A method for generating atmospheric pressure cold plasma inside a hand-held unit discharges cold plasma with simultaneously different rf wavelengths and their harmonics. The unit includes an rf tuning network that is powered by a low-voltage power supply connected to a series of high-voltage coils and capacitors. The rf energy signal is transferred to a primary containment chamber and dispersed through an electrode plate network of various sizes and thicknesses to create multiple frequencies. Helium gas is introduced into the first primary containment chamber, where electron separation is initiated. The energized gas flows into a secondary magnetic compression chamber, where a balanced frequency network grid with capacitance creates the final electron separation, which is inverted magnetically and exits through an orifice with a nozzle. The cold plasma thus generated has been shown to be capable of accelerating a healing process in flesh wounds on animal laboratory specimens.

A radio frequency system for a radiation therapy machine includes a first portion containing a first gas at a first pressure, a second portion containing a second gas at a second pressure, the second gas being different from the first gas, and a component between the first portion and the second portion, the component containing the first gas at the first pressure.

This disclosure relates to stabilized anti-cancer atmospheric plasma (CAP)-stimulated media, to methods for preparing such media, and to methods of treatment using such media.