A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

A centrifugal device includes a container having a body with a first end and a second end disposed opposite to the first end. A cap is coupled to the second end of the container, and the cap includes a top surface having at least one port configured to receive or transmit one or more of air or fluid. So configured, the container is moveable between an upright position, in which a first fluid disposed in the container is centrifuged to precipitate at least one extracellular vesicle separate from the first fluid, and an inverted position in which one or more of the first fluid having at least one extracellular vesicle depleted therefrom is removed from the container and a second fluid mixed with the at least one extracellular vesicle removed is withdrawn from the container for injection.

The present invention may include an upper cap having an opening/closing member for opening and closing an inlet through which a blood is injected; a main body housing having an open upper end outer surface thereof assembled with the upper cap, and having an inner space in which the blood is filled with; an adjustment housing that is coupled to the main body housing to seal the open lower end of the main body housing, and is movably assembled with respect to the main body housing so that a buffy coat layer is positioned on a neck portion; and a side cap fixed to the neck portion by forming a needle hole through which an end of an injection needle for extraction enters and exits in area corresponding to a sealing member to seal an extraction hole formed through the neck portion.

Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.

This invention relates in general to the field of cell-therapy treatments and more particularly, but not by way of limitation, to systems and methods for administering personalized cell-therapy treatments intravenously. In various embodiments, the system may calculate an aspiration volume needed for centrifugation to achieve a concentrated target threshold dose of 2×10.sup.6 platelets/μL for a particular cell therapy using various factors such as, for example, information about a patient and the efficiency of the concentration process.

The invention provides apheresis devices and their use for substantial removal of all types of cfDNA for treatment of various diseases and during perfusion of an organ and/or anatomical cavity, to limit the negative effects of circulating cfDNA during organ transplantation and thus improve the quality and survival of transplanted organs, and reduce unfavorable transplantation outcomes such as transplant dysfunction, ischemia-reperfusion injury, graft rejection, and organ failure.

The disclosure provides for a container system and method for the cryopreservation and resuspension of a body fluid. The container system may include a cryopreservation container comprising the body fluid in a cryopreservation liquid, a resuspension container comprising a resuspension solution, a connection tube sterilely connecting the cryopreservation container and the resuspension container, and at least one shut-off element actively associated with the connection tube.

A processing system includes a processor including a separator, a set configured to cooperate with the separator to separate whole blood into plasma and other components, the set including an inlet line attachable to a patient to receive whole blood and an return line attachable to a patient to return processed fluid, and a source of replacement fluid connected to the disposable set, the processor configured to combine the other components with replacement fluid to define the processed fluid. The processor includes a controller and an input device coupled to the controller, the controller configured to receive an input via the input device, the input representing a volume of replacement fluid, and to control the processor to separate whole blood passing through the set and to combine the other components with the replacement fluid according to the input until the source of replacement fluid is empty.

A method of collecting plasma includes receiving donor parameters at a controller of a plasma collection device electronically from a donor management system. The method includes storing a target volume for raw plasma which is based at least in part on donor height and weight used to calculate total donor blood volume, the target volume for raw plasma based on the total donor blood volume. The method includes setting the target volume for raw plasma and controlling the plasma collection device to operate draw and return phases to withdraw whole blood from a donor and separate the whole blood into the plasma product and a second blood component comprising red blood cells and to return the second blood component to the donor. The controller operates the draw and return phases until a volume of raw plasma in the collection container equals the target volume of raw plasma.

A fluid processing device includes a controller, a centrifuge, and a pump system. The controller controls the pump system to convey a fluid into a centrifuge chamber received by the centrifuge at first and second rates, with the controller also controlling the centrifuge to rotate the chamber at a first rotation rate when the fluid is being conveyed into the chamber at the first rate and controlling the centrifuge to rotate the chamber at a second rotation rate when the fluid is being conveyed into the chamber at the second rate. The first and second rotation rates are different, with each being based at least in part on a concentration of a fluid component within the fluid, the rate at which the pump system is conveying the fluid into the centrifuge chamber, and a target concentration of the fluid component in one of the first and second constituents.