A system for extracting and processing adipose tissue to generate a therapeutically effective amount of adipose-derived stem cells, comprising an adipose tissue extraction device and a modified centrifuge tube comprising a plurality of lipoaspirate inlet fittings, a plurality of processing fluid inlet fittings, and a plurality of pellet extraction tubes. The adipose tissue extraction device is used to extract a quantity of adipose tissue from a human being, the lipoaspirate is moved into the first modified centrifuge tube via a sterile transfer, a plurality of processing steps are performed to clean and dissociate the lipoaspirate, and a pellet containing an enhanced fraction of stem cells is obtained by centrifuging the modified centrifugal tube. The pellet is resuspended in a fluid and administered to a human patient for a therapeutic or cosmetic purpose.

Some systems, devices and methods detailed herein provide fat harvesting from a body location, material screening, size selection, and extraction for fat preparation prior to reinjection into another body location.

A method of collecting mononuclear cells, comprising separating whole blood into plasma and cellular components, combining the cellular components with plasma replacement fluid to form a first mixture, and separating the first mixture into mononuclear cells and at least one component.

A screen exchange device for reducing the size of a biological tissue according to one embodiment comprises: a disk comprising a plurality of screens each having at least one through-hole configured to reduce the size of a biological tissue, the screens having different through-hole characteristics; a first cover which covers a first side of the disk and includes a first opening through which a biological tissue passes; a second cover which covers a second side of the disk and includes a second opening through which the biological tissue passes; a housing configured to receive the disk; and a manipulator which selects any one screen among the plurality of screens and manipulates the first cover or the second cover such that the selected screen communicates with the first opening and the second opening.

The present invention relates to a fat transplantation machine (1) comprising: a fat suction circuit, a filtration device (100) comprising a filter delimiting a centrifugation chamber (160) for an adipose tissue, the filter having a pore size configured to allow a liquid medium to pass and retain an adipose tissue, a rotational drive device (30) connected to the filtration device, a mass measurement device (5) configured to measure the mass of adipose tissue present in the filtration device, a processing unit (10) configured to control the rotational drive device (300) so as to initiate at least one centrifugation filtration sequence as a function of a predetermined mass of suctioned adipose tissue, the processing unit being further configured to calculate an injectable amount of adipose tissue (Q.sub.injectable) after each purification sequence.

A syringe for withdrawing an aspirate from a body for micro fat grafting includes a barrel having a first barrel portion with an inner wall with a lesser diameter than an inner wall of a second portion. A filter for separating fat cells from the aspirate extends within the second portion of the barrel. An absorbent is positioned between the filter and the second portion inner wall for absorbing the aspirate passing through the filter. A plunger moves within the barrel along the first portion inner wall and the filter and includes a gasket forming a seal with the first portion inner wall and an inner wall of the filter. Proximal and distal supports engage the filter and the barrel. In another embodiment, the filter has flared ends for engaging the barrel. A method of obtaining fat cells for micro fat grafting using the syringe is provided.

A gadget for use in harvesting and transfer of fat tissue, including a canister dimensioned to operate in absence of stirring (whether manual or mechanical) and filtering of lipoaspitate and to remove the unwanted fluid portion of lipoaspirate without the use of vacuum.