A bioactive filamentary structure includes a sheath coated with a mixture of synthetic bone graft particles and a polymer solution forming a scaffold structure. In forming such a structure, synthetic bone graft particles and a polymer solution are applied around a filamentary structure. A polymer is precipitated from the polymer solution such that the synthetic bone graft particles and the polymer coat the filamentary structure and the polymer is adhered to the synthetic bone graft particles to retain the graft particles.

The invention provides a method for delivering cells across the surface of a tissue, the method comprising distributing the cells on and/or within a biomaterial to form a cell bandage and applying the cell bandage to the surface, wherein, after application of the cell bandage to the surface of the tissue, the cells are released from the cell bandage. Further provided is a method for bonding two or more tissues, the method comprising providing a cell bandage in intimate contact with the surfaces to be joined, wherein the cell bandage comprises a biomaterial, said biomaterial having cells distributed on and/or within it.

The present invention discloses adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF), their compositions and kits containing the ADSC's and their enriched fractions, and enzyme blends useful for their isolation, as well as methods of their isolation and use.

A method of making a prosthetic heart valve may include providing an annular stent having a plurality of annularly spaced commissure portions having tips, covering each of the tips with a first fabric cover, covering the first fabric covers and the remainder of the stent with a second fabric cover, covering the second fabric cover with a first tissue membrane, and covering the outside of the first tissue membrane with a second tissue membrane, the second tissue membrane forming leaflet portions that extend inwardly between the commissure portions.

The invention relates to a double-microenvironment three-layer bionic hydrogel scaffold and a preparation method and application thereof, which are characterized in that includes a cartilage layer GL-HPKGN, an intermediate layer GL-GMA, and a bone layer GL-HP/GMAAT; by an enzymatic crosslinking reaction based on hydroxyphenylpropionic acid (HPA), kartogenin (KGN) is grafted onto gelatin to form a cartilage-specific microenvironment in GL-HPKGN; Based on hydroxyphenylpropionic acid (HPA), a dual crosslinking network was formed by enzyme crosslinking reaction and methacryloyl-glycidyl dimethicone (GMA) photo-crosslinking reaction, which was used to graft atorvastatin (AT) onto gelatin, forming GL-HP/GMAAT with bone-specific microenvironment; the intermediate layer GL-GMA was beneficial for forming a clear and defined cartilage-bone integrated structure. The introduction of the tide-line intermediate layer GL-GMA facilitated the formation of well-defined chondro-bone integrated structures.

A composition including delipidated, decellularized adipose tissue and delipidated, decellularized fascial tissue is provided. The composition may further include exogenous tissuegenic cells, an exogenous growth-inductive substance, and/or a carrier. The composition is suitable for implantation into a living body in plastic surgery procedures, including reconstructive or cosmetic surgery procedures, wound care procedures or other procedures of regenerative medicine. A method of preparing an acellular soft tissue-derived matrix from adipose tissue and fascial tissue is also provided. The method involves preparing a delipidated, decellularized adipose-derived matrix by delipidizing the adipose tissue and decellularizing the adipose tissue; preparing a delipidated, decellularized fascia-derived matrix by delipidizing the fascial tissue and decellularizing the fascial tissue; and combining the delipidated, decellularized adipose-derived matrix and the delipidated, decellularized fascia-derived matrix to produce said acellular soft tissue-derived matrix. The resulting Acellular soft tissue-derived matrix may be partially dried, substantially dried, or not dried.

An interarticular ligament prosthesis is formed from a plurality of high strength high modulus polymeric fibers. The fibers are independent and free from intrinsic inter-fiber shear coupling found in braided or bonded fibers. The ligament prosthesis is installed with tubular, bone screw anchors. The fibers of the ligament prosthesis pass through the central hole of the anchors and are knotted at one end. The exit holes of the anchors include ceramic eyelets with polished edges. The edges are rounded to a defined radius for desired fatigue life of the prosthesis.

The invention provides a method for delivering cells across the surface of a tissue, the method comprising distributing the cells on and/or within a sheet of biomaterial to form a cell bandage and applying the cell bandage to the surface, wherein, after application of the cell bandage to the surface of the tissue, the cells are released from the cell bandage. Further provided is a method for bonding two or more tissues, the method comprising providing a cell bandage in intimate contact with the surfaces to be joined, wherein the cell bandage comprises a sheet of biomaterial, said biomaterial having cells distributed on and/or within it. Also provided is a cell bandage for use in the methods of the invention.

Implants for promoting bone growth and methods of using same, the implants including a perforated placental membrane sheet wrapped around an osteoconductive material composed of bone chips, bone granules, bone powders or combinations thereof, the osteoconductive material being configured for providing a scaffold upon which bone growth can occur. The placental membrane sheet acts to maintain the osteoconductive material in a cohesive, organized configuration within a site of a patient where bone growth is to be induced. The perforations in the placental membrane sheet create passageways in the exterior of the implant through which the osteoconductive material can communicate with adjacent bone surfaces which are to be fused.

The present invention belongs to the in-situ regeneration induction in the field of regenerative medicine, especially the reconstruction of joint-like structures in mammals.