Sheet structures for regenerating damaged or diseased mammalian tissue that are formed from acellular dermal mammalian tissue. The acellular dermal mammalian tissue includes extracellular matrix (ECM) and a supplemental bioactive component. The supplemental bioactive component can comprise a nucleic acid, such as RNA, and/or a cell, such as an embryonic stem cell. The sheet structures induce angiogenesis and, thereby, regeneration of new mammalian tissue.

Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.

Cardiovascular prostheses for treating, reconstructing and replacing damaged or diseased cardiovascular tissue. The prostheses are in the form of sheet structures that are formed from a composition that includes adolescent mammalian dermal tissue and a plurality of exogenously added exosomes. In some instances, the composition also includes at least one exogenously added cell, such as an embryonic stem cell, a mesenchymal stem cell and a hematopoietic stem cell. The prostheses are adapted to induce neovascularization, stem cell proliferation and, thereby, remodeling of damaged biological tissue and regeneration of new biological tissue and structures, when the prostheses are delivered to the damaged biological tissue.

The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.

Provided are tissue repair and sealing devices, and methods for the use of tissue repair and sealing devices, for use in both minimally invasive surgical (MIS) procedures and open, non-MIS procedures to rapidly repair tissue fenestrations and create a pressure-resistant, watertight seal in a tissue barrier. Tissue repair and sealing devices disclosed herein comprise an integrated graft and deployable clasp assembly and an applicator assembly having a clasp retain and release member that is slidably connected to a folded, deployable clasp. The applicator assembly places a graft on a tissue inner surface and a deployable clasp on a tissue outer surface to secure the graft to the tissue inner surface to, thereby, repair a tissue fenestration and create a watertight barrier.

The invention relates to the complex of synthetic polymer and natural extracellular matrix for vascular grafts and their preparation methods. The components of biodegradable synthetic polymer in the preparation process can be chosen with different material proportions. The scaffolds with different fiber diameters, different fiber arrangements, different pore sizes and different pore structures can be prepared by electro-spinning, wet-spinning, melt-spinning, 3D printing, pouring, phase separation, particle leaching and other technologies. Among them, the natural extracellular matrix components come from a wide range of sources such as vascular tissues from different kinds of animals including arteries and veins of pigs and cattle or vascular tissues from human donors including umbilical cord vessels, etc. And its composition and content can be flexibly adjusted according to the demand. The composites and artificial vessels prepared by this technology not only have good mechanical properties, controllable spatial structure and suitable degradation rate, but also have excellent biocompatibility and bioactivity. The preparation process of the invention is simple, the controllability is high, the preparation condition is mild, and is suitable for large-scale industrial production.

The present invention is a multi-stage treatment that heals tissue or organ damage (e.g., linear defects, fissures, and fibrillations, as well as focal and large defects) in collagen-rich tissues and organs such as articular cartilage. The present invention includes methods 1) to prime tissues in preparation for treatment, which comprises “melting” the tissue matrix, 2) to add or fill the damaged area with a “melding” agent, comprising of endogenous or exogenous tissue matrix, with or without cells, with or without exogenous biomaterials, and with or without endogenous or exogenous enzymes, such that the melding agent enhances anchoring into the defect for the purpose of integration and/or tissue healing. The Melt-and-Meld process can also be applied in conjunction with any existing treatments of tissue or organ defects.

The invention is a biodegradable polymer composition comprising a particulate biodegradable polymer, particulate extracellular matrix (ECM) derived from a mammalian tissue source, a therapeutic component that abates growth of bacteria and a liquid medium. The biodegradable polymer can change quality upon contact with a physiological parameter, such as temperature or pH that causes, for example, a liquid polymer to gel or harden. The polymer composition is adapted to induce regeneration of tissue in vivo.

The present invention relates to a method of promoting skin rejuvenation in a subject. The method comprises administering to the subject an effective amount of a cellular extract of epithelial or mesenchymal stem/progenitor cells isolated from the amniotic membrane of the umbilical cord, wherein the cellular extract contains growth factors and peptides and is in the form of a supernatant into which the epithelial or mesenchymal stem/progenitor cells secrete the growth factors and peptides.

The present disclosure provides methods and systems for printing a three-dimensional (3D) material. In some examples, a method for printing a 3D biological material comprises providing a media chamber comprising a medium comprising (i) a plurality of cells and (ii) one or more polymer precursors. Next, at least one energy beam may be directed to the medium in the media chamber along at least one energy beam path that is patterned into a 3D projection wherein the x, y, and z dimensions may be simultaneously accessed in accordance with computer instructions for printing the 3D biological material in computer memory, to form at least a portion of the 3D biological material comprising (i) at least a subset of the plurality of cells, and (ii) a polymer formed from the one or more polymer precursors.