The invention relates to a method for separating blood, to a separation container and to a system, wherein different blood fractionserythrocytes (64), buffy coat (65) and blood plasma (66)are obtained, wherein blood is introduced into a separation container (1) and is then centrifuged into different superimposed, fluidically connected sections of the separation container, specifically a top section (4) for receiving the blood plasma (66), a middle section (3) for receiving the buffy coat, and a bottom section (2) for receiving the erythrocytes (64). The aim of the invention is to optimize the extraction of buffy coat. Said aim is achieved by means of the method and the separation container, wherein the optimization is geared to obtaining a defined phase boundary, and by means of the system, wherein the optimization is geared to achieving freedom from contamination from the time the blood enters the separation container and obtaining an easy-to-handle freezer container for cryopreservation. By means of the haematocrit of the blood supplied, the future packing volume of the erythrocytes (64) to be centrifuged is determined. Furthermore, the capacity of the bottom section (2) is adjusted to the expected packing volume of the erythrocytes after the centrifuging in such a manner that the phase boundary (5) forming during centrifuging between buffy coat (65) and erythrocytes (64) is positioned in a region of the middle section (3) of the separating container (1) adjacent to the bottom section (2), and finally the amount of blood supplied is introduced in the exact volume into the separation container (1), taking into account the expected packing volume of the erythrocytes (64). The separation container (1) enables the method to be carried out. The system is kept closed and avoids interaction with the environment.

The present invention relates to a biomedical device for the production, storage, traceability and administration of blood components.

Described herein are systems, devices, and methods for an extracorporeal, artificial, placenta. In some embodiments, an artificial placenta and amniotic bed system may comprise a control unit, a gas delivery unit, a gas exchange unit or membrane oxygenator, a fluids delivery unit, an amniotic fluid bed, and a human machine interface. In some embodiments, the artificial placenta and amniotic bed systems, devices, and methods described herein may improve survival rates and minimize long-term disabilities in preterm, gestational-age, newborns. In some embodiments, the extracorporeal systems, devices, and methods comprise an artificial network through which oxygen and nutrient-rich blood may flow into a fetus (residing in an amniotic fluid bed), while carbon dioxide and wastes may be removed, thus re-establishing a form of intrauterine placental circulation.

Disclosed is a system for the collection, processing and reuse of amniotic fluid and placental aspirate at a C-section site. The system includes a canister positioned along the vacuum line through which the amniotic fluid and placental aspirate is suctioned. The canister has a coil whereby the heavier cellular components, including stem cells, platelets and growth factors, are separated coincident with the surgical procedure. The canister has a port whereby the heavier cellular material can be removed from the canister. The heavier cellular material can be then applied to the wound site of the cesarean section patient. The system disclosed allows for the processing of the amniotic fluid and placental aspirate to take place in the same room as the surgical procedure. A kit and method are also provided.

The present disclosure provides an apparatus, and methods of using same, for collecting blood from an umbilical cord in a sterile environment that includes an umbilical cord retaining assembly and a blood collection assembly rotatably connected to the umbilical cord retaining assembly. A cord cutting blade, which is carried by the blood collection assembly, functions to cut the umbilical cord when the blood collection assembly is rotated relative to the umbilical cord retaining assembly. After the umbilical cord is cut, blood flows by force of gravity into the blood collection region of the blood collection assembly to which a blood collection bag can be interconnected.

A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating the embryonic-like cells from the residual cells and perfusion liquid. Exogenous cells can be propagated in the placental bioreactor and bioactive molecules collected therefrom.

A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

The present disclosure provides an apparatus, and methods of using same, for collecting blood from an umbilical cord in a sterile environment that includes an umbilical cord retaining assembly and a blood collection assembly rotatably connected to the umbilical cord retaining assembly. A cord cutting blade, which is carried by the blood collection assembly, functions to cut the umbilical cord when the blood collection assembly is rotated relative to the umbilical cord retaining assembly. After the umbilical cord is cut, blood flows by force of gravity into the blood collection region of the blood collection assembly to which a blood collection bag can be interconnected.

Disclosed is a system for the collection, processing and reuse of amniotic fluid and placental aspirate at a C-section site. The system includes a canister positioned along the vacuum line through which the amniotic fluid and placental aspirate is suctioned. The canister has a coil whereby the heavier cellular components, including stem cells, platelets and growth factors, are separated coincident with the surgical procedure. The canister has a port whereby the heavier cellular material can be removed from the canister. The heavier cellular material can be then applied to the wound site of the cesarean section patient. The system disclosed allows for the processing of the amniotic fluid and placental aspirate to take place in the same room as the surgical procedure. A kit and method are also provided.

The invention relates to a system and method for obtaining a cellular fraction enriched with defined cells effective in promoting a defined cellular response from an in vivo cellular sample. The system is closable to the atmosphere, and includes a cell suspension collection tube containing gel and anticoagulant, adapted such that, when containing cell suspension and centrifuged after treatment the collection tube yields separation fractions, a first fraction comprising cellular fluid, a second fraction comprising gel layer, a third fraction comprising enriched cellular portion on top of the gel layer and a fourth fraction comprising a cell poor portion. The cell poor portion may be extracted and discarded prior to extracting the enriched cellular portion.