A syringe holder for holding a syringe in a centrifuge, the syringe having a plunger-end and a nipple-end, the syringe holder holding the syringe nipple-end upwardly, comprising: a cup having a cup seat, a wall, a bore, a mouth, and a support, the support supporting the cup in the centrifuge; a lower support having a upper surface and a lower surface, the lower support sized to slide into the cup and the lower support supporting the plunger-end of the syringe; an upper support having lower section and an upper section with a lip, and having a channel therethrough, the lip having a outer diameter larger than the inner diameter of the mouth of the cup, the channel therethrough co-axial with the lower support and the bore of the cup, the lower section having an outer diameter that is less than the inner diameter of the bore of the of the cup and sized to permit the upper support to slide at least partially into the cup and the lip to contact the mouth of the cup and support the upper support in the cup; whereby the upper support and the lower support support the syringe with the needle-end upwardly in the cup during centrifuging.

Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.

A method for automated processing of a blood product, the method comprising providing a reusable separation apparatus controlled by a microprocessing unit, said apparatus configurable with settings and configured to associate with a disposable circuit comprising a separator and in communication with a source blood product having a first concentration and first volume. The apparatus and disposable circuit are configured to flow the source blood product into an inlet of the separator and separate supernatant of the source blood product from a first outlet of the separator into a filtrate container. The apparatus and disposable circuit are also configured to separate platelets and remaining supernatant from a second outlet of the separator into a retentate container, wherein the platelets and remaining supernatant in the retentate container have a second concentration greater than the first concentration and second volume less than the first volume.

The present invention relates to a cytopheretic cartridge for use in treating and/or preventing inflammatory conditions that affect myocardial function and to related methods. The cartridge can be used in treating a subject with myocardial dysfunction, such as a subject with chronic heart failure and/or acute decompensated heart failure.

Methods of preparing liquid exchanged biological fractions are provided. Aspects of the present disclosure according to certain embodiments include introducing a biological sample into a centrifugation container having a buoy, subjecting the biological sample to a force of centrifugation to produce two or more fractions in the biological sample, collecting a first fraction of the biological sample from the container and introducing a liquid exchange fluid into the container to produce a liquid exchanged fraction that is a mixture of the liquid exchange fluid and a second fraction of the biological sample. Compositions having a liquid exchanged platelet-rich fluid and kits for practicing embodiments of the subject methods are also provided. Methods for applying compositions having a liquid exchanged platelet-rich fluid to a body site of the subject are described.

In the presented invention, by using two connected polymer bags and the connection to the drawing blood, the first separation in the first bag and transmission of the blood components; such as RPP into the second bag and re-injecting the remaining blood to the considered person are possible.

A washed platelet having a sufficiently low blood plasma content rate is more securely and efficiently obtained.

A tertiary separator (42) includes a main body (58) which has a third chamber (52) and is formed as an accommodating portion (54a) accommodating a centrifuged platelet (104), an inlet (77c) which allows a platelet containing component (100) and a platelet added solution (102) to flow in, and an outlet (78a) which allows blood plasma, the platelet added solution (102), and the platelet (104) to flow out. A bottom portion (first bottom portion (60)) of at least a portion forming the accommodating portion (54a) in a wall portion included in the main body (58) is formed of a soft material.

A blood separation method is provided. The blood separation method includes the following steps. Firstly, a whole blood is placed in a container having a separating gel, which includes a silicon-containing polymer, and a specific gravity of the separating gel is between 1.030 and 1.093. Then, a centrifugation is performed at a first effective rotation speed to divide the whole blood into a red blood cell layer located below the separating gel, a buffy coat layer located above the separating gel, and a plasma layer located above the buffy coat layer. The buffy coat layer and the plasma layer are then mixed to obtain a platelet-rich plasma.

Systems and method for verifying the timely placement of a container of biolocal cells or fluid within a treatment chamber of a biological fluid treating device are disclosed. The systems and methods utilize a sensor that detects the presence of a container at an appropriate and pre-determined time and, optionally, detects the weight of the container and cells.

Provided is a method for producing platelets, in which damage to platelets is suppressed compared with a method in which platelets are separated using a filter from a megakaryocyte culture, and then the platelets are concentrated using a hollow fiber membrane and are further washed using the hollow fiber membrane, and purified platelets can be produced in a shorter period of time compared with the time that is taken to perform the above-described method so as to reduce damage to platelets. The method for producing purified platelets of the present invention includes a concentrating step of concentrating a megakaryocyte culture, and a centrifuging step of centrifuging platelets from an obtained concentrate.