The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.

A blood component separation device includes a centrifuge bowl for separating a blood component from blood, a plasma bag for storing a plasma component, a platelet intermediate bag for storing high-concentration platelet liquid having high-concentration of platelets, and a temporary storage bag (also used as a buffy coat bag) for storing low-concentration platelet liquid having low-concentration of platelets. The blood component separation device performs control, from the second cycle onward, to mix the low-concentration platelet liquid stored in the temporary storage bag in the immediately preceding cycle with whole blood to supply the mixed liquid to the centrifuge bowl. An amount of high-concentration platelet liquid to be collected in the platelet intermediate bag in the first cycle is set to be smallest among all cycles, and an amount of high-concentration platelet liquid to be collected in a last cycle is set to be greatest among all the cycles.

Provided are methods for preparing pathogen-inactivated platelet compositions, as well as processing sets and compositions related thereto.

Provided is a blood processing filter comprising a container having two spouts serving as an inlet for a liquid to be processed and an outlet for the processed liquid, and a filtration medium contained in the container, the filtration medium comprising a filter material having different CWST values for one surface A and the other surface B.

An integrated kit for separating blood and concentrating PRP includes: (a) a main body that includes an upper storage portion, a lower storage portion, and a passage portion for connecting the upper storage portion and the lower storage portion; (b) a upper cover coupled to an upper end of the upper storage portion; (c) an inner stopper inserted into a lower end of the lower storage portion so as to seal the lower storage portion; (d) a closing adjustment screw which can move vertically while penetrating a hole formed at the inner stopper and seals the passage portion; and (e) a lower cover coupled to an outside of the lower end of the lower storage portion and the outside of the inner stopper to prevent the separation of the closing adjustment screw coupled to the inner stopper in case of centrifugation.

Embodiments provide methods and apparatus for fabricating blood products, such as platelet-rich plasma, containing elevated concentrations of growth factors such as platelet derived growth factor. The platelet-rich plasma can be autologous, and the concentration of growth factors (e.g., platelet derived growth factor) is elevated relative to other samples isolated from the same subject. The platelet-rich plasma can be used to promote tissue regeneration, including wound healing, joint repair, hair growth, and the like. The compositions can be combined with stem cells and used to treat disorders otherwise treated with stem cells. The growth factors present in the samples can direct the differentiation of the stem cells.

A tray for processing platelet rich fibrin includes a base having at least one alignment structure, a screen attachment having at least one alignment structure for engagement with the base, and a lid having at least one alignment structure for engagement with the base and the screen attachment. The screen attachment includes a screen offset between the top and bottom surface of the screen. When the screen attachment is placed on the base with the top surface facing upward, the lid is configured to compress a fibrin clot placed on the screen to a first thickness, and when the screen attachment is placed on the base with the bottom surface facing upward the lid is configured to compress a fibrin clot placed on the screen to a second thickness different from the first thickness.

Embodiments are described for separating/collecting components from a multi-component fluid such as whole blood. Some embodiments provide for controlling the amount of a component, such as platelets, introduced into a separation chamber to ensure that the density of fluid in the separation chamber does not exceed a particular value. This may provide for collecting purer components. Other embodiments may provide for determining a chamber flow rate based on a concentration of a component in the multi-component fluid, which may then be used to determine a centrifuge speed, to collect purer concentrated components.

The present invention relates to a cytopheretic cartridge for use in treating and/or preventing inflammatory conditions within a subject and to related methods. More particularly, the invention relates to a cytopheretic cartridge that includes a housing and, disposed within the housing, a solid support capable of sequestering activated leukocytes and/or platelets.

Platelet rich plasma containing human very small embryonic-like stem cells (hVSEL) is treated with amplitude-modulated pulses of laser light having a predefined wavelength for a predefined time period, where the predefined wavelength ranges from 300 nm to 1000 nm. Treatment of the platelet rich plasma using this method results in an unexpectedly high degree of proliferation of the hVSEL in the platelet rich plasma, resulting in reduction of biological age, when administered to a patient.