The present invention relates to the field of treatment of bone defects. The invention provides a composition for treatment of bone defects as well as methods for treatment of such defects.

A method of making an electron beam irradiated osteoinductive implant is provided. The method comprises exposing an osteoinductive implant containing demineralized bone matrix (DBM) fibers to electron beam radiation at a dose of from about 10 kilograys to 100 kilograys for a period of time. The electron beam irradiation reduces microorganisms in the osteoinductive implant, and the electron beam irradiated osteoinductive implant retains osteoinductive properties. Methods of implantation and an irradiated osteoinductive implant are also disclosed.

A composition includes a dual crosslinkable hydrogel that includes a plurality of polymer macromers, which are crosslinked with a first agent and a second agent different than the first agent, wherein the crosslinks formed using the second agent are reversible and repeatable to allow the mechanical properties of the hydrogel to be dynamically adjusted.

Embodiments of bony region stabilization and fusion constructs are described generally herein including bony fixation elements including porous, bone fusion enabling or promoting regions or osteo conductive enabling or promoting regions. Other embodiments may be described and claimed.

A method of preparing a reconstitutable implantable bone putty includes combining a bone matrix derived from human bone and gelatin particulates derived from human tissue at a concentration of the bone matrix by dry weight of 20 to 60 percent to form the reconstitutable implantable bone putty. Preparing the gelatin particulates includes supplying a gelatin precursor of bone or soft tissue from a human, treating the gelatin precursor with phosphoric acid to generate a gelatin-acid mixture, neutralizing the gelatin-acid mixture with an alkali to a pH between 6 and 8 to allow a gelatin-rich solution and a waste solution to separate, removing residual salts from the gelatin-rich solution to obtain purified gelatin, drying the purified gelatin, and reducing the purified gelatin to particulates having a largest dimension less than 300 μm. A method of preparing an implantable bone putty includes adding a reconstitution media to the reconstitutable implantable bone putty.

The invention provides a method of making a biological disc graft. In one embodiment, the biological disc graft is useful for treating back or neck pain. In one embodiment, the biological disc graft is useful for treating any joint pain. The invention also provides a method of implanting said biological disc graft in a way that is minimally invasive and less dangerous.

Provided is an injectable implant configured to fit at or near a bone defect to promote bone growth, the injectable implant comprising lyophilized demineralized bone matrix (DBM) being in fiber and particle forms; alginate; and a liquid carrier, wherein the DBM is in an amount of about 20 wt. % to about 40 wt. % of a total weight of the injectable implant, the alginate is in an amount of from about 3 wt. % to about 10 wt. % of the total weight of the injectable implant, and the liquid carrier is in an amount from about 50 wt. % to about 70 wt. % of the total weight of the injectable implant. A moldable implant and methods of making the implants are further provided.

The invention provides acellular matrix from mammalian teeth for preparing a safe graft or bone replacement in regenerative medicine.

A method for instant lumbar spine fusion between two vertebrae in a patient includes establishing under X-ray fluoroscopy the location of the transpedicular notch of the next lower vertebra in caudal direction, making a percutaneous incision to the transpedicular notch, inserting a cannulated guide, drilling a transpedicular approach from the pedicle of the lower vertebra to the anterior part of the vertebral body of the vertebrae above the disc to be treated, inserting a working cannula through the previously drilled approach reaching the intervertebral disk, cleaning and scrapping the intervertebral disk space, inserting transpedicularly at least one intervertebral stabilizing screw, and acting on both intervertebral screws with screwdrivers in order to distract or contract both screws allowing to adjust or correct the intervertebral distance of the disk. The method can be performed on an outpatient basis.

The present patent application is directed to compositions and shaped structures implantable into mammalian bodies, the compositions and shaped structures having localized bioactive surfaces.