Methods of making decellularized tendon matrix (DTM) and DTM hydrogels are provided. These compositions and hydrogels are useful for repairing tendon injuries and in some cases may be used by injection, arthroscopic procedures, or as adjuncts to traditional surgical repair.

The present invention provides a valve material having synergistic anti-coagulation and anti-calcification functions and a preparation method therefor. The preparation method comprises the following steps: performing glutaraldehyde cross-linking treatment on an animal-derived biological valve material; immersing the treated valve material in a blocking solution containing an amine compound for 0.5-6 h, thereby blocking the remaining aldehyde groups after glutaraldehyde cross-linking; then placing the valve material into a reaction solution containing an anticoagulant and a cross-linking agent, and performing cross-linking treatment for 6-24 h at 4° C.-37° C.; and finally washing and obtaining the valve material, and storing the valve material in a mixed solvent of glutaraldehyde or isopropyl alcohol/glycerol. The method can effectively solve the problem of calcification and thrombosis caused by residual aldehyde groups in a valve material prepared by the existing method. The valve material prepared by the present method can be used as a valve material required for aortic valve, pulmonary valve, venous valve, mitral valve and tricuspid valve replacement.

A method for producing xenogeneic bone graft, which is a material for use in bone tissue therapy, is provided. The method includes the steps of isolating the cancellous bones from cartilage and cortical bone by fragmenting them into pieces, washing the bone fragments with purified water to partially remove the organic phases and boiling them with purified water, contacting the bone fragments with a solvent for further removal/isolation of the organic phase, washing the bone fragments to remove the solvents used and other possible residues, subjecting them to a hydrothermal and/or solvothermal treatment with a solvent at a pressure of more than 1 atm and a temperature in the range of 100° C. to 300° C., after which washing them with purified water, and drying the cancellous bone fragments, for example, at a temperature in the range of 50° C. to 100° C. to dehydrate them.

A method for preparing a biological material for implanting provides irradiating at least a portion of the surface of the material with an accelerated Neutral Beam.

A method is provided for implanting a valve having at least one valve leaflet within the cardiovascular system of a subject. One step of the method includes preparing a substantially dehydrated bioprosthetic valve and then providing an expandable support member having oppositely disposed first and second ends and a main body portion extending between the ends. Next, the substantially dehydrated bioprosthetic valve is attached to the expandable support member so that the substantially dehydrated bioprosthetic valve is operably secured within the main body portion of the expandable support member. The expandable support member is then crimped into a compressed configuration and placed at a desired location within the cardiovascular system of the subject. Either before or after placement at the desired location, fluid or blood re-hydrates the substantially dehydrated bioprosthetic valve.

The present disclosure provides tissue products produced from adipose tissues, as well as methods for producing such tissue products. The tissue products can include acellular tissue matrices for treatment of a breast.

The present disclosure relates to tissue engineering, and more particularly to a method for treating or repairing rotator cuff or other tendon tears or damage using scaffold-free 3-dimensional engineered tendon constructs.

A method and system for making a bone plug using cortical bone material. A patient jaw having insufficient bone at a surgical site may be scanned to provide a 3D image which may be used to design a virtual bone plug and to fabricate the bone plug for placement within the patient. The bone plug may be formed from cortical bone that can be reconstituted and demineralized or demineralized and milled to shape.

The present invention discloses a bone void filler and a method for manufacturing the same by natural calcium-containing waste, which comprises steps of mixing 5-20 wt % of a calcium-containing waste powder, 5-20 wt % of acetic acid and a remaining weight percentage of water uniformly to obtain a mixing solution; adding 5-20 vol % of a diammonium hydrogen phosphate solution to the mixing solution to obtain a suspension; controlling a pH value of the suspension to obtain an alkaline solution; leaving the alkaline solution at room temperature for precipitation for 0.1 to 72 hours, centrifuging or suction filtrating the alkaline solution to obtain a precipitate, drying and grinding the precipitate to obtain hydroxyapatite; and mixing 30-60 wt % of a pore former and 30-60 wt % of the hydroxyapatite and a remaining weight percentage of a binder uniformly to form a mixture, compression molding the mixture in a mold and sintering the compression-molded mixture.

A method of lamellar corneal graft implantation is disclosed herein. In one or more embodiments, the method includes the steps of: (i) modifying a genetic component of a lamellar cornea or other tissue of an animal so that the lamellar cornea or other tissue of the animal can be used for human transplantation; (ii) decellularizing the lamellar cornea or other tissue ex vivo using chemical means; (iii) modifying a shape of the lamellar cornea or other tissue before or after transplantation; and (iv) applying a photosensitizer and ultraviolet radiation to the lamellar cornea or other tissue so as to crosslink collagen and intercellular proteins of the lamellar cornea or other tissue, kill the cells exposed to the photosensitizer, and eliminate an immune response by a host to the implanted lamellar cornea or the tissue.