The present application relates to use of transglutaminases to treat various tissue matrix products. The methods can include application of a transglutaminase to a partially denatured collagen-containing tissue matrix and implantation of the tissue matrix. The transglutaminase can facilitate adhesion with another collagen-containing tissue matrix, tissue surrounding the tissue matrix after implantation, or both.

Tissue compositions and methods of preparation thereof are provided. The tissue compositions can be used to treat or regenerate muscle tissue. The compositions can be configured to provide increased strength compared to other muscle matrices.

A fiber assembly includes multiple fibers and nucleic acid fragments that crosslink the multiple fibers, and the fibers are microtubules or actin fibers.

Prosthetic heart valves having a conical shaped base valve structure formed from collagenous mammalian tissue. The base valve structure includes a plurality of elongated ribbon members that are positioned proximate each other in a joined relationship, wherein the elongated ribbon members are positioned adjacent each other and form a plurality of fluid flow modulating regions that open when fluid into and through the base valve structure exhibits a positive pressure relative to the exterior pressure, i.e., a positive pressure differential, wherein the fluid is allowed to be transmitted out of the base valve structure, and transition to a closed configuration when the pressure differential between the interior valve pressure and exterior pressure reduces, wherein the fluid is restricted from flowing out of the base valve structure.

A prosthetic valve comprising a conical shaped sheet structure and a support structure, the sheet structure having a closed distal end and a plurality of elongated ribbon members that are positioned proximate each other in a joined relationship, whereby the ribbon members form a plurality of fluid flow modulating regions that close when fluid flow through the valve exhibits a negative flow pressure and open when fluid flow through the valve exhibits a positive flow pressure, the support structure having at least one elongated cardiovascular structure engagement member that is associated with one of the ribbon members and adapted to engage a cardiovascular structure.

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.

A prosthetic valve comprising a tubular shaped sheet member comprising an extracellular matrix (ECM) composition, the sheet member having a plurality of ribbons having proximal and distal ends, the distal ends of the ribbons projecting from the sheet member proximal end.

The present disclosure describes methods of treating an injury in a subject using placental tissue streamers, engineered tissue placental tissue hybrids, suture placental tissue hybrids, placental tissue patch hybrids, and tissue hybrids, and the use of these compositions to repair, treat, or support an injury or degenerative process in a subject.

The present invention relates to preparation and use of nanocellulose fibrils or crystals such as disintegrated bacterial nanocellulose, tunicate-derived nanocellulose, or plant-derived nanocellulose, together with carbon nanotubes, as a biocompatible and conductive ink for 3D printing of electrically conductive patterns. Biocompatible conductive bioinks described in this invention were printed in the form of connected lines onto wet or dried nanocellulose films, bacterial cellulose membrane, or tunicate decellularized tissue. The devices were biocompatible and showed excellent mechanical properties and good electrical conductivity through printed lines (3.8.Math.10.sup.−1 S cm.sup.−1). Such scaffolds were used to culture neural cells. Neural cells attached selectively on the printed pattern and formed connective networks. The devices prepared by this invention are suited as bioassays to screen drugs against neurodegenerative diseases such as Alzheimer's and Parkinson's, study brain function, and/or be used to link the human brain with electronic and/or communication devices. They can also be implanted to replace neural tissue or stimulate guiding of neural cells. They can also be used to stimulate the heart by using electrical signaling or to repair myocardial infarction and/or damage related thereto.

Disclosed are electrospun scaffolds and methods of making electrospun fibers and electrospun fiber scaffolds. More particularly, the present disclosure relates to electrospun fibrous scaffolds of decellularized muscle tissue and methods of making fibers and fiber scaffolds by electrospinning.