A bioscaffold structure is provided, the bioscaffold comprising a plurality of connected unit cells, each unit cell comprising a plurality of filaments composed of an extracellular material containing Collagen I and Collagen III, wherein each of the plurality of unit cells includes at least one opening connected to an internal volume.

The present invention provides: an extracellular matrix of which the biocompatibility is greatly increased through fat removal and decellularization and which contains growth factors so as to control physiological activities of cells; and a method for preparing the same. In addition, the extracellular matrix may be effectively obtained through a solvent extraction process using supercritical fluid, and a preservation solution comprising the extracellular matrix may be preserved for a long time by preventing contamination, and has increased manageability so that usability of the extracellular matrix may be greatly increased.

The present invention provides tissue scaffolds, methods of generating such scaffolds, and methods of use of such scaffolds to generate aligned and functional neural tissues for use in methods including regenerative medicine, wound repair and transplantation.

A method of preparing a decellularized placental membrane is provided. The method comprises removing cells from a pre-decellularized placental membrane comprising an amnion layer and a chorion layer to produce a decellularized placental membrane without separating the amnion layer from the chorion layer. The pre-decellularized placental membrane is obtained from an amniotic sac, and the decellularized placental membrane comprises the amnion layer and the chorion layer. Also provided is a decellularized placental membrane and a placenta-derived graft comprising the decellularized placental membrane. Further provided are the uses of the decellularized placental membrane or the placenta-derived graft.

Described are embodiments of a multilaminate or multiple layer implantable surgical graft with an illustrative graft comprising a remodelable collagenous sheet material, the graft including one or more interweaving members to stitch together the graft to help prevent the layers from delaminating or separating during handling and the initial stages of remodeling. The interweaving members may comprise lines of suture, thread, individual stitches, strips of material, etc. that are woven through the layers of biomaterial in a desired pattern. In one embodiment, the interweaving members comprise a pharmacologically active substance, such as a drug, growth factors, etc. to elicit a desired biological response in the host tissue. In another embodiment, the graft further comprises a reinforcing material, such as a synthetic mesh, within the layers of remodelable biomaterial and stitched together by one or more interweaving members.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

This disclosure provides a tissue-engineered transcatheter vein valve and methods of making such a tissue-engineered transcatheter vein valve. Methods of making the valve include casting or molding a polymer into a tubular structure having a first end and a second end, where the first end of the tubular structure is cast or molded around a tubular support structure and where the second end of the tubular structure is cast or molded in the absence of the support structure; everting the polymer at the second end through the support structure; anchoring the second end of the tubular structure to the support structure at a first position and a second position, where the anchored first position and the anchored second position result in commissures, forming leaflets therebetween.

The present disclosure provides, in various aspects, a method of forming a prefabricated innervated pre-vascularized pre-laminated (PIPP) flap having a stoma or lumen. The method includes providing a cell construct including skin cells and/or mucosa cells. The method further includes forming an integrated in vivo composite at a donor site by grafting the cell construct onto a muscle. The method further includes stabilizing the composite on an obturator component. The method further includes developing a microvascular system in the composite by retaining it in vivo at the donor site for a predetermined period of time. The method further includes removing the obturator component from the stoma or lumen. In certain aspects, the present disclosure also provides a method of restoring a defect including damaged or surgically removed soft tissue using a PIPP flap. In certain aspect, the present disclosure also provides an obturator component for maintaining the stoma or lumen.

The present invention relates to a tissue fusion composition having tissue adhesion and differentiation characteristics, and a preparation method therefor. In the present invention, a tissue fusion composition in the form of a gel or sheet is prepared using stem cells and stem cell-derived extracellular matrix, and it has been confirmed that the composition adheres and binds superbly to biological tissues and can be differentiated into cartilage, bone, cornea, growth plate, and the like, and thus the composition can be used as an adhesive and a differentiation agent in regenerative therapy of damaged tissues or organs, therefore being ultimately and effectively usable as an agent for tissue fusion.

An implantable and biodegradable polymeric matrix with reduced foreign body response for the regeneration and/or reconstruction and/or creation of soft and connective tissue and/or organs is provided. The matrix has a density equal to or lower than 40 kg/m.sup.3, a plurality of local thicknesses of a solid component with an arithmetic mean equal to or lower than 95 μm, an average size of pores/void spaces equal to or lower than 15,000 μm, a surface roughness R.sub.a of the solid component with an arithmetic mean equal to or lower than 3 μm; and a contact angle θ of the solid component lower than 110°, preferably in the range 10° to 90°, and more preferably in the range 30° to 60°.