The present invention relates to a method of providing a graft scaffold for cartilage repair, particularly in a human patient. The method of the invention comprising the steps of providing particles and/or fibres; providing an aqueous solution of a gelling polysaccharide; providing mammalian cells; mixing said particles and/or fibres, said aqueous solution of a gelling polysaccharide and said mammalian cells to obtain a printing mix; and depositing said printing mix in a three-dimensional form. The invention further relates to graft scaffolds and grafts obtained by the method of the invention.

The present invention relates to a method of providing a graft scaffold for cartilage repair, particularly in a human patient. The method of the invention comprising the steps of providing particles and/or fibres; providing an aqueous solution of a gelling polysaccharide; providing mammalian cells; mixing said particles and/or fibres, said aqueous solution of a gelling polysaccharide and said mammalian cells to obtain a printing mix; and depositing said printing mix in a three-dimensional form. The invention further relates to graft scaffolds and grafts obtained by the method of the invention.

The present invention relates to a method of providing a graft scaffold for cartilage repair, particularly in a human patient. The method of the invention comprising the steps of providing particles and/or fibres; providing an aqueous solution of a gelling polysaccharide; providing mammalian cells; mixing said particles and/or fibres, said aqueous solution of a gelling polysaccharide and said mammalian cells to obtain a printing mix; and depositing said printing mix in a three-dimensional form. The invention further relates to graft scaffolds and grafts obtained by the method of the invention.

A hydrogel composite is provided. The hydrogel composite comprises a modified poloxamer having a first charge moiety and a peptide having a second charge moiety, wherein the first charge moiety and the second charge moiety are oppositely charged for ionic interaction between the modified poloxamer and the peptide, and wherein at least one of the modified poloxamer and the peptide comprises a crosslinkable moiety. In particular, the modified poloxamer is a pluronic monocarboxylate activated by dimethylaminopyridine and triethanolamine; the peptide is a gelatin methacrylate. A bioshaping method using the hydrogel composite is provided, as well as a three-dimensional network obtained by the bioshaping method.

A hydrogel composite is provided. The hydrogel composite comprises a modified poloxamer having a first charge moiety and a peptide having a second charge moiety, wherein the first charge moiety and the second charge moiety are oppositely charged for ionic interaction between the modified poloxamer and the peptide, and wherein at least one of the modified poloxamer and the peptide comprises a crosslinkable moiety. In particular, the modified poloxamer is a pluronic monocarboxylate activated by dimethylaminopyridine and triethanolamine; the peptide is a gelatin methacrylate. A bioshaping method using the hydrogel composite is provided, as well as a three-dimensional network obtained by the bioshaping method.

A hydrogel composite is provided. The hydrogel composite comprises a modified poloxamer having a first charge moiety and a peptide having a second charge moiety, wherein the first charge moiety and the second charge moiety are oppositely charged for ionic interaction between the modified poloxamer and the peptide, and wherein at least one of the modified poloxamer and the peptide comprises a crosslinkable moiety. In particular, the modified poloxamer is a pluronic monocarboxylate activated by dimethylaminopyridine and triethanolamine; the peptide is a gelatin methacrylate. A bioshaping method using the hydrogel composite is provided, as well as a three-dimensional network obtained by the bioshaping method.

The invention features a composition comprising a self-healing interpenetrating network hydrogel comprising a first network and a second network. The first network comprises covalent crosslinks and the second network comprises ionic or physical crosslinks. For example, the first network comprises a polyacrylamide polymer and second network comprises an alginate polymer.

The invention features a composition comprising a self-healing interpenetrating network hydrogel comprising a first network and a second network. The first network comprises covalent crosslinks and the second network comprises ionic or physical crosslinks. For example, the first network comprises a polyacrylamide polymer and second network comprises an alginate polymer.

The invention features a composition comprising a self-healing interpenetrating network hydrogel comprising a first network and a second network. The first network comprises covalent crosslinks and the second network comprises ionic or physical crosslinks. For example, the first network comprises a polyacrylamide polymer and second network comprises an alginate polymer.

Bioactive porous bone graft implants in various forms suitable for bone tissue regeneration and/or repair, as well as methods of use, are provided. The implants are formed of bioactive glass and have an engineered porosity. The implants may take the form of a putty, foam, fibrous cluster, fibrous matrix, granular matrix, or combinations thereof and allow for enhanced clinical results as well as ease of handling.