The present application provides an injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells, and a preparation method and application thereof. The present application includes: preparing hydroxypropyl chitin from chitin through modification, preparing a composite with collagen and sodium hyaluronic, constructing the injectable temperature-sensitive composite hydrogel, loading adipose-derived mesenchymal stem cells of New Zealand rabbit and Genipin, and finally forming in-situ the injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells at the physiological temperature. The hydrogel prepared in the present application is of a three-dimensional porous structure, which is conducive to transferring of nutrients and metabolic waste, so as to provide an excellent microenvironment for the growth of cells, helping maintain survival rate and biological activity of the adipose-derived mesenchymal stem cells, and promoting differentiation of the adipose-derived mesenchymal stem cells into cartilage tissue, while having high mechanical strength, and thus can be widely used in cartilage tissue engineering.

The present application provides an injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells, and a preparation method and application thereof. The present application includes: preparing hydroxypropyl chitin from chitin through modification, preparing a composite with collagen and sodium hyaluronic, constructing the injectable temperature-sensitive composite hydrogel, loading adipose-derived mesenchymal stem cells of New Zealand rabbit and Genipin, and finally forming in-situ the injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells at the physiological temperature. The hydrogel prepared in the present application is of a three-dimensional porous structure, which is conducive to transferring of nutrients and metabolic waste, so as to provide an excellent microenvironment for the growth of cells, helping maintain survival rate and biological activity of the adipose-derived mesenchymal stem cells, and promoting differentiation of the adipose-derived mesenchymal stem cells into cartilage tissue, while having high mechanical strength, and thus can be widely used in cartilage tissue engineering.

The present application provides an injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells, and a preparation method and application thereof. The present application includes: preparing hydroxypropyl chitin from chitin through modification, preparing a composite with collagen and sodium hyaluronic, constructing the injectable temperature-sensitive composite hydrogel, loading adipose-derived mesenchymal stem cells of New Zealand rabbit and Genipin, and finally forming in-situ the injectable temperature-sensitive composite hydrogel containing adipose-derived mesenchymal stem cells at the physiological temperature. The hydrogel prepared in the present application is of a three-dimensional porous structure, which is conducive to transferring of nutrients and metabolic waste, so as to provide an excellent microenvironment for the growth of cells, helping maintain survival rate and biological activity of the adipose-derived mesenchymal stem cells, and promoting differentiation of the adipose-derived mesenchymal stem cells into cartilage tissue, while having high mechanical strength, and thus can be widely used in cartilage tissue engineering.

Disclosed herein are embodiments of orthopedic devices. In several embodiments, orthopedic device comprises a biocompatible covering. In several embodiments the biocompatible covering is a collagen-based material. In several embodiments, the collagen-based material is crosslinked using an epoxide-based crosslinking agent (e.g., a diepoxide, triepoxide, etc.). In several embodiments, after a precursor crosslinked collagen-based material is prepared (e.g., by subjecting it to crosslinking conditions), residual crosslinking agent in the precursor material is quenched. In several embodiments, it has been surprisingly found that, by subjecting the precursor crosslinked collagen based material to a quenching reaction (to provide the crosslinked collagen-based material), improved properties are obtained (e.g., lower toxicity lower cytotoxicity, etc.). In several embodiments, the crosslinked collagen-based material is fabricated into an orthopedic device or used to prepare an orthopedic device (e.g., implant).

Disclosed herein are embodiments of orthopedic devices. In several embodiments, orthopedic device comprises a biocompatible covering. In several embodiments the biocompatible covering is a collagen-based material. In several embodiments, the collagen-based material is crosslinked using an epoxide-based crosslinking agent (e.g., a diepoxide, triepoxide, etc.). In several embodiments, after a precursor crosslinked collagen-based material is prepared (e.g., by subjecting it to crosslinking conditions), residual crosslinking agent in the precursor material is quenched. In several embodiments, it has been surprisingly found that, by subjecting the precursor crosslinked collagen based material to a quenching reaction (to provide the crosslinked collagen-based material), improved properties are obtained (e.g., lower toxicity lower cytotoxicity, etc.). In several embodiments, the crosslinked collagen-based material is fabricated into an orthopedic device or used to prepare an orthopedic device (e.g., implant).

A shape memory polymer hydrogel that is biodegradable, includes antimicrobial agents, and has a tunable drug delivery is used for wound healing internally and externally. The shape memory polymer is synthesized using a combination of hydrophilic precursors that are configured to have two to four functional end groups, with at least one component that has at least three functional groups. The synthesis route provides for a covalently crosslinked thermoset hydrogel. The chemistry can be tuned to provide desired transition temperatures for delivery (e.g. below 37° C.) and desired pore sizes for healing (e.g. 250-500 μm).

A shape memory polymer hydrogel that is biodegradable, includes antimicrobial agents, and has a tunable drug delivery is used for wound healing internally and externally. The shape memory polymer is synthesized using a combination of hydrophilic precursors that are configured to have two to four functional end groups, with at least one component that has at least three functional groups. The synthesis route provides for a covalently crosslinked thermoset hydrogel. The chemistry can be tuned to provide desired transition temperatures for delivery (e.g. below 37° C.) and desired pore sizes for healing (e.g. 250-500 μm).

A shape memory polymer hydrogel that is biodegradable, includes antimicrobial agents, and has a tunable drug delivery is used for wound healing internally and externally. The shape memory polymer is synthesized using a combination of hydrophilic precursors that are configured to have two to four functional end groups, with at least one component that has at least three functional groups. The synthesis route provides for a covalently crosslinked thermoset hydrogel. The chemistry can be tuned to provide desired transition temperatures for delivery (e.g. below 37° C.) and desired pore sizes for healing (e.g. 250-500 μm).

Aspects of this disclosure relate to a combination of techniques and/or materials that can be used to form a synthetic scaffold for solid and/or hollow organs or tissue. In some embodiments, methods are provided that involve assembling a synthetic scaffold using a first material for a first structural component and a second material for a second structural component, in which the first or second structural component in a perfusion pathway. In some embodiments, materials (e.g. synthetic materials) for the scaffold are printed, molded, cast, polymerized or electrospun. In some embodiments, a scaffold may mimic a natural scaffold or several features of a natural scaffold.

Aspects of this disclosure relate to a combination of techniques and/or materials that can be used to form a synthetic scaffold for solid and/or hollow organs or tissue. In some embodiments, methods are provided that involve assembling a synthetic scaffold using a first material for a first structural component and a second material for a second structural component, in which the first or second structural component in a perfusion pathway. In some embodiments, materials (e.g. synthetic materials) for the scaffold are printed, molded, cast, polymerized or electrospun. In some embodiments, a scaffold may mimic a natural scaffold or several features of a natural scaffold.