A method to divide liposuction fat into aliquots for use and cryopreservation purposes, the method comprising: providing a taking container that contains adipose material removed by liposuction, the adipose material including fat and aqueous fluid; providing a plurality of cryopreservation containers; taking a quantity of the adipose material from the taking container, keeping the quantity of the adipose material isolated from an external environment; separating by gravity the fat from the aqueous fluid in the adipose material of the taken quantity; and transferring the separated fat into one or more cryopreservation containers, to define isolated aliquots of fat.

The invention relates to a process for decontaminating a biological material by partitioning and inactivation techniques. The process includes the formation of a three-layer liquid system (a-b-c), where the layers are placed one onto the other and are different from each other in density and/or miscibility properties. The upper layer (a) has an aqueous solution of the biological material to decontaminate; the lower layer (c) has the solution inactivating viruses, prions or bacteria; the intermediate layer (b), interposed between the upper and lower layers, separates and protects the biological material from the inactivating solution which has an organic solvent or a mixture of more such solvents, immiscible at least with the upper layer. Moreover, the invention provides specific indications about the density difference between the layers in order to preserve system stability and, at the same time, protect the efficiency of decontamination and the biological activity of the material undergoing decontamination.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio ? value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio ? value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

A system (1) to divide liposuction fat into aliquots, comprising taking means (2, 3) to take from a container a quantity of adipose material, made available by means of liposuction, and to preserve sealed the quantity taken, the system (1) also comprising at least a separation syringe (4) able to be put in communication with said taking means (2, 3), and able to contain at least a portion of said quantity, the separation syringe (4) being provided so as to separate, by gravity, the fat from the aqueous fluids composing the adipose material, first connection means (5) to put in sealed communication the taking means (2, 3) with the separation syringe (4), at least a rejection container (6), able to be put in communication with the separation syringe (4) so as to receive the aqueous fluids, following their ejection from the separation syringe (4), at least a syringe device (7, 8) able to be put in sealed communication with the separation syringe (4) so as to receive an aliquot of fat, following its ejection from the separation syringe (4), the syringe device (7, 8) being able to contain sealed and to inject fat to make infiltrations, and second connection means (9) able to put in sealed communication alternately the separation syringe (4) and the rejection container (6) or the separation syringe (4) and the syringe device (7, 8), where the syringe device (7, 8) comprises at least a receptacle (7) and at least an infiltration syringe (8), connected sealed to one another, and is connectable in a removable manner to the second connection means (9) to define, as a result of their filling with fat, a portable unit to contain and to inject an aliquot of fat.

Disclosed is a system for the collection, processing and reuse of amniotic fluid and placental aspirate at a C-section site. The system includes a canister positioned along the vacuum line through which the amniotic fluid and placental aspirate is suctioned. The canister has a coil whereby the heavier cellular components, including stem cells, platelets and growth factors, are separated coincident with the surgical procedure. The canister has a port whereby the heavier cellular material can be removed from the canister. The heavier cellular material can be then applied to the wound site of the cesarean section patient. The system disclosed allows for the processing of the amniotic fluid and placental aspirate to take place in the same room as the surgical procedure. A kit and method are also provided.

The present disclosure provides a system for using an in-line processing device with an apheresis device. The system includes a pressure system that includes a container configured to receive cells collected using the apheresis device and is configured to change a pressure of the container so as to direct the cells collected using the apheresis device to the in-line processing device.

A method is provided in a centrifugal blood processing system for adding replacement fluid without a dedicated peristaltic pump to blood components being returned to the donor. A disposable blood processing set for use in the method comprises a hermetically sealed set of blood bags, connecting tubes, needles or connectors, and supporting structures with a replacement fluid line coupled directly to a return reservoir without contact with an intervening pump.

A blood reinfusion system for processing autologous blood for reinfusion into a patient. The system includes a blood reinfusion system having a blood processing canister with an internal fluid passageway. The internal fluid passageway includes a venturi region having a gravity-driven filtering system that is adapted to extract impurities from autologous blood without damaging the erythrocytes in the blood and, hence, inducing hemolysis of the erythrocytes in the blood, and loss of seminal native blood components, including native erythrocytes, platelets, white blood cells, plasma proteins and antibodies.

Disclosed is a system for the collection, processing and reuse of amniotic fluid and placental aspirate at a C-section site. The system includes a canister positioned along the vacuum line through which the amniotic fluid and placental aspirate is suctioned. The canister has a coil whereby the heavier cellular components, including stem cells, platelets and growth factors, are separated coincident with the surgical procedure. The canister has a port whereby the heavier cellular material can be removed from the canister. The heavier cellular material can be then applied to the wound site of the cesarean section patient. The system disclosed allows for the processing of the amniotic fluid and placental aspirate to take place in the same room as the surgical procedure. A kit and method are also provided.