A method for cross-section processing and observation, and apparatus therefor, includes performing a position information obtaining process of observing the entirety of a sample by using an optical microscope or an electron microscope, and obtaining three-dimensional position coordinate information of a particular observation target object included in the sample; performing a cross-section processing process of irradiating a particular region in which the object is present by using a focused ion beam based on the information, and exposing a cross section of the region; performing a cross-section image obtaining process of irradiating the cross section by using an electron beam, and obtaining a cross-section image of a predetermined size region including the object; and performing a three-dimensional image obtaining process of repeating the cross-section processing process and the cross-section image obtaining process at predetermined intervals in a predetermined direction, and obtaining a three-dimensional image from the multiple cross-section images.

A method capable of constructing an accurate three-dimensional image is offered. The method comprises the step (S10) of obtaining a first series of tilted images which are constituted by electron microscope images or elemental mapping images of a sample (S) at different tilt angles and which have been obtained by tilting the sample in angular increments, the step (S14) of obtaining a second series of tilted images which are constituted by electron microscope images or elemental mapping images of the sample at different tilt angles and which have been obtained by rotating the sample about an axis (P) perpendicular to a surface (Sf) of the sample and then tilting the sample in angular increments, and the step (S16) of constructing the three-dimensional image on the basis of the first and second series of tilted images.

Devices and methods are described for performing high angle tilting tomography on samples in a liquid medium using transmission electron beam instruments.

A method of determining a depth of a hole milled into a first region of a sample, comprising: positioning the sample in a processing chamber having a charged particle beam column; milling a hole in the first region of the sample using a charged particle beam generated by the charged particle beam column; identifying a first registration mark at an upper level of the milled hole; identifying a second registration mark at a lower level of the milled hole; taking a first set of images at a first tilt angle, the first set of images including a first image taken with a field of view that captures the first registration mark but not the second registration mark, and a second image taken with a field of view that captures the second registration mark but not the first registration mark; taking a second set of images at a second tilt angle, different than the first tilt angle, the second set of images including a third image taken with a field of view that captures the first registration mark but not the second registration mark, and a fourth image taken with a field of view that captures the second registration mark but not the first registration mark; using stereoscopic measurement techniques to determine the depth of the hole based on the first and second sets of images.

The present application discloses methods, systems and devices for using charged particle beam tools to pattern and inspect a substrate. The inventors have discovered that it is highly advantageous to use patterns generated using the Hadamard transform as alignment and registration marks (Hadamard targets) for multiple-column charged particle beam substrate processing and inspection tools. Hadamard targets can be written to a substrate using charged particle beams performing, for example, resist-based lithography or resist-less direct processing. High-order Hadamard targets can also be patterned and imaged to obtain superior column performance metrics for applications such as super-rapid beam calibration DOE, column matching, and column performance tracking. Hadamard target blocks can be written highly locally to electrically functional pattern portions, or integrated into said pattern portions, thereby enabling re-registration local and contemporaneous to writing and improving beam targeting accuracy following re-registration. Superior alignment and registration, and column parameter optimization, allow significant yield gains.

A technique for estimating a whole object surface area and volume of a micro-scale three-dimensional model with a partially visible surface includes receiving a single-view stereoscopic image of an object of interest and an unconstrained three-dimensional point cloud of the object, generating a constrained three-dimensional point cloud using the image, the unconstrained three-dimensional point cloud, and a digital elevation model (DEM) of the object generated from the image, generating, using the constrained three-dimensional point cloud, a three-dimensional mesh representing an estimate of the surface of the object, calculating a partial surface area and/or partial volume of the object using the three-dimensional mesh, estimating an extent of a visible surface of the object, and calculating a whole surface area and/or a whole volume of the object based on the partial surface area of the object and the estimated extent of the visible surface of the object.

A cross-section processing and observation method performed by a cross-section processing and observation apparatus comprises a cross-section processing step of forming a cross-section by irradiating a sample with an ion beam; a cross-section observation step of obtaining an observation image of the cross-section by irradiating the cross-section with an electron beam; and repeating the cross-section processing step and the cross-section observation step so as to obtain observation images of a plurality of cross-sections. In a case where Energy Dispersive X-ray Spectrometry (EDS) measurement of the cross-section is performed and an X-ray of a specified material or of a non-specified material that is different from a pre-specified material is detected, an irradiation condition of the ion beam is changed so as to obtain observation images of a plurality of cross-sections of the specified material, and the cross-section processing and observation of the specified material is performed.

Methods of investigating a specimen using tomographic imaging include the following steps. A specimen is provided on a specimen holder and a beam of radiation is directed through the specimen and onto a detector, thereby generating an image of the specimen. The directing is repeated for a set of different specimen orientations relative to the beam, thereby generating a corresponding set of images. An iterative mathematical reconstruction technique is used to convert the set of images into a tomogram of at least a portion of the specimen. The reconstruction is mathematically constrained so as to curtail a solution space resulting therefrom. In addition, three-dimensional SEM imagery of at least a part of the specimen that overlaps at least partially with the portion is obtained. The three dimensional SEM imagery is used to perform the constraining step by requiring iterative results of the reconstruction to be consistent with pixel values derived from the SEM imagery

A method for manipulating an object for imaging by an imaging device includes the steps of rotating the object about a rotation axis into a plurality of angular positions; capturing an image of the object at each of the plurality of angular positions; and determining a respective translation required of the object for the plurality of angular positions, the translation being along a plane substantially orthogonal to the rotation axis; wherein the respective translation is arranged to align the object to the rotation axis so as to maintain the object within a field of view of the imaging device.

A method for manipulating an object for imaging by an imaging device includes the steps of rotating the object about a rotation axis into a plurality of angular positions; capturing an image of the object at each of the plurality of angular positions; and determining a respective translation required of the object for the plurality of angular positions, the translation being along a plane substantially orthogonal to the rotation axis; wherein the respective translation is arranged to align the object to the rotation axis so as to maintain the object within a field of view of the imaging device.