Some embodiments of the disclosure provide a decellularized heart valve (DHV) composite, a preparation method of the DHV composite, and a use of the DHV composite. In some examples, the preparation method of the DHV composite includes the following steps: S1, conducting a reaction I on a DHV with a copper chloride-dopamine hydrochloride mixed solution to obtain a copper ion-modified DHV; and S2, conducting a reaction II on the copper ion-modified DHV with a GDF11 solution to obtain the DHV composite. In other examples, the present disclosure provides a use of the DHV composite in preparation of a tissue-engineered heart valve (TEHV). In further examples, the TEHV is a heart valve (HV) with remodeling and regeneration capabilities.

A method of preparing biological tissue for use as a component of an implant, in particular as part of a heart valve prosthesis, which can be implanted by catheter. The biological tissue is subjected to a dimensional and structural stabilization step and is dried. For the dimensional and structural stabilization, a combination of a first, polyethylene glycol-containing solution and at least one second, glycerol-containing solution is used.

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.

The present disclosure describes methods of treating an injury in a subject using placental tissue streamers, engineered tissue placental tissue hybrids, suture placental tissue hybrids, placental tissue patch hybrids, and tissue hybrids, and the use of these compositions to repair, treat, or support an injury or degenerative process in a subject.

Disclosed herein is a continuous tubular shaped valve member formed from crosslinked, acellular pericardium tissue. The tubular shaped valve member can have a lumen, an outer wall, a longitudinal axis, an inlet portion, and an outlet portion, the outlet portion comprising a top edge, wherein said top edge is disposed proximate said inlet portion, the outlet portion defining an outlet in fluid communication with said lumen of said tubular shaped valve member, said inlet portion defining an inlet in fluid communication with said lumen of said tubular shaped valve member.

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.

The present disclosure describes methods of treating an injury in a subject using placental tissue streamers, engineered tissue placental tissue hybrids, suture placental tissue hybrids, placental tissue patch hybrids, and tissue hybrids, and the use of these compositions to repair, treat, or support an injury or degenerative process in a subject.

Provided in this disclosure are various composite grafts having a trabecular scaffold with voids defined in at least a portion of the scaffold and a biological component positioned in at least some of the voids of the scaffold. The grafts may have a synthetic scaffold or a bone substrate scaffold. The grafts may be osteogenic, chondrogenic, osteochondrogenic, or vulnerary in nature. Also provided are methods of using the composite grafts to treat a tissue defect in a subject. Methods of manufacturing are also provided. Synthetic scaffolds are manufactured by additive manufacturing. Agitation is used to combine the biological component with the scaffold of the graft.

The present disclosure describes methods of treating an injury in a subject using placental tissue streamers, engineered tissue placental tissue hybrids, suture placental tissue hybrids, placental tissue patch hybrids, and tissue hybrids, and the use of these compositions to repair, treat, or support an injury or degenerative process in a subject.

A prosthetic valve comprising a tubular shaped sheet member comprising an extracellular matrix (ECM) composition, the sheet member having a plurality of ribbons having proximal and distal ends, the distal ends of the ribbons projecting from the sheet member proximal end.