A tissue graft product comprising two or more layers of material wherein each layer comprises extracellular matrix (ECM) or polymeric material and wherein the layers are laminated together by interlocking portions of one layer with portions of another layer.

A mesh implant is disclosed and comprises a single sheet, highly porous, adhesion-resistant, tensile surgical implant composed of a gradually biodegradable synthetic polymer material that is electrospun into nanofibers and randomly stacked to form a three-dimensional (3D) mesh. The mesh implant is for tissue repair and hernia repair. The single-sheet design reduces the foreign material that make up the mesh implant, which minimizes mesh implant rejection. The gradually biodegradable nature of the mesh implant guarantees that the mesh stays in place and supports the repaired site long enough until a proper scar tissue has built up, after which the mesh implant disappears from the body, therefore preventing pain and irritability. The 3D design of the nanofibrous network and the high porosity of the mesh implant facilitate cell attachment, infiltration, and proliferation, all necessary for scar tissue formation, mesh integration, wound healing, and proper defect closure.

Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.

The present invention relates in part to hybrid hydrogel scaffolds including a decellularized extracellular matrix (dECM) tissue, and a synthetic polymer. The dECM may include any suitable tissue including for example, lung tissue, heart tissue, heart-lung block tissue, skin tissue, liver tissue, pancreatic tissue, kidney tissue, and the like.

The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

A tissue graft product comprising two or more layers of material wherein each layer comprises extracellular matrix (ECM) or polymeric material and wherein the layers are laminated together by interlocking portions of one layer with portions of another layer.

The present disclosure provides tissue products produced from extracellular tissue matrices. The tissue products can include acellular extracellular matrices including combinations of different tissue types. The combination can harness various properties of the different tissues to provide improved composite structures with desired mechanical and/or biologic properties.

The present disclosure provides methods for producing bioengineered tissue along with an apparatus and other relevant compositions employed in generation thereof.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

A skin-substitute is constructed by homogenizing an acellular dermal tissue matrix into a slurry, pouring the slurry into a mold, and lyophilizing the slurry.