The invention relates to methods for producing a polymeric scaffold for use in tissue engineering applications or soft tissue surgery, as well as to the produced scaffolds and an associated kit. The method features a first fast drying step of applying a mechanical compression on a polymeric gel layer and a second slow drying step of the gel up to reach a polymer mass fraction of at least 60% w/w in the final scaffold. The method allows the production of scaffolds with high regeneration and healing properties of a grafted tissue via host cell invasion and colonization, and a good suturability. These goals are achieved through the formation within the scaffold of a non-uniform architecture creating softer and stiffer areas, which is maintained even upon re-swelling of the scaffold upon hydration of the final dried product.

A preparation method of calcium peroxide-mediated in situ crosslinkable hydrogel as a sustained oxygen-generating matrix, includes: a) reacting a natural or a synthetic polymer with Traut's reagent (TR) in a solvent, and synthesizing a polymer derivative having thiol group in backbone of the polymer derivative; and b) mixing and reacting a solution of the polymer derivative having thiol group with calcium peroxide (CaO.sub.2), and thereby forming a hydrogel, wherein in the step b), disulfide bonds (—S—S) are induced between backbones of the polymer derivative having thiol group attached by decomposition of calcium peroxide (CaO.sub.2), and thereby in situ crosslinking is formed.

The invention relates to medical biotechnology, medicine, traumatology, orthopedics, dentistry, and orthodontics. A method is proposed for producing a biological matrix intended for the repairing of bone tissue defects; said method may include a plurality of consecutive stages, such as pre-treating biological material, coarse filtering and fractionating, fine filtering and extracting, delipidizing, fermenting, demineralizing, and sterilizing in supercritical fluid. The resulting bioresorbable biological matrix is characterized by increased osteo- and biointegration, an optimal biodegradation rate, high biocompatibility, an absence of recipient immunoreactivity, high osteoconduction capacity, and pronounced osteogenic potential in osteosynthesis and bone grafting. The matrix consists of ossein, hydroxyapatite and/or calcium phosphate, wherein the ossein is in native unreduced form with its three-dimensional structure completely intact, the hydroxyapatite and calcium phosphate are in native amorphous form, and the matrix itself, from which cellular debris, foreign lipids, nucleic acids, and immunogens have been removed, contains residual amounts of bone morphogenetic proteins and is impregnated with vesicular phosphatidylcholine and/or cholesterol containing gelatin (hydrolyzed collagen), and/or bone atelocollagen, and/or poly-(ε-caprolactone) and additionally containing biologically active substances (including bioactive peptides and growth factors).

The present invention relates to a biomaterial comprising adipose-derived stem cells (ASCs), an extracellular matrix and gelatin. The present invention also relates to methods for producing the biomaterial and uses thereof.

The present invention discloses compositions and methods for repair and reconstruction of defects and injuries to soft tissues. Some aspects of the disclosure provide methods for corneal reconstruction by applying an engineered bioadhesive, glycidyl methacrylate-substituted gelatin and a visible light activated photoinitiator in presence of visible light to the corneal defect.

The present disclosure is concerned with magneto-patterned cell-laden hydrogel materials and methods of making and using those materials. The disclosed materials are useful for, among other things, repair of tissue defects, e.g., tissue at a tissue interface such as a bone-cartilage interface.

The invention features biodegradable materials, and in vitro and in vivo methods of using such compositions to promote bone and soft tissue growth and healing.

The present invention provides a method for functionalizing a medical implant surface to promote osseointegration upon implantation in bone tissue.

Disclosed is a degradable sponge block with an infiltrating stem cell suspension, and the stem cells are filled in the network structure holes of the sponge material in a cell suspension state. The stem cell preparation sponge patch complex can be clamped by the instrument and directly delivered to the physiological site of cell therapy due to its rigid support. In addition, the complex is also capable of deformation, which can be fixed at the healing site and realize in-situ release of cells. The cells are loosely bound to the sponge, which can realize the cell preparation reaching the treatment site under the carrying of sponge materials, and release the cell preparation under the action of external forces. The sponge materials only play the role of loading stem cells and carrying them to the treatment site, and then biodegradation and avoiding foreign body residue.

The technology relates to a 3D printed hydrogel formed from a maleimide containing polymer cross-linked using a bis-thiol containing cross-linking agent having at least two thiol functional groups, processes for preparing the 3D printed hydrogel, and uses thereof.