Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.

The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

Provided subject matter relates to tissue engineering. More specifically provided are kits, devices and methods for in situ repair and regeneration of guided and functional growth of cells and cell components by providing into the injury site biomaterial solution including the cell(s), magnetic particles and solidifying the biomaterial while applying the magnetic field.

Provided subject matter relates to tissue engineering. More specifically provided are kits, devices and methods for in situ repair and regeneration of guided and functional growth of cells and cell components by providing into the injury site biomaterial solution including the cell(s), magnetic particles and solidifying the biomaterial while applying the magnetic field.

A method of preparing a crosslinked, collagen-based wound care dressing is provided, comprising: (a) immersing a sample of fibrous and/or non-fibrous collagen in a buffered acidic, aqueous solution comprising an alcohol; (b) contacting the collagen in solution with a catalytic component comprising 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride for a time at least sufficient to effect reaction between amino and carboxyl groups present on the collagen and to yield crosslinked collagen that is resistant to pronase degradation; and (c) drying the crosslinked collagen to yield a porous, crosslinked collagen article wherein the porous, crosslinked collagen article demonstrates a pore size of 10-500 microns. Also provided are bioactive collagen medical scaffolds for hernia repair prosthetics and surgical incision closure members, prepared using the method above.

A method of preparing a crosslinked, collagen-based wound care dressing is provided, comprising: (a) immersing a sample of fibrous and/or non-fibrous collagen in a buffered acidic, aqueous solution comprising an alcohol; (b) contacting the collagen in solution with a catalytic component comprising 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride for a time at least sufficient to effect reaction between amino and carboxyl groups present on the collagen and to yield crosslinked collagen that is resistant to pronase degradation; and (c) drying the crosslinked collagen to yield a porous, crosslinked collagen article wherein the porous, crosslinked collagen article demonstrates a pore size of 10-500 microns. Also provided are bioactive collagen medical scaffolds for hernia repair prosthetics and surgical incision closure members, prepared using the method above.

A composite scaffold having a highly porous interior with increased surface area and void volume is surrounded by a flexible support structure that substantially maintains its three-dimensional shape under tension and provides mechanical reinforcement during repair or reconstruction of soft tissue while simultaneously facilitating regeneration of functional tissue.