Embodiments are described that include systems and methods for separating components of a composite fluid, e.g., whole blood. Some embodiments provide for processing a composite fluid by subjecting a volume of the fluid to a first centripetal acceleration for an initial separation, followed by a second centripetal acceleration for a second separation.

An autologous cell concentrating system and method are disclosed. The system has a blood separation component, a first vessel, a second vessel, a first valve, a second valve, and a concentration and flow logic and control component. The concentration and flow logic and control component is configured to: determine a first volume of a target cell-poor fraction in the first vessel to mix with a target cell-rich fraction in the second vessel in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range; and control the second valve to transfer the first volume of the target cell-rich fraction from the first vessel to the second vessel to form the target cell-rich concentrate. The target concentration range is between 1.0 and 1.510.sup.6 target platelets/L.

A system and a method for collecting plasma has a separator, a donor line, an anticoagulant line, a touchscreen, and a controller. The controller controls operation of the system and receives donor parameters electronically from a donor management system. The controller uses a target volume for plasma product and/or raw plasma which is based at least in part on donor height and weight used to calculate total donor blood volume, the target volume for plasma product and/or raw plasma based on the total donor blood volume. The controller controls the system to operate at least three draw and return phases to withdraw whole blood from a donor and separate the whole blood into the plasma product and the red blood cells and to return the red blood cells to the donor

A method of collecting mononuclear cells, comprising separating whole blood into cellular components and platelets suspended in plasma, separating the platelets suspended in plasma into platelet concentrate and platelet-poor plasma, combining the cellular components with the platelet-poor plasma to form a first mixture, and separating the first mixture into mononuclear cells and at least one component.

Described are embodiments that include methods and devices for separating components from multi-component fluids. Embodiments may involve use of separation vessels and movement of components into and out of separation vessels through ports. Embodiments may involve the separation of plasma from whole blood. Also described are embodiments that include methods and devices for positioning portions, e.g., loops, of disposables in medical devices. Embodiments may involve use of surfaces for automatically guiding loops to position them into a predetermined position.

Provided is a blood component separation device that can shorten the overall time to collect high-concentration platelet liquid for blood component donation, thereby reducing time to keep a donor for blood drawing. The blood component separation device includes a temporary storage bag (also used as a buffy coat bag) which is also used as a whole blood bag for storing whole blood drawn from the donor. A control means performs whole blood drawing from the donor in parallel with performing at least either of a circulation flow step and an acceleration step, thereby storing the collected whole blood in the temporary storage bag.

A triple syringe system that allows for a larger output of autologous conditioned plasma (ACP) is provided. Autologous blood or bone marrow aspirate can be introduced into a nested set of syringes, which are subjected to centrifugation to obtain two or more of blood or bone marrow fractions including a first fraction and a second fraction. The first fraction can be transferred from the first syringe to the second and third syringes to obtain the ACP.

The present disclosure relates to methods and apparatus for producing platelet rich plasma, bone marrow mononuclear cells, stromal vascular fraction from adipose tissue, and other concentrated or enriched biological fluids.

Provided herein is a triple syringe system that allows for a larger combined output of PRP (platelet rich plasma) and PPP (platelet poor plasma). The multi-syringe system allows for the connection of two or more additional syringes. The fractions may be extracted with the multi-syringe system of the present invention at different sequential times, or at the same time.

Methods and systems for the automated collection of plasma from a donor are disclosed. The methods and systems deliver anticoagulant to whole blood and/or to selected and separated components at selected times to provide a sufficient amount of anticoagulant to the selected components to prevent coagulation and/or flocculation. The methods and systems limit the amount of anticoagulant returned to the donor and maximize the amount of collected plasma. Methods and systems for maximize the collection of IgG based on a measured level of total plasma protein are also disclosed.