A method for cartilage tissue engineering including fabricating a nanocomposite, injecting the nanocomposite into a defect site of cartilage, and forming a hydrogel in the defect site of the cartilage using a sol-gel transition responsive to increasing temperature of the nanocomposite from room temperature to 37° C. Fabricating a nanocomposite includes forming an activated copolymer by functionalizing a copolymer, forming a conjugated copolymer by grafting the activated copolymer to a polysaccharide, forming a protein-conjugated copolymer by crosslinking a protein with the conjugated copolymer, forming the nanocomposite by adding a plurality of nanoparticles to the protein-conjugated copolymer.

A biocompatible composite material for controlled release is disclosed, comprising a biocompatible metal oxide structure with a loaded network of pores. The pore network of the biocompatible composite material is filled with a uniformly distributed biologically active micellizing amphiphilic molecule, the size of these pores ranging from about 0.5 to about 100 nanometers. The material is characterized in that when exposed to phosphate-buffered saline (PBS), the controlled release of the active amphiphilic molecule is predominantly diffusion-driven over time.

Particular aspects of the present disclosure provide bio-resorbable and biocompatible compositions for bioengineering, restoring, or regenerating tissue or bone. In one embodiment, a biocompatible composition includes a three-dimensional porous or non-porous scaffold material comprising a calcium phosphate-based ceramic having at least one dopant therein selected from metal ion dopants or metal oxide dopants. The composition is sufficiently biocompatible to provide for a cell or tissue scaffold, and resorbable at a controlled resorption rate for controlled strength loss under body, body fluid or simulated body fluid conditions.

The invention pertains to the use of mesenchymal stromal cells (MSC) in the treatment of bone disorders or injuries. The invention provides MSC and preparations of specifically pooled MSC for use in the manufacturing of bone graft material for implanting into or attaching to bones in order to enhance bone regeneration after surgery or injury, or to treat various bone disorders, such as osteonecrosis. The invention provides bone graft material, a method for its production, bone graft implants, and medical methods and uses of the inventive products.

According to the invention, a preparation is described which contains at least one calcium compound selected from the group consisting of calcium phosphates, calcium fluorides and calcium fluorophosphates and hydroxyl derivatives and carbonate derivatives of these calcium salts, calcium hydroxides and calcium oxides precipitated using at least one protein component selected from proteins and protein hydrolysates, and at least one crosslinking agent for the protein component and/or non-set cement.

Compositions and methods for augmenting bone formation by administering isolated human mesenchymal stem cells (hMSCs) within a matrix provided. By adding calcium and/or phosphate ions to the matrix, one may foster greater bone regeneration.

A bioactive scaffold is provided. The bioactive scaffold includes an interconnected web of struts composed of a glass-ceramic material, the web of struts being printed as a three-dimensional structure from a filament composition having a bimodal distribution of glass-ceramic microparticles, wherein the bioactive scaffold has a porosity defined by spaces between struts of greater than or equal to about 40% to less than or equal to about 80% and an average pore size of greater than or equal to about 200 μm to less than or equal to about 400 μm. Methods of making the bioactive scaffold and treating bone defects using the bioactive scaffolds are also provided.

A medical instrument in which an inorganic salt solid such as apatite into which a peptide hormone or the like is embedded is placed so that a metal or the like is coated therewith, in which the inorganic salt solid is provided by controlled delay co-precipitation or the like in an unstable supersaturated calcium phosphate solution, and the medical instrument is exposed to ionizing radiation at a dose sufficient for sterilization.

The present invention deals with a bone implant matrix comprising a base matrix selected from the group comprising: —acellularized or acellularized non-demineralised bone matrix of any source, —matrix of natural mineral sources, —synthetic bioceramics matrix, or combinations of the above, wherein the surface of said base matrix is coated with an statistically homo-geneous composition which is a reinforcing mixture containing at least a bio-degradable polyester or co-polymer thereof, at least a gelatine or hydrolysed gelatine and at least an artificial Proline-Rich Peptide.

A biphasic calcium phosphate/hydroxyapatite (CAP/HAP) bone substitute material having a sintered CAP core and a closed epitactically grown layer of nanocrystalline HAP deposited on the external surface of the sintered CAP core, wherein the closed epitactically grown layer of nanocrystalline HAP deposited on the external surface of the sintered CAP core has a homogeneous coarse external surface comprising flat crystal platelets, which shows an enhanced osteogenic response, a method of promoting bone formation, bone regeneration and/or bone repair by implanting the biphasic calcium phosphate/hydroxyapatite (CAP/HAP) bone substitute material, and a process of preparation thereof.