexamethasone analog to induce conversion of a second population of wound macrophages in the wound to M2C macrophages.

According to an illustrative embodiment a method to promote healing of a wound is provided comprising contacting the wound with a biologically active composition comprising a lipoic acid derivative and gelatin. In another embodiment a wound dressing is provided comprising a scaffold coated with a biologically active composition comprising a lipoic acid derivative. In a further embodiment, a system is provided for treating a tissue site of a patient, the system comprising a reduced-pressure source to supply reduced pressure, a manifold to distribute reduced pressure to a tissue site and a scaffold coated with a biologically active composition comprising a lipoic acid derivative. Methods for producing such a system and scaffold are also disclosed.

The present invention is directed to a hemostatic semi-solid paste material comprising a bioabsorbable carrier hemostatic material; and a supplemental hemostatic agent; wherein the paste material has an elongated form extending along a lengthwise axis with an aspect ratio of at least 3; wherein the paste is self-supporting and syringe extrudable; and wherein the supplemental hemostatic agent has a non-homogenous distribution profile across a cross-section taken across the lengthwise axis. In another aspect, the present invention relates to devices for forming and expressing the hemostatic material.