Provided herein are systems and methods for molecule delivery into cells. The method may include steps of forming a solution comprising an amount of microbubbles, an amount of soluble molecules, and one or more cells, flowing the solution through a microfluidic device, and sonicating the solution for a period of time sufficient to form pores in the one or more cells. In particular, such methods may be used for preservation of cells through delivery of a preservation agent.

The present invention discloses a microstructured discrimination device for separating hydrophobic-hydrophilic fluidic composites comprising particulate and/or fluids in a fluid flow. The discrimination is the result of surface energy gradients obtained by physically varying a textured surface and/or by varying surface chemical properties, both of which are spatially graded. Such surfaces discriminate and spatially separate particulate and/or fluids without external energy input. The device of the present invention comprises a platform having bifurcating microchannels arranged radially. The lumenal surfaces of the microchannels may have a surface energy gradient created by varying the periodicity of hierarchically arranged microstructures along a dimension. The surface energy gradient is varied in two regions. In one pre-bifurcation region the surface energy gradient generates a fluid flow. In the other post-bifurcation region, there is a difference in surface energy proximal to the bifurcation such that different flow fractions are divided into separate channels in response to different surface energy gradients in each of the post-bifurcation channels. Accordingly, fluids of different hydrophobicity and/or particulate of different hydrophobicity are driven into separate channels by a global minimization of the fluid system energy.

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.

A third cassette (28) of a blood component collection kit (12) is equipped with a third cassette main body (29) in which a flow path (F) is formed together with having a filter accommodating unit (86), and a filter member (88) disposed in the filter accommodating unit (86). The flow path (F) includes a red blood cell line (f1) in which red blood cells flow, a preservation solution line (f2) in which a preservation solution flows, a confluence section (f3) in which the red blood cells and a preservation solution are made to merge together, a mixed liquid line (f4) through which a mixed liquid of the red blood cells and the preservation solution flows, and a filter chamber (f5) formed inside the filter accommodating unit (86).

A blood washing system including a rotor defining an internal chamber and a skimmer assembly configured to move a withdrawal needle within the internal chamber. A multi-component fluid, such as a whole blood sample, can be fed into the internal chamber via a feed tube, where the rotor can be rotated to fractionate the multi-component fluid. A brake can be applied to the rotor to cease rotation or rotated at a slower speed to allow the fractions of the multi-component to settle on a bottom wall of the rotor. The withdrawal needle is moveable within the internal chamber to align an orifice of the withdrawal needle for withdrawing the liquid fractions and isolate the solid fractions. Wash fluids can be added to the internal chamber to repeat the wash cycle without removing the solid fractions. The washed solid fractions can be withdrawn via the feed tube and collected.

A disposable bag set for separating discrete volumes of a composite fluid includes a separation bag including the composite fluid to be separated; a first bag in fluid communication with the separation bag, the first bag configured to receive a first component of the composite fluid from the separation bag; and a second bag in fluid communication with the first bag and not the separation bag, the second bag configured to receive a product formed using the first component.

Apparatus for treating blood. A device (10) for separating plasma from blood comprises a blood flow path (20) including formations (24) for agitating blood flow along the blood flow path and a separation membrane (28) with a first surface in fluid communication with the blood flow path. The device may be comprised in an extracorporeal blood circuit and used in a method for separating plasma from blood.

A method and automated system for processing blood in which the automated system includes a programmable controller, a database, and an interactive display screen for displaying information and receiving operator input. The programmable controller is configured to automatically control the system to perform the method. Upon activation of the system, the screen displays a listing of different blood processing procedures that may be performed using the system. The operator may then input into the controller an identification of a specified blood processing procedure that is to be performed, such that an initial list of parameters that are associated with the specified blood processing procedure are displayed on the screen. The operator may then input into the controller an identification of the parameters that are to populate the display screen during performance of the procedure and indicate a format in which the selected parameters are to be presented on the display screen. The controller then creates a display for the specified blood processing procedure. Current values of the selected parameters in the selected format are displayed on the screen during performance of the specified procedure. The controller automatically saves an image of the display screen periodically during performance of the specified blood processing procedure, and transfers information from the saved images of the display screens to a procedure record form.

Medical containers and disposable fluid circuits (kits) including such containers are disclosed. The containers and kit components are made of a plastic composition including polyvinyl chloride and one or more plasticizers such as a citrate ester and an epoxidized vegetable oil are disclosed. Containers made from such compositions are useful in the storage red blood cells. Red blood cell products wherein the red blood cells exhibit a reduced level of hemolysis are also disclosed.

A dialyzer control apparatus includes a magnetic field generator which is disposed on an outer surface of a dialyzer which removes wastes from a blood flowing therein and generates a magnetic field; and a controller which controls the magnetic field generator to perform a magnetic field stimulation on the blood flowing into the dialyzer. The magnetic field stimulation may be a stimulation related to the improvement of the Rouleaux formation for the red blood cells in the blood.