The present disclosure discloses a xeno-free bio-ink formulation amenable to be printed using a 3D printer. The bio-ink formulation exhibits optimum viscosity in the range of 1690-5300 cP. The present disclosure discloses a bio-printed corneal lenticule obtained from the bio-ink formulation. The bio-printed corneal lenticule as disclosed is of the optimum thickness in the range of 10-500 microns and exhibits transmittance in the range of 80-99%. The present disclosure also discloses a process for preparing the bio-ink formulation as well as for preparing the bio-printed corneal lenticule. Further, the present disclosure discloses a method of treating a corneal defect using the bio-printed corneal lenticule as an implant to treat the corneal defect. The bio-printed corneal lenticule can further be used as a model for in-vitro drug testing and diseases modelling.

Compositions and methods for the promotion of wound healing and tissue regeneration are described. The compositions and methods make use of water-soluble soy protein isolates (WSsoy), Fraction 5, Fraction 9, and/or bioactive peptide components of soy protein isolates. The invention also relates to the unexpected discovery that purified WSsoy forms gel-like matrices when suspended within certain concentration ranges in an aqueous environment. The compositions of the invention comprising WSsoy promote natural healing and have a low risk profile.

There is described a multiphasic osteochondral scaffold for osteochondral defect repair, the scaffold comprising a bone phase and a cartilage phase, wherein the bone phase comprises a support matrix and the cartilage phase comprises a polymeric matrix, and the scaffold comprises a non-porous layer between the bone phase and the cartilage phase. Also described is a multiphasic osteochondral scaffold for osteochondral defect repair, the scaffold comprising a bone phase and a cartilage phase, wherein the bone phase comprises a support matrix and the cartilage phase comprises a polymeric matrix, and wherein the support matrix is tapered so that the dimensions of the support matrix are less at the lower end of the support matrix than at the upper end of the support matrix.

Coated and expanded, nanofiber structures are provided and methods of use thereof.

A biodegradable and biocompatible three dimensional construct comprising a combination of a nano silicate (e.g., laponite) and two different polymers, the two polymers each individually providing at least one covalently linked polymer chain and at least one ionically linked polymer chain, the polymeric chains forming a dual strengthening intertwined polymeric system. The constructs demonstrate improved mechanical and strength properties, while the bioinks provide a material having superior printability characteristics suitable for printing a three dimensional biodegradable construct having an aspect ratio of greater than 2.0. The bioink may also comprise cells or combinations of cells. Methods of using the constructs and bioinks for wound healing preparations and tissue regeneration are also provided.

The present invention relates to a bellows framework having a concave-convex structure on at least one of outer and inner sides using three-dimensional printing technology and a method of producing thereof, and an artificial tracheal replacement comprising an epithelium part formed on the inner side of the bellows framework and an annular cartilage part formed along the circumference of concave-convex grooves on the outer side and a method of producing thereof.

A malleable demineralized bone composition consists of cortical bone made from a first portion and a second portion. The first portion and second portion of cortical bone is made from cut pieces freeze dried then ground into particles and demineralized then freeze-dried. A volume of the second portion is placed in a solution of sterile water to create a mixture, the water volume being seven times the volume of the second portion, the mixture is autoclaved under heat and pressure to form a gelatin, and the first portion is mixed with the gelatin to form a malleable putty or paste.

The present disclosure discloses embodiments of a formulation for application to the cornea, the formulation comprising a hyaluronic acid, a gelatin, and exosomes. In certain variations, the exosomes may be naive mesenchymal stem cell-derived exosomes, primed mesenchymal stem cell derived-exosomes, or corneal stromal stem cell derived-exosomes. In certain variations, the primed mesenchymal stem cell-derived exosomes are exosomes derived from mesenchymal stem cells primed with a corneal stromal stem cell derived-conditioned medium.

Described herein are apparatuses, systems, and methods for fabricating tissue constructs, such as by fabricating perfusable tissue constructs by embedding a sacrificial material into a composite matrix yield stress support bath. A composite matrix bath can include a microgel filler and a hydrogel precursor. An extrusion tip can be used for embedded printing of perfusable tissue constructs by disposing sacrificial material into the composite matrix bath while the extrusion tip travels along a predefined course through the composite matrix bath. This sacrificial material can be the printed tissue construct or can be removed to render the matrix bath a perfusable tissue construct. The composite matrix bath can include acellular or cell-laden hydrogels. The sacrificial material can include a salt and a physiological buffer or a non-cytotoxic porogen material. The hydrogel precursor can include at least one of gellan and gelatin. Cross-linking can be carried out chemically, thermally, enzymatically, or physically.

An object of the present invention is to provide a bone regenerative agent showing a high bone regeneration effect. According to the present invention, a bone regenerative agent containing gelatin-containing granules and stem cells is provided. Gelatin preferably has repetitions of a sequence represented by Gly-X-Y, which is characteristic of collagen. Here, a plurality of Gly-X-Y's may be the same as or different from each other, and in the formula, X and Y each independently represent any of amino acid. A molecular weight of the gelatin is preferably 2 KDa or more and 100 KDa or less.