In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.

Fluid access devices include a machine-side hydraulic circuit and a patient-side hydraulic circuit, and are configurable between a connected state and at least one disconnected state. In the connected state, fluid flows between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In the disconnected state, fluid does not flow between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In some disconnected states, fluid recirculates through at least one of the machine-side hydraulic circuit or the patient-side hydraulic circuit in the disconnected state.

A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.

An apparatus for monitoring cells, biological materials, and/or particles in a fluid flow system including a plurality of fluid sampling loops arranged substantially in parallel adapted to allow the fluid to enter each of the plurality of fluid sampling loops sequentially at different time intervals. Each of the plurality of fluid sampling loops includes at least one filtration element adapted to retain the cells, biological materials, and/or particles when the fluid passes through each of the plurality of fluid sampling loops.

Systems and methods are provided for separating blood into two or more components. A blood separation system includes a blood separation device and a fluid flow circuit configured to be mounted to the blood separation device. The blood separation device includes a centrifugal separator and a spinning membrane separator drive unit incorporated into a common case, which allows for fluid separation by two different methods. Depending on the separation procedure to be carried out, the fluid flow circuit paired with the blood separation device may include only one separation chamber configured to be mounted to the centrifugal separator or spinning membrane separator drive unit or two separation chambers, with one being mounted to the centrifugal separator and the other to the spinning membrane separator drive unit. The system may be used to separate and collect any combination of red blood cells, plasma, and platelets.

During a first stage of a priming procedure, a priming fluid is conveyed into a spinning membrane separator via a filtrate outlet port so as to convey air out of the spinning membrane separator via an inlet port and a retentate outlet port of the spinning membrane separator. During an optional second stage of the priming procedure, the priming fluid is conveyed into the spinning membrane separator via the inlet port so as to convey air out of the spinning membrane separator via the retentate outlet port. A rotor positioned within a housing of the spinning membrane separator may be rotated with respect to the housing during the first and second stages to force air from within the rotor into an annulus defined between the rotor and the housing for more complete priming of the spinning membrane separator.

The invention, provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage,

An apparatus (1) for providing agitation inside a patient's body cavity (C) is such that substantially all the fluid and/or content present in the body cavity (C) is agitated and comprises a gas source (6, 7); means for delivering gas (2) from the gas source (6, 7) into a patient's body cavity (C); and means for recovering gas (9) from the patient's body cavity (C). A gas-fluid separation device (9) and a securing device for use with the apparatus (1), a method for providing agitation inside a patient's body cavity (C), a method for administering a therapeutic fluid into a patient's cavity (C), a method for securing the agitation apparatus (1) to a patient using the securing device and a method for the separation of a gas from a fluid using the gas-fluid separation device (9) are also disclosed.

A monitoring apparatus is to be applied to an assisted circulation apparatus, the assisted circulation apparatus being connected to a living body, transferring blood removed from the living body to a membrane lung by a blood transfer pump, and gas-exchanging and oxygenating the blood in the membrane lung in parallel with a native lung, and the monitoring apparatus includes a calculation unit that calculates a blood gas-exchanging state index indicating a gas-exchanging state of blood by the assisted circulation apparatus.

Provided is a chamber configuration for a reverse flow centrifuge, and a reverse flow centrifuge system configured for low fluid volume and small radius rotation. The compact reverse flow centrifuge system has a reusable subsystem and a single use replaceable subsystem. The replaceable subsystem comprises a separation chamber, fluid delivery manifold and rotational mounting connecting the separation chamber to the fluid manifold. The single use replaceable subsystem provides a closed environment for execution of reverse flow centrifugation processes. The separation chamber has a substantially conical fluid enclosure portion connected to a neck portion, and a dip tube extends centrally through the conical fluid enclosure to provide a fluid path to the tip of the conical fluid enclosure.