Pulsed fluorescence imaging without input clock or data transmission clock is disclosed. A system includes an emitter for emitting pulses of electromagnetic radiation and an image sensor comprising a pixel array for sensing reflected electromagnetic radiation. The system includes a plurality of bidirectional data pads and a controller in communication with the image sensor. The system is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises one or more of: electromagnetic radiation having a wavelength from about 770 nm to about 790 nm.

Apparatuses, systems and methods for generating color video with a monochrome sensor include the acts of (i) selectively energizing each of a plurality of light sources in a sequence, (ii) capturing a monochrome image of the illuminated sample at a monochrome sensor at each stage of the sequence, and (iii) generating a color video from the monochrome images. The sequence can have a series of stages with each stage of the sequence corresponding to activation of a different wavelength of light from the light sources to illuminate a sample. Generating the monochrome video can include the acts of compiling a plurality of monochrome images captured at the monochrome sensor with a single light source into a series of monochrome video frames comprising the monochrome video.

Hyperspectral, fluorescence, and laser mapping imaging with reduced fixed pattern noise are disclosed. A method includes actuating an emitter to emit a plurality of pulses of electromagnetic radiation and sensing reflected electromagnetic radiation resulting from the plurality of pulses of electromagnetic radiation with a pixel array of an image sensor to generate a plurality of exposure frames. The method includes applying edge enhancement to edges within an exposure frame of the plurality of exposure frames. The method is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises one or more of: electromagnetic radiation having a wavelength from about 513 nm to about 545 nm, from about 565 nm to about 585 nm, from about 900 nm to about 1000 nm, an excitation wavelength of electromagnetic radiation that causes a reagent to fluoresce, or a laser mapping pattern.

Super resolution and color motion artifact correction in a pulsed hyperspectral, fluorescence, and laser mapping imaging system. A method includes actuating an emitter to emit pulses of electromagnetic radiation and sensing reflected electromagnetic radiation with a pixel array of an image sensor. The method includes detecting motion across two or more sequential exposure frames, compensating for the detected motion, and combining the two or more sequential exposure frames to generate an image frame. The method is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises one or more of: electromagnetic radiation having a wavelength from about 513 nm to about 545 nm, from about 565 nm to about 585 nm, from about 900 nm to about 1000 nm, an excitation wavelength of electromagnetic radiation that causes a reagent to fluoresce, or a laser mapping pattern.

Introduced here are light sources for flash photography configured to produce high-fidelity white light that is tunable over a broader range of correlated color temperatures (CCTs) than conventional flash technologies. The light source can include multiple independently controllable color channels representing illuminants (e.g., light-emitting diodes) of different colors with varying degrees of saturation. Operating collectively, the multiple color channels can produce a high spectral quality white light corresponding to different CCTs (e.g., “warm” white light having a red hue, “cool” white light having a blue hue). Operating independently, these same color channels can be pre-flashed in a variety of prescribed sequences to probe the spectral characteristics of a scene, thereby allowing for an enhanced, spectrally matched white flash as well as collecting per-pixel reflectivity data that can be later used in during post processing of the captured image.

Introduced here are computer programs and associated computer-implemented techniques for determining reflectance of an image on a per-pixel basis. More specifically, a characterization module can initially acquire a first data set generated by a multi-channel light source and a second data set generated by a multi-channel image sensor. The first data set may specify the illuminance of each channel of the multi-channel light source (which may be able to produce visible light and/or non-visible light), while the second data set may specify the response of each sensor channel of the multi-channel image sensor (which is configured to capture an image in conjunction with the light). Thus, the characterization module may determine reflectance based on illuminance and sensor response. The characterization module may also be configured to determine illuminance based on reflectance and sensor response, or determine sensor response based on illuminance and reflectance.

The present invention relates to a multi-band optical filtering method and apparatus and to a multi-band optical filtering method and apparatus capable of filtering wavelengths of two or more bands in order to create a multi-wavelength image of a subject. In the present invention, the optical filtering apparatus for creating a multi-wavelength image of a subject comprises a filter unit having a plurality of sub filter units comprising a first sub filter unit through which a first wavelength band passes and a second sub filter unit through which a second wavelength band, which is different from the first wavelength band, passes, wherein while the light generated in a light source passes through the filter unit, the filter unit filters the light such that an intensity of the first wavelength band is dominant in a first region, and an intensity of the second wavelength band is dominant in a second region.

The invention relates to a method allowing the use of the information accessible by fluorescence imaging to be optimized. For this purpose, it implements the combination of a protocol for calibration and synchronization of a pulsed light for exciting a fluorescent marker, with the operation in “rolling shutter” mode of a fluorescence camera. An appropriate correction factor allows the complete signal integrated by all of the photodiodes of the camera to be used so that no image is lost.

Hyperspectral, fluorescence, and laser mapping imaging with increased dynamic range is disclosed. A system includes an emitter for emitting pulses of electromagnetic radiation and an image sensor for sensing reflected electromagnetic radiation, wherein the pixel array comprises a plurality of pixels each configurable as a short exposure pixel or a long exposure pixel. The system is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises one or more of: electromagnetic radiation having a wavelength from about 513 nm to about 545 nm, from about 565 nm to about 585 nm, from about 900 nm to about 1000 nm, an excitation wavelength of electromagnetic radiation that causes a reagent to fluoresce, or a laser mapping pattern.

Controlling integral energy of a light pulse in a fluorescence imaging system is disclosed. A system includes an emitter for emitting pulses of electromagnetic radiation and an image sensor comprising a pixel array for sensing reflected electromagnetic radiation. The system includes an electromagnetic sensor for sensing energy emitted by the emitter. The system includes a controller configured to synchronize timing of the emitter and the image sensor. The system is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises electromagnetic radiation having a wavelength from about 770 nm to about 790 nm and/or from about 795 nm to about 815 nm.