Embodiments of the present disclosure encompass systems and methods for in-situ/in vivo, bottom-up tissue generation for wound repair, repair of tissue defects, and the like. Embodiments of the systems of the present disclosure include modular scaffolds seeded with cells (modular tissue forming units (MTFUs)) for packing a tissue defect, such that these MTFUs are able to fill the wound bed with cells of one or more needed tissue types supported by the modular scaffolding particles.

In particular, a composition for breast augmentation that includes a composition promoting an increase in subcutaneous tissue, wherein the promoting composition aims at accumulating and increasing adipose tissue under the skin of a breast by generating and increasing the adipose tissue around a mammary gland and enables recovery of autologous tissue and recovery of appearance by a safe and natural process, and a method for breast augmentation are provided. A composition promoting an increase in subcutaneous tissue that contains autologous plasma and a basic fibroblast growth factor (b-FGF), in particular, a composition for breast augmentation is provided. A composition for breast augmentation that includes the above-described composition promoting an increase in subcutaneous tissue that further contains fat is provided. Specifically, a composition for breast augmentation that includes the above-described composition promoting an increase in subcutaneous tissue, wherein the fat is in the form of a lipid emulsion and the lipid emulsion is an oil-in-water lipid emulsion produced by emulsifying fat and oil in the presence of an emulsifier, and a method for breast augmentation by using the composition are provided. An injection unit of the composition for breast augmentation is also provided.

A support for use with tissue or bone is described, the support having one or more spaced apart brace members, and at least one elongated linking member for linking to the one or more spaced apart brace members. The support may be provided in use in combination with a biologic glue, the biologic glue comprising a first portion containing stabilized blood product, and a second portion containing a growth factor, the first portion and the second portion comingled when or after the support is introduced to the tissue or bone in a subject in need thereof. The support is fabricated by actively producing the support through an additive manufacturing process, in which the support is customized specifically to the tissue or bone by creating a computer-generated construct of the support on a three-dimensional volume rendering of the tissue or bone, and the computer generated construct is interpretable via software directing the additive manufacturing process.

A medical implant packaging assembly having an outer tray, an inner tray and a retainer. An implant which may include a hydrated tissue graft may be positioned within the packaging assembly. The inner tray is formed from a moisture barrier material whereby the implant may be maintained in a hydrated state, even if the implant is not immersed in fluid within the packaging assembly, thereby avoiding need for rehydrating steps that are required for dehydrated and/or cryopreserved implants prior to or during surgery. The outer tray and inner tray and a retainer are also made of transparent materials, enabling medical personnel to view the implant through these packaging components prior to and during surgery.

Disclosed is a method for preparing a cell growth scaffold having a structural memory feature, comprising a step of preparing a micro-fibrous or flocculent acellular tissue matrix material; a step of preparing an acidification-treated hydrogel-like acellular tissue matrix particles; proportionally mixing the micro-fibrous or flocculent acellular tissue matrix material with the acidification-treated hydrogel-like acellular tissue matrix particles, followed by injection-molding, freezing treatment, radiation treatment, and ultimately preparing a porous cell growth scaffold that can be stored at room temperature. The prepared cell growth scaffold is a porous cell growth scaffold that has no chemical crosslinking, and has a biological activity, a stable three-dimensional structure and a structural memory feature. The cell growth scaffold has an excellent biocompatibility and complete biodegradability, and supports the growth of cells and the growth of tissues and organs in vitro and in vivo, thereby being suitable for repair of human soft tissue traumas and defects.

The present invention relates to an in vitro method for creating a viable connective tissue and/or osseous tissue obtained by tribological solicitations of a biological culture. It further relates to a viable connective tissue and/or osseous tissue susceptible to be obtained by said method as well as to the use of said method or viable connective tissue and/or osseous tissue to prepare a biological implant.

Methods of implanting therapeutic cells in a subject and methods of preparing pancreatic islet cells for implantation into a subject, prior to implantation into a subject, are provided herein.

The present disclosure relates to bioactive hydrogels derived from human blood plasma. More particularly, the disclosure relates to multifunctional materials for cell encapsulation, cell culture platforms, medical treatment apparatus and methods, more particularly, hydrogels derived from human blood components and technologies for use of such materials in research, biomedical treatment, biotech and pharmaceutical industry. The disclosure further relates to 3D printable scaffolds, sponges, foams, fibers, particles, capsules, membranes and injectable systems comprising said hydrogel. Additionally, this disclosure allows for the controlled placement of biologically active components that may be delivered by the hydrogel compositions.

The present disclosure describes methods of treating a ligament injury with ligament grafting.

Methods are provided for preparing liquid PRF and using the liquid PRF as a drug-delivery carrier system for other regenerative biomaterials and biomolecules. This carrier system provides more effective regenerative strategies for improved tissue wound healing, treatment, and regeneration in the body by supplying additional autologous growth factors into commonly-utilized biomaterials improving the host-tissue response to such therapies.