A blood separation method using a blood separation filter (10A) includes: an arrangement step of arranging a housing (18) such that a blood inflow chamber (20) is positioned below a filter member (24) and the blood outflow chamber (22) is positioned above a filter medium (38); a blood treatment step of allowing blood to flow in the filter medium (38) upward from vertically below; and a post residual treatment blood collection step of arranging the housing (18) such that an outflow port (28) is positioned vertically below the blood outflow chamber (22) so as to guide the post-separation residual blood in the housing (18) to the outflow port (28).

A platelet incubator usable as a lid for a refrigerated container includes a heating plate and a transparent cover. The heating plate has an aluminum top plate, an aluminum middle plate with an opening into which a silicon mat heater is placed, and a 5052 aluminum alloy bottom plate, with an aperture through which the wires providing electrical energy to the silicon mat heater are placed. The temperature is continuously monitored, and a log is kept for printout, allowing the platelet incubator to meet relevant compliance requirements. An alarm is activated if the temperature of the platelets exceeds twenty-three degrees Celsius, or falls under twenty-one degrees Celsius.

A minimally invasive cosmetic procedure for de-bulking fat includes steps of: (a) injecting platelet rich plasma (PRP) into a subject area; followed by (b) heating fat cells in the subject area using radiofrequency; followed by (c) aspirating fat cells in the subject area using liposuction; followed by (d) injecting platelet rich plasma (PRP) into the subject area again. The PRP injecting step (a) may be performed two to seven weeks prior to the heating and liposuction steps (b) and (c); and the PRP injecting step (d) may be performed several days to four weeks after the heating and liposuction steps (b) and (c).

A solid fiber is described, where the solid fiber is characterized by (a) a modification degree Do/Di, in a cross section of the solid fiber of 1.20 to 8.50 where the inscribed circle diameter is denoted by Di and the circumscribed circle diameter is denoted by Do; and (b) a porous specific surface area of not less than 30 m.sup.2/g.

An object of the present invention is to provide a material which can remove an activated leukocyte-activated platelet complex with high efficiency. The present invention provides a material for removing an activated leukocyte-activated platelet complex, the material being a water-insoluble carrier to the surface of which carrier a compound(s) having a charged functional group(s) is(are) bound, wherein an extending length ratio of the surface is 4 to 7.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

The present invention relates to a platelet rich plasma (PRP) extracting device and extracting method using the same, and more particularly, to a platelet rich plasma (PRP) extracting device capable of quickly and effectively extracting the highly concentrated PRP from centrifuged blood by a simple manipulation.

The present invention discloses a microstructured discrimination device for separating hydrophobic-hydrophilic fluidic composites comprising particulate and/or fluids in a fluid flow. The discrimination is the result of surface energy gradients obtained by physically varying a textured surface and/or by varying surface chemical properties, both of which are spatially graded. Such surfaces discriminate and spatially separate particulate and/or fluids without external energy input. The device of the present invention comprises a platform having bifurcating microchannels arranged radially. The lumenal surfaces of the microchannels may have a surface energy gradient created by varying the periodicity of hierarchically arranged microstructures along a dimension. The surface energy gradient is varied in two regions. In one pre-bifurcation region the surface energy gradient generates a fluid flow. In the other post-bifurcation region, there is a difference in surface energy proximal to the bifurcation such that different flow fractions are divided into separate channels in response to different surface energy gradients in each of the post-bifurcation channels. Accordingly, fluids of different hydrophobicity and/or particulate of different hydrophobicity are driven into separate channels by a global minimization of the fluid system energy.

A centrifugal device includes a container having a body with a first end and a second end disposed opposite to the first end. A cap is coupled to the second end of the container, and the cap includes a top surface having at least one port configured to receive or transmit one or more of air or fluid. So configured, the container is moveable between an upright position, in which a first fluid disposed in the container is centrifuged to precipitate at least one extracellular vesicle separate from the first fluid, and an inverted position in which one or more of the first fluid having at least one extracellular vesicle depleted therefrom is removed from the container and a second fluid mixed with the at least one extracellular vesicle removed is withdrawn from the container for injection.

A blood component separation device for separating a plurality of blood components from blood sampled from a blood donor, and collecting platelets, includes: an operation unit that calculates a predicted platelet recovery rate from a hematocrit value of the blood and a platelet concentration of the blood, and calculates a recommended processing amount of the blood recommended for collecting a target number of units of platelets on the basis of the calculated predicted platelet recovery rate, wherein the operating unit sets the predicted platelet recovery rate calculated from any the hematocrit value and any the platelet concentration to be smaller by a predetermined value a when the blood donor is female than that when the blood donor is male.