In one aspect, an implant for replacing subject tissue includes a nonbiologic portion and a biologic portion grown on the nonbiologic portion. The biologic portion may be grown on the nonbiologic portion before being implanted in the subject. The nonbiologic portion may comprise a porous metal substrate (e.g., scaffolding). The nonbiologic portion may be formed by 3D printing (i.e., additive manufacturing). The nonbiologic portion may be patient-specific. A robot may be used to shape the implant before implantation and/or to shape bone being replaced/resurfaced.

The invention is directed towards a process for implanting a cartilage graft into a cartilage defect and sealing the implanted cartilage graft with recipient tissue. The invention is also directed towards a process for repairing a cartilage defect and implanting a cartilage graft into a human or animal. The invention is further directed toward a repaired cartilage defect.

Disclosed herein is a method of producing collagen particles. Each of the collagen particle is characterized in having a particle size of about 10-250 m, in which the integrity of collagen fibers therein is relatively intact.

This invention provides porated cartilage products, methods of producing porated cartilage products, and methods of treating subjects by administering cartilage products. Optionally, the cartilage products are sized, porated, and digested to provide a flexible cartilage product. Optionally, the cartilage products comprise viable chondrocytes, bioactive factors such as chondrogenic factors, and a collagen type II matrix. Optionally, the cartilage products are non-immunogenic.

A process and system provides for atraumatic preparation of morselized Tissue Particles (TP)s, such as Full Thickness Skin Graft Particles (FTSGPs), cartilage particles and other organ tissue particles, in a liquid medium. The resultant tissue product may be a suspension of Tissue Particles in an aqueous solution and containing highly viable cells and may be rapidly prepared at bedside or in the operating room and conveniently delivered to a patient through a syringe or similar applicator. The morselized Tissues Particles may be used for surgical applications including wound healing, cosmetic surgery, and orthopedic cartilage repairs.

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to TMJ implantation materials and implants (e.g., temporomandi bular joint (TMJ) methods of making TMJ implantation materials and implants, methods of forming a TMJ implantation material or an implant, and the like.

The present invention provides a method of preparing biomembrane, closed structure with biomembrane characteristics or cellular compartment, comprising the following steps: 1), acquire biological cells from natural tissues or natural biological species; 2), culture the cells obtained in step 1) massively in an appropriate environment; 3), acquire the lysates of cells in step 2), and extracting the biomembrane, closed structure with biomembrane characteristics and cellular compartment through differential centrifugation, density gradient centrifugation or dual-phase extraction individually or a combination of two methods or a combination of three methods thereof. The membrane is a natural biomembrane, closed structure with biomembrane characteristics and cellular compartment, which can be used for package of active ingredients in various fields.

There are disclosed compositions for achieving reverse phase characteristics, methods of preparation thereof, and the use of amniotic tissue for cartilage repair. In an embodiment, a biocompatible articular tissue repair composition may have a therapeutic material and a carrier configured for achieving reverse phase characteristics, and methods for using the composition. In various embodiments, the therapeutic material may be amniotic tissue. In various embodiments, the carrier may be a poloxamer such as poloxamer 407. Other embodiments are also disclosed.

The invention relates to a graft for repairing articular cartilage defects, comprising at least one of an autologous costal cartilage or an allogeneic costal cartilage, wherein the graft can be a whole piece of costal cartilage or a cartilage particle-bound hydrogel graft. The invention further relates to the use of the graft and a method for repairing articular cartilage defects. In the present invention, it has a small secondary injury by using costal cartilage implantation. In addition, it can be a minimally invasive operation, avoiding the risk of complications such as prosthesis loosening and infection due to the artificial joint replacement. Because the amount of costal cartilage is sufficient, it can meet the needs of multiple cartilage reconstruction and revision surgeries. Individualized reconstruction of injured articular cartilage surface can be achieved, which provides a safer, more operable repair method for patients diagnosed with articular cartilage defects.

Disclosed is a method for preparing a cell growth scaffold having a structural memory feature, comprising a step of preparing a micro-fibrous or flocculent acellular tissue matrix material; a step of preparing an acidification-treated hydrogel-like acellular tissue matrix particles; proportionally mixing the micro-fibrous or flocculent acellular tissue matrix material with the acidification-treated hydrogel-like acellular tissue matrix particles, followed by injection-molding, freezing treatment, radiation treatment, and ultimately preparing a porous cell growth scaffold that can be stored at room temperature. The prepared cell growth scaffold is a porous cell growth scaffold that has no chemical crosslinking, and has a biological activity, a stable three-dimensional structure and a structural memory feature. The cell growth scaffold has an excellent biocompatibility and complete biodegradability, and supports the growth of cells and the growth of tissues and organs in vitro and in vivo, thereby being suitable for repair of human soft tissue traumas and defects.