Compositions and methods for separating a sample of whole blood or bone marrow aspirate into a fraction rich in at least one of platelets and pluripotent cells are provided. A sample of whole blood can be centrifuged in a collection tube comprising a separator substance formulated to settle between the PRP fraction and the at least one other fraction. Preferably, centrifugation is completed without substantial activation of platelets. Optionally, the separator substance could be hardened to form a solid barrier that allows removal of all or substantially all of the platelets in the PRP fraction without remixing of the PRP fraction with the at least one other fraction. Transfer devices and methods are also provided in which a sterile sample can be transferred from a separation/preparation container (e.g., vacutainer) to a consumer or other container (e.g., dropper) while maintaining sterility of the sample.

A portable device for removal of metabolic waste from the blood of patient having kidney disease or in need of hemodialysis is provided. Methods of hemodialysis employing the portable device beneficially obtain a dialysate by electrokinetic means from excess fluid in the peripheral blood of the patient in need thereof. The methods employ a branched microfluidic channel for the use of ion concentration polarization to separate charged from neutral species in blood to obtain the dialysate for undergoing hemodialysis. Beneficially the methods and device are resistant to biofouling, remove the need for a dialysate and/or dialysate reservoir, and provide a disposable, wearable device.

An apheresis system includes a chamber configured to receive a centrifuge assembly and a moving loop holder. The moving loop holder includes a loop holder that has a loop connection that is configured to interact with a flexible loop received by the centrifuge assembly. The moving loop holder is configured to move between a first position and a second position, where in the first position, the moving loop holder is a first distance from the centrifuge assembly, and in the second position, the moving loop holder is a second distance from the centrifuge assembly. The centrifuge assembly is prevented from moving from an operating state to a loading state when the moving loop holder is in the first position, and the centrifuge assembly is allowed to move from the operating state to the loading state when the moving loop holder is in the second position.

A method includes initiating one or more pumps of an apheresis machine, detecting a flow of fluid through the apheresis machine, detecting the flow of fluid is below a predetermined threshold, and in response to detecting the flow of fluid is below the predetermined threshold, adjusting a rate of the one or more pumps of the apheresis machine.

The invention provides apheresis devices and their use for removal of substantially all types of cell free DNA (cfDNA) in patients' blood, including nucleosome-bound cfDNA, exosome-bound cfDNA and unbound cfDNA (including double stranded DNA (dsDNA), single stranded DNA (ssDNA) and oligonucleotides), to limit the negative effects of the circulating cfDNA and to treat various diseases.

A load cell assembly includes a load cell and a deflection portion. The load cell includes a fixed end and a free end. The free end is configured to receive a force in a first direction. The deflection portion includes a first component and a second component. The first component is coupled to the free end of the load cell. The first component defines a first cam surface. The second component is configured to support the vessel. The second component defines a second cam surface. The second cam surface is configured to engage the first cam surface. In an engaged state, the second cam surface is in continuous direct contact with the first cam surface. In a disengaged state, at least a portion of the second cam surface is disengaged from the first cam surface. The load cell assembly includes a central axis.

A collection bottle includes a lid and a canister. The canister has a closed end and an opened end and defines an interior space. The lid includes a lid body having a first side and a second side, a shield handle, and one or more ports. The first side of the lid body is configured to be coupled to the open end of the body to close the interior space. The shield handle and the one or more ports extend from the second side of the lid body. The shield handle extends a first distance from the second side of the lid body. The one or more ports extend a second distance from the second side of the lid body. The first distance is greater than the first distance.

A separation assembly for an apheresis system includes a first media bag, a second media mag, a vessel, and a separation set. The first media bag contains a first fluid medium. The second media bag contains a second fluid medium. The vessel is configured to contain a third fluid medium. The separation set includes a first tube and a second tube. The first tube has a first fitting that is configured to be coupled to the first media bag. The first tube defines a first length. The second tube has a second fitting that is configured to be coupled to the second media bag. The second tube defines a second length different from the first length. The separation set may further includes a third tube is configured to be coupled to the vessel.

A method includes receiving, with an apheresis system, data associated with a donor; determining, based on the data associated with a donor, an identification of the donor; receiving, with the apheresis system, data associated with a blood component collection set; receiving, with the apheresis system, data associated with a plasma collection bottle; and performing, with the apheresis system, a plasma donation process based on the identification of the donor, the data associated with the blood component set, and the data associated with the plasma collection bottle. Receiving the data associated with the donor may include scanning, with a scanner, an image. The scanner is disposed on the apheresis system and the image may include one of a one-dimensional barcode or a two-dimensional barcode. The data associated with the donor may be displayed on a user device.

A soft cassette includes a body including a flexible material and first and second component. The body defines first and second lumens and first and second chambers. The first lumen extends between first and second ends. The first chamber is in the first lumen such that fluid passing through the first lumen passes through the first chamber. The second lumen is fluidly connected to the first lumen at a first junction between the first end and the first chamber and a second junction between the second end and the first chamber such that fluid passing through the second lumen bypasses the first chamber. The second chamber is in the second lumen. The first component is on a first side of the second chamber and includes a first ferromagnetic metal or magnet. The second component is on a second side of the second chamber and includes a second ferromagnetic metal magnet.